Micromorphological details and identification of chitinous wall structures in Rapana venosa (Gastropoda, Mollusca) egg capsules

The present study evaluated the structural and ultrastructural characteristics of Rapana venosa egg capsules, starting from observations of their antifouling activity and mechanical resistance to water currents in mid-shore habitats. Optical microscopy, epifluorescence, and electron microscopy were used to evaluate the surface and structure of the R. venosa egg capsules. These measurements revealed an internal multilamellar structure of the capsule wall with in-plane distributions of layers with various orientations. It was found that the walls contained vacuolar structures in the median layer, which provided the particular characteristics. Mechanical, viscoelastic and swelling measurements were also carried out. This study revealed the presence and distribution of chitosan in the capsule of R. venosa. Chitosan identification in the egg capsule wall structure was carried out through SEM–EDX measurements, colorimetric assays, FT-IR spectra and physical–chemical tests. The biopolymer presence in the capsule walls may explain the properties of their surfaces as well as the mechanical resistance of the capsule and its resistance to chemical variations in the living environment.