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An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope

In this paper, we propose and demonstrate the use of an edge-lit volume holographic optical element (EL-VHOE) as a reference waveguide to reduce the volume of a lensless digital holographic microscope. Additionally, a hybrid lensless Fourier transform digital holography is applied to make the EL-VHO...

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Autores principales: Yu, Yeh-Wei, Sun, Ching-Cherng, Hsieh, Po-Kai, Huang, Yi-Hao, Song, Chih-Yuan, Yang, Tsung-Hsun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471914/
https://www.ncbi.nlm.nih.gov/pubmed/32884051
http://dx.doi.org/10.1038/s41598-020-71497-7
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author Yu, Yeh-Wei
Sun, Ching-Cherng
Hsieh, Po-Kai
Huang, Yi-Hao
Song, Chih-Yuan
Yang, Tsung-Hsun
author_facet Yu, Yeh-Wei
Sun, Ching-Cherng
Hsieh, Po-Kai
Huang, Yi-Hao
Song, Chih-Yuan
Yang, Tsung-Hsun
author_sort Yu, Yeh-Wei
collection PubMed
description In this paper, we propose and demonstrate the use of an edge-lit volume holographic optical element (EL-VHOE) as a reference waveguide to reduce the volume of a lensless digital holographic microscope. Additionally, a hybrid lensless Fourier transform digital holography is applied to make the EL-VHOE function as an objective turret. It used a spherical wave in the object beam of the EL-VHOE, which served as the reference beam of the microscope. Another sheared spherical wave was used to illuminate the sample. The longitudinal position of the spherical reference beam is changeable. It was shown that the tradeoff between resolution and field of view can be adjusted by changing the longitudinal position of the spherical reference beam. The corresponding experimental results matched the simulational and theoretical predictions. A resolution of approximately 3.11 μm was achieved when the object distance was 6 mm and the longitudinal distance of the spherical reference was 10 mm.
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spelling pubmed-74719142020-09-08 An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope Yu, Yeh-Wei Sun, Ching-Cherng Hsieh, Po-Kai Huang, Yi-Hao Song, Chih-Yuan Yang, Tsung-Hsun Sci Rep Article In this paper, we propose and demonstrate the use of an edge-lit volume holographic optical element (EL-VHOE) as a reference waveguide to reduce the volume of a lensless digital holographic microscope. Additionally, a hybrid lensless Fourier transform digital holography is applied to make the EL-VHOE function as an objective turret. It used a spherical wave in the object beam of the EL-VHOE, which served as the reference beam of the microscope. Another sheared spherical wave was used to illuminate the sample. The longitudinal position of the spherical reference beam is changeable. It was shown that the tradeoff between resolution and field of view can be adjusted by changing the longitudinal position of the spherical reference beam. The corresponding experimental results matched the simulational and theoretical predictions. A resolution of approximately 3.11 μm was achieved when the object distance was 6 mm and the longitudinal distance of the spherical reference was 10 mm. Nature Publishing Group UK 2020-09-03 /pmc/articles/PMC7471914/ /pubmed/32884051 http://dx.doi.org/10.1038/s41598-020-71497-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yu, Yeh-Wei
Sun, Ching-Cherng
Hsieh, Po-Kai
Huang, Yi-Hao
Song, Chih-Yuan
Yang, Tsung-Hsun
An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title_full An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title_fullStr An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title_full_unstemmed An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title_short An edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
title_sort edge-lit volume holographic optical element for an objective turret in a lensless digital holographic microscope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471914/
https://www.ncbi.nlm.nih.gov/pubmed/32884051
http://dx.doi.org/10.1038/s41598-020-71497-7
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