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Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B

Human herpes simplex viruses (HSV) 1 and 2 are extremely common human pathogens with overlapping disease spectra. Infections due to HSV-1 and HSV-2 are distinguished in clinical settings using sequence-based “typing” assays. Here we describe a case of HSV mistyping caused by a previously undescribed...

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Autores principales: Casto, Amanda M., Huang, Meei-Li W., Xie, Hong, Jerome, Keith R., Wald, Anna, Johnston, Christine M., Greninger, Alexander L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472045/
https://www.ncbi.nlm.nih.gov/pubmed/32781734
http://dx.doi.org/10.3390/v12080860
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author Casto, Amanda M.
Huang, Meei-Li W.
Xie, Hong
Jerome, Keith R.
Wald, Anna
Johnston, Christine M.
Greninger, Alexander L.
author_facet Casto, Amanda M.
Huang, Meei-Li W.
Xie, Hong
Jerome, Keith R.
Wald, Anna
Johnston, Christine M.
Greninger, Alexander L.
author_sort Casto, Amanda M.
collection PubMed
description Human herpes simplex viruses (HSV) 1 and 2 are extremely common human pathogens with overlapping disease spectra. Infections due to HSV-1 and HSV-2 are distinguished in clinical settings using sequence-based “typing” assays. Here we describe a case of HSV mistyping caused by a previously undescribed HSV-1 × HSV-2 recombination event in UL27, the HSV gene that encodes glycoprotein B. This is the first documented case of HSV mistyping caused by an HSV-1 × HSV-2 recombination event and the first description of an HSV interspecies recombination event in UL27, which is frequently used as a target for diagnostics and experimental therapeutics. We also review the primer and probe target sequences for a commonly used HSV typing assay from nearly 700 HSV-1 and HSV-2 samples and find that about 4% of HSV-1 samples have a single nucleotide change in at least one of these loci, which could impact assay performance. Our findings illustrate how knowledge of naturally occurring genomic variation in HSV-1 and HSV-2 is essential for the design and interpretation of molecular diagnostics for these viruses.
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spelling pubmed-74720452020-09-17 Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B Casto, Amanda M. Huang, Meei-Li W. Xie, Hong Jerome, Keith R. Wald, Anna Johnston, Christine M. Greninger, Alexander L. Viruses Communication Human herpes simplex viruses (HSV) 1 and 2 are extremely common human pathogens with overlapping disease spectra. Infections due to HSV-1 and HSV-2 are distinguished in clinical settings using sequence-based “typing” assays. Here we describe a case of HSV mistyping caused by a previously undescribed HSV-1 × HSV-2 recombination event in UL27, the HSV gene that encodes glycoprotein B. This is the first documented case of HSV mistyping caused by an HSV-1 × HSV-2 recombination event and the first description of an HSV interspecies recombination event in UL27, which is frequently used as a target for diagnostics and experimental therapeutics. We also review the primer and probe target sequences for a commonly used HSV typing assay from nearly 700 HSV-1 and HSV-2 samples and find that about 4% of HSV-1 samples have a single nucleotide change in at least one of these loci, which could impact assay performance. Our findings illustrate how knowledge of naturally occurring genomic variation in HSV-1 and HSV-2 is essential for the design and interpretation of molecular diagnostics for these viruses. MDPI 2020-08-06 /pmc/articles/PMC7472045/ /pubmed/32781734 http://dx.doi.org/10.3390/v12080860 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Casto, Amanda M.
Huang, Meei-Li W.
Xie, Hong
Jerome, Keith R.
Wald, Anna
Johnston, Christine M.
Greninger, Alexander L.
Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title_full Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title_fullStr Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title_full_unstemmed Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title_short Herpes Simplex Virus Mistyping due to HSV-1 × HSV-2 Interspecies Recombination in Viral Gene Encoding Glycoprotein B
title_sort herpes simplex virus mistyping due to hsv-1 × hsv-2 interspecies recombination in viral gene encoding glycoprotein b
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472045/
https://www.ncbi.nlm.nih.gov/pubmed/32781734
http://dx.doi.org/10.3390/v12080860
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