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Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study

Both mucosal and cutaneous Human Papillomaviruses (HPVs) can be detected in the oral cavity, but investigations regarding the epidemiology of cutaneous HPVs at this site are scarce. We assessed mucosal (alpha) and cutaneous (beta and gamma) HPV infection in oral samples of HIV-infected and uninfecte...

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Autores principales: Gheit, Tarik, Rollo, Francesca, Brancaccio, Rosario N, Robitaille, Alexis, Galati, Luisa, Giuliani, Massimo, Latini, Alessandra, Pichi, Barbara, Benevolo, Maria, Cuenin, Cyrille, McKay-Chopin, Sandrine, Pellini, Raul, Cristaudo, Antonio, Morrone, Aldo, Tommasino, Massimo, Donà, Maria Gabriella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472018/
https://www.ncbi.nlm.nih.gov/pubmed/32824507
http://dx.doi.org/10.3390/v12080899
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author Gheit, Tarik
Rollo, Francesca
Brancaccio, Rosario N
Robitaille, Alexis
Galati, Luisa
Giuliani, Massimo
Latini, Alessandra
Pichi, Barbara
Benevolo, Maria
Cuenin, Cyrille
McKay-Chopin, Sandrine
Pellini, Raul
Cristaudo, Antonio
Morrone, Aldo
Tommasino, Massimo
Donà, Maria Gabriella
author_facet Gheit, Tarik
Rollo, Francesca
Brancaccio, Rosario N
Robitaille, Alexis
Galati, Luisa
Giuliani, Massimo
Latini, Alessandra
Pichi, Barbara
Benevolo, Maria
Cuenin, Cyrille
McKay-Chopin, Sandrine
Pellini, Raul
Cristaudo, Antonio
Morrone, Aldo
Tommasino, Massimo
Donà, Maria Gabriella
author_sort Gheit, Tarik
collection PubMed
description Both mucosal and cutaneous Human Papillomaviruses (HPVs) can be detected in the oral cavity, but investigations regarding the epidemiology of cutaneous HPVs at this site are scarce. We assessed mucosal (alpha) and cutaneous (beta and gamma) HPV infection in oral samples of HIV-infected and uninfected men who have sex with men (MSM). Oral rinse-and-gargles were collected from 310 MSM. Alpha HPVs were detected using the Linear Array, whereas beta and gamma HPVs were detected using multiplex PCR and Luminex technology. An amplicon-based next-generation sequencing (NGS) protocol was applied to a subset of samples collected from 30 HIV-uninfected and 30 HIV-infected MSM. Beta HPVs were significantly more common than alpha types (53.8% vs. 23.9% for HIV-infected subjects, p < 0.0001; 50.3% vs. 17.1% for HIV-uninfected subjects, p < 0.0001). Gamma HPVs were also frequently detected (30.8% and 25.9% in HIV-infected and uninfected MSM, respectively). NGS produced 2,620,725 reads representative of 146 known HPVs (16 alpha-PVs, 53 beta-PVs, 76 gamma-PVs, one unclassified) and eight putative new HPVs, taxonomically assigned to the beta genus. The oral cavity contains a wide spectrum of HPVs, with beta types representing the predominant genus. The prevalence of beta and gamma HPVs is high even in immunorestored HIV-infected individuals. NGS confirmed the abundance of cutaneous HPVs and identified some putative novel beta HPVs. This study confirms that cutaneous HPVs are frequently present at mucosal sites and highlights that their pathological role deserves further investigation since it may not be limited to skin lesions.
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spelling pubmed-74720182020-09-17 Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study Gheit, Tarik Rollo, Francesca Brancaccio, Rosario N Robitaille, Alexis Galati, Luisa Giuliani, Massimo Latini, Alessandra Pichi, Barbara Benevolo, Maria Cuenin, Cyrille McKay-Chopin, Sandrine Pellini, Raul Cristaudo, Antonio Morrone, Aldo Tommasino, Massimo Donà, Maria Gabriella Viruses Article Both mucosal and cutaneous Human Papillomaviruses (HPVs) can be detected in the oral cavity, but investigations regarding the epidemiology of cutaneous HPVs at this site are scarce. We assessed mucosal (alpha) and cutaneous (beta and gamma) HPV infection in oral samples of HIV-infected and uninfected men who have sex with men (MSM). Oral rinse-and-gargles were collected from 310 MSM. Alpha HPVs were detected using the Linear Array, whereas beta and gamma HPVs were detected using multiplex PCR and Luminex technology. An amplicon-based next-generation sequencing (NGS) protocol was applied to a subset of samples collected from 30 HIV-uninfected and 30 HIV-infected MSM. Beta HPVs were significantly more common than alpha types (53.8% vs. 23.9% for HIV-infected subjects, p < 0.0001; 50.3% vs. 17.1% for HIV-uninfected subjects, p < 0.0001). Gamma HPVs were also frequently detected (30.8% and 25.9% in HIV-infected and uninfected MSM, respectively). NGS produced 2,620,725 reads representative of 146 known HPVs (16 alpha-PVs, 53 beta-PVs, 76 gamma-PVs, one unclassified) and eight putative new HPVs, taxonomically assigned to the beta genus. The oral cavity contains a wide spectrum of HPVs, with beta types representing the predominant genus. The prevalence of beta and gamma HPVs is high even in immunorestored HIV-infected individuals. NGS confirmed the abundance of cutaneous HPVs and identified some putative novel beta HPVs. This study confirms that cutaneous HPVs are frequently present at mucosal sites and highlights that their pathological role deserves further investigation since it may not be limited to skin lesions. MDPI 2020-08-17 /pmc/articles/PMC7472018/ /pubmed/32824507 http://dx.doi.org/10.3390/v12080899 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gheit, Tarik
Rollo, Francesca
Brancaccio, Rosario N
Robitaille, Alexis
Galati, Luisa
Giuliani, Massimo
Latini, Alessandra
Pichi, Barbara
Benevolo, Maria
Cuenin, Cyrille
McKay-Chopin, Sandrine
Pellini, Raul
Cristaudo, Antonio
Morrone, Aldo
Tommasino, Massimo
Donà, Maria Gabriella
Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title_full Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title_fullStr Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title_full_unstemmed Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title_short Oral Infection by Mucosal and Cutaneous Human Papillomaviruses in the Men Who Have Sex with Men from the OHMAR Study
title_sort oral infection by mucosal and cutaneous human papillomaviruses in the men who have sex with men from the ohmar study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472018/
https://www.ncbi.nlm.nih.gov/pubmed/32824507
http://dx.doi.org/10.3390/v12080899
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