Cargando…

Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1

Pseudomonas fluorescens strains are important candidates for use as biological control agents to reduce fungal diseases on crop plants. To understand the ecological success of these bacteria and for successful and stable biological control, determination of how these bacteria colonize and persist in...

Descripción completa

Detalles Bibliográficos
Autores principales: Marshall, Douglas C., Arruda, Brianna E., Silby, Mark W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471777/
https://www.ncbi.nlm.nih.gov/pubmed/32974516
http://dx.doi.org/10.1099/acmi.0.000021
_version_ 1783578839140532224
author Marshall, Douglas C.
Arruda, Brianna E.
Silby, Mark W.
author_facet Marshall, Douglas C.
Arruda, Brianna E.
Silby, Mark W.
author_sort Marshall, Douglas C.
collection PubMed
description Pseudomonas fluorescens strains are important candidates for use as biological control agents to reduce fungal diseases on crop plants. To understand the ecological success of these bacteria and for successful and stable biological control, determination of how these bacteria colonize and persist in soil environments is critical. Here we show that P. fluorescens Pf0-1 is negatively impacted by reduced water availability in soil, but adapts and persists. A pilot transcriptomic study of Pf0-1 colonizing moist and dehydrated soil was used to identify candidate genetic loci, which could play a role in the adaptation to dehydration. Genes predicted to specify alginate production were identified and chosen for functional evaluation. Using deletion mutants, predicted alginate biosynthesis genes were shown to be important for optimal colonization of moist soil, and necessary for adaptation to reduced water availability in dried soil. Our findings extend in vitro studies of water stress into a more natural system and suggest alginate may be an essential extracellular product for the lifestyle of P. fluorescens when growing in soil.
format Online
Article
Text
id pubmed-7471777
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-74717772020-09-23 Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1 Marshall, Douglas C. Arruda, Brianna E. Silby, Mark W. Access Microbiol Research Article Pseudomonas fluorescens strains are important candidates for use as biological control agents to reduce fungal diseases on crop plants. To understand the ecological success of these bacteria and for successful and stable biological control, determination of how these bacteria colonize and persist in soil environments is critical. Here we show that P. fluorescens Pf0-1 is negatively impacted by reduced water availability in soil, but adapts and persists. A pilot transcriptomic study of Pf0-1 colonizing moist and dehydrated soil was used to identify candidate genetic loci, which could play a role in the adaptation to dehydration. Genes predicted to specify alginate production were identified and chosen for functional evaluation. Using deletion mutants, predicted alginate biosynthesis genes were shown to be important for optimal colonization of moist soil, and necessary for adaptation to reduced water availability in dried soil. Our findings extend in vitro studies of water stress into a more natural system and suggest alginate may be an essential extracellular product for the lifestyle of P. fluorescens when growing in soil. Microbiology Society 2019-05-03 /pmc/articles/PMC7471777/ /pubmed/32974516 http://dx.doi.org/10.1099/acmi.0.000021 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Research Article
Marshall, Douglas C.
Arruda, Brianna E.
Silby, Mark W.
Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title_full Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title_fullStr Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title_full_unstemmed Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title_short Alginate genes are required for optimal soil colonization and persistence by Pseudomonas fluorescens Pf0-1
title_sort alginate genes are required for optimal soil colonization and persistence by pseudomonas fluorescens pf0-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471777/
https://www.ncbi.nlm.nih.gov/pubmed/32974516
http://dx.doi.org/10.1099/acmi.0.000021
work_keys_str_mv AT marshalldouglasc alginategenesarerequiredforoptimalsoilcolonizationandpersistencebypseudomonasfluorescenspf01
AT arrudabriannae alginategenesarerequiredforoptimalsoilcolonizationandpersistencebypseudomonasfluorescenspf01
AT silbymarkw alginategenesarerequiredforoptimalsoilcolonizationandpersistencebypseudomonasfluorescenspf01