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A CRISPR activation and interference toolkit for industrial Saccharomyces cerevisiae strain KE6-12

Recent advances in CRISPR/Cas9 based genome editing have considerably advanced genetic engineering of industrial yeast strains. In this study, we report the construction and characterization of a toolkit for CRISPR activation and interference (CRISPRa/i) for a polyploid industrial yeast strain. In t...

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Detalles Bibliográficos
Autores principales: Cámara, Elena, Lenitz, Ibai, Nygård, Yvonne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471924/
https://www.ncbi.nlm.nih.gov/pubmed/32884066
http://dx.doi.org/10.1038/s41598-020-71648-w