Cargando…
Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil
The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika,...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472125/ https://www.ncbi.nlm.nih.gov/pubmed/32823806 http://dx.doi.org/10.3390/v12080889 |
_version_ | 1783578917094817792 |
---|---|
author | Queiroz, Jackson Alves da Silva Botelho-Souza, Luan Felipo Nogueira-Lima, Felipe Souza Rampazzo, Rita de Cássia Pontello Krieger, Marco Aurélio Zambenedetti, Miriam Ribas Marchini, Fabricio Klerinton Borghetti, Ivo Alberto Pereira, Dhelio Batista Salcedo, Juan Miguel Vilalobos Vieira, Deusilene Souza dos Santos, Alcione de Oliveira |
author_facet | Queiroz, Jackson Alves da Silva Botelho-Souza, Luan Felipo Nogueira-Lima, Felipe Souza Rampazzo, Rita de Cássia Pontello Krieger, Marco Aurélio Zambenedetti, Miriam Ribas Marchini, Fabricio Klerinton Borghetti, Ivo Alberto Pereira, Dhelio Batista Salcedo, Juan Miguel Vilalobos Vieira, Deusilene Souza dos Santos, Alcione de Oliveira |
author_sort | Queiroz, Jackson Alves da Silva |
collection | PubMed |
description | The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika, Dengue (serotypes 1–4), Chikungunya, Mayaro and Oropouche viruses. Positive samples were amplified by conventional PCR and sequenced utilizing the Sanger method. The obtained sequences were aligned, and an evolutionary analysis was carried out using Bayesian inference. A total of 308 samples were tested. Of this total, 20 had a detectable viral load for Dengue, being detected DENV1 (18/20), co-infection DENV1 and DENV2 (1/20) and DENV4 (1/20). For Dengue serotype 3 and for the CHIKV, ZIKV, MAYV and OROV viruses, no individuals with a detectable viral load were found. A total of 9 of these samples were magnified by conventional PCR for sequencing. Of these, 6 were successfully sequenced and, according to the evolutionary profile, 5 corresponded to serotype DENV-1 genotype V, and 1 to serotype DENV-4 genotype II. In the study, we demonstrate co-circulation of the DENV-1 genotype V and the DENV-4 genotype II. Co-circulation of several DENV serotypes in the same city poses a risk to the population and is correlated with the increase of the most severe forms of the disease. Similarly, co-circulation of genetically distinct DENV and the occurrence of simultaneous infections can affect recombination events and lead to the emergence of more virulent isolates. |
format | Online Article Text |
id | pubmed-7472125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74721252020-09-04 Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil Queiroz, Jackson Alves da Silva Botelho-Souza, Luan Felipo Nogueira-Lima, Felipe Souza Rampazzo, Rita de Cássia Pontello Krieger, Marco Aurélio Zambenedetti, Miriam Ribas Marchini, Fabricio Klerinton Borghetti, Ivo Alberto Pereira, Dhelio Batista Salcedo, Juan Miguel Vilalobos Vieira, Deusilene Souza dos Santos, Alcione de Oliveira Viruses Article The purpose of the study was to classify, through phylogenetic analyses, the main arboviruses that have been isolated in the metropolitan region of Porto Velho, Rondônia, Brazil. Serum samples from patients with symptoms suggesting arboviruses were collected and tested by One Step RT-qPCR for Zika, Dengue (serotypes 1–4), Chikungunya, Mayaro and Oropouche viruses. Positive samples were amplified by conventional PCR and sequenced utilizing the Sanger method. The obtained sequences were aligned, and an evolutionary analysis was carried out using Bayesian inference. A total of 308 samples were tested. Of this total, 20 had a detectable viral load for Dengue, being detected DENV1 (18/20), co-infection DENV1 and DENV2 (1/20) and DENV4 (1/20). For Dengue serotype 3 and for the CHIKV, ZIKV, MAYV and OROV viruses, no individuals with a detectable viral load were found. A total of 9 of these samples were magnified by conventional PCR for sequencing. Of these, 6 were successfully sequenced and, according to the evolutionary profile, 5 corresponded to serotype DENV-1 genotype V, and 1 to serotype DENV-4 genotype II. In the study, we demonstrate co-circulation of the DENV-1 genotype V and the DENV-4 genotype II. Co-circulation of several DENV serotypes in the same city poses a risk to the population and is correlated with the increase of the most severe forms of the disease. Similarly, co-circulation of genetically distinct DENV and the occurrence of simultaneous infections can affect recombination events and lead to the emergence of more virulent isolates. MDPI 2020-08-14 /pmc/articles/PMC7472125/ /pubmed/32823806 http://dx.doi.org/10.3390/v12080889 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Queiroz, Jackson Alves da Silva Botelho-Souza, Luan Felipo Nogueira-Lima, Felipe Souza Rampazzo, Rita de Cássia Pontello Krieger, Marco Aurélio Zambenedetti, Miriam Ribas Marchini, Fabricio Klerinton Borghetti, Ivo Alberto Pereira, Dhelio Batista Salcedo, Juan Miguel Vilalobos Vieira, Deusilene Souza dos Santos, Alcione de Oliveira Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title_full | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title_fullStr | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title_full_unstemmed | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title_short | Phylogenetic Characterization of Arboviruses in Patients Suffering from Acute Fever in Rondônia, Brazil |
title_sort | phylogenetic characterization of arboviruses in patients suffering from acute fever in rondônia, brazil |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472125/ https://www.ncbi.nlm.nih.gov/pubmed/32823806 http://dx.doi.org/10.3390/v12080889 |
work_keys_str_mv | AT queirozjacksonalvesdasilva phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT botelhosouzaluanfelipo phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT nogueiralimafelipesouza phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT rampazzoritadecassiapontello phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT kriegermarcoaurelio phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT zambenedettimiriamribas phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT marchinifabricioklerinton phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT borghettiivoalberto phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT pereiradheliobatista phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT salcedojuanmiguelvilalobos phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT vieiradeusilenesouza phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil AT dossantosalcionedeoliveira phylogeneticcharacterizationofarbovirusesinpatientssufferingfromacutefeverinrondoniabrazil |