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Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens

This paper reports the first results on obtaining an enzyme preparation that might be promising for the simultaneous decontamination of plant feeds contaminated with a polyketide fusariotoxin, zearalenone (ZEN), and enhancing the availability of their nutritional components. A novel ZEN-specific lac...

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Autores principales: Shcherbakova, Larisa, Rozhkova, Alexandra, Osipov, Dmitrii, Zorov, Ivan, Mikityuk, Oleg, Statsyuk, Natalia, Sinitsyna, Olga, Dzhavakhiya, Vitaly, Sinitsyn, Arkady
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472149/
https://www.ncbi.nlm.nih.gov/pubmed/32722498
http://dx.doi.org/10.3390/toxins12080475
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author Shcherbakova, Larisa
Rozhkova, Alexandra
Osipov, Dmitrii
Zorov, Ivan
Mikityuk, Oleg
Statsyuk, Natalia
Sinitsyna, Olga
Dzhavakhiya, Vitaly
Sinitsyn, Arkady
author_facet Shcherbakova, Larisa
Rozhkova, Alexandra
Osipov, Dmitrii
Zorov, Ivan
Mikityuk, Oleg
Statsyuk, Natalia
Sinitsyna, Olga
Dzhavakhiya, Vitaly
Sinitsyn, Arkady
author_sort Shcherbakova, Larisa
collection PubMed
description This paper reports the first results on obtaining an enzyme preparation that might be promising for the simultaneous decontamination of plant feeds contaminated with a polyketide fusariotoxin, zearalenone (ZEN), and enhancing the availability of their nutritional components. A novel ZEN-specific lactonohydrolase (ZHD) was expressed in a Penicillium canescens strain PCA-10 that was developed previously as a producer of different hydrolytic enzymes for feed biorefinery. The recombinant ZHD secreted by transformed fungal clones into culture liquid was shown to remove the toxin from model solutions, and was able to decontaminate wheat grain artificially infected with a zearalenone-producing Fusarium culmorum. The dynamics of ZEN degradation depending on the temperature and pH of the incubation media was investigated, and the optimal values of these parameters (pH 8.5, 30 °C) for the ZHD-containing enzyme preparation (PR-ZHD) were determined. Under these conditions, the 3 h co-incubation of ZEN and PR-ZHD resulted in a complete removal of the toxin from the model solutions, while the PR-ZHD addition (8 mg/g of dried grain) to flour samples prepared from the infected ZEN-polluted grain (about 16 µg/g) completely decontaminated the samples after an overnight exposure.
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spelling pubmed-74721492020-09-04 Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens Shcherbakova, Larisa Rozhkova, Alexandra Osipov, Dmitrii Zorov, Ivan Mikityuk, Oleg Statsyuk, Natalia Sinitsyna, Olga Dzhavakhiya, Vitaly Sinitsyn, Arkady Toxins (Basel) Article This paper reports the first results on obtaining an enzyme preparation that might be promising for the simultaneous decontamination of plant feeds contaminated with a polyketide fusariotoxin, zearalenone (ZEN), and enhancing the availability of their nutritional components. A novel ZEN-specific lactonohydrolase (ZHD) was expressed in a Penicillium canescens strain PCA-10 that was developed previously as a producer of different hydrolytic enzymes for feed biorefinery. The recombinant ZHD secreted by transformed fungal clones into culture liquid was shown to remove the toxin from model solutions, and was able to decontaminate wheat grain artificially infected with a zearalenone-producing Fusarium culmorum. The dynamics of ZEN degradation depending on the temperature and pH of the incubation media was investigated, and the optimal values of these parameters (pH 8.5, 30 °C) for the ZHD-containing enzyme preparation (PR-ZHD) were determined. Under these conditions, the 3 h co-incubation of ZEN and PR-ZHD resulted in a complete removal of the toxin from the model solutions, while the PR-ZHD addition (8 mg/g of dried grain) to flour samples prepared from the infected ZEN-polluted grain (about 16 µg/g) completely decontaminated the samples after an overnight exposure. MDPI 2020-07-25 /pmc/articles/PMC7472149/ /pubmed/32722498 http://dx.doi.org/10.3390/toxins12080475 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shcherbakova, Larisa
Rozhkova, Alexandra
Osipov, Dmitrii
Zorov, Ivan
Mikityuk, Oleg
Statsyuk, Natalia
Sinitsyna, Olga
Dzhavakhiya, Vitaly
Sinitsyn, Arkady
Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title_full Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title_fullStr Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title_full_unstemmed Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title_short Effective Zearalenone Degradation in Model Solutions and Infected Wheat Grain Using a Novel Heterologous Lactonohydrolase Secreted by Recombinant Penicillium canescens
title_sort effective zearalenone degradation in model solutions and infected wheat grain using a novel heterologous lactonohydrolase secreted by recombinant penicillium canescens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472149/
https://www.ncbi.nlm.nih.gov/pubmed/32722498
http://dx.doi.org/10.3390/toxins12080475
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