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Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D

RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production. The 3′ untranslated region (3′UTR) of Tobacco necrosis virus strain D (TNV-D; genus Betanecrovirus, family Tombu...

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Autores principales: Newburn, Laura R., Wu, Baodong, White, K. Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472153/
https://www.ncbi.nlm.nih.gov/pubmed/32781505
http://dx.doi.org/10.3390/v12080856
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author Newburn, Laura R.
Wu, Baodong
White, K. Andrew
author_facet Newburn, Laura R.
Wu, Baodong
White, K. Andrew
author_sort Newburn, Laura R.
collection PubMed
description RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production. The 3′ untranslated region (3′UTR) of Tobacco necrosis virus strain D (TNV-D; genus Betanecrovirus, family Tombusviridae) contains several well studied regulatory RNA elements. Here, we explore a previously unexamined region of the viral 3′UTR, the sequence located upstream of the 3′-cap independent translation enhancer (3′CITE). Our results indicate that (i) a long-range RNA–RNA interaction between an internal RNA element and the 3′UTR facilitates translational readthrough, and may also promote viral RNA synthesis; (ii) a conserved RNA hairpin, SLX, is required for efficient genome accumulation; and (iii) an adenine-rich region upstream of the 3′CITE is dispensable, but can modulate genome accumulation. These findings identified novel regulatory RNA elements in the 3′UTR of the TNV-D genome that are important for virus survival.
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spelling pubmed-74721532020-09-04 Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D Newburn, Laura R. Wu, Baodong White, K. Andrew Viruses Article RNA elements in the untranslated regions of plus-strand RNA viruses can control a variety of viral processes including translation, replication, packaging, and subgenomic mRNA production. The 3′ untranslated region (3′UTR) of Tobacco necrosis virus strain D (TNV-D; genus Betanecrovirus, family Tombusviridae) contains several well studied regulatory RNA elements. Here, we explore a previously unexamined region of the viral 3′UTR, the sequence located upstream of the 3′-cap independent translation enhancer (3′CITE). Our results indicate that (i) a long-range RNA–RNA interaction between an internal RNA element and the 3′UTR facilitates translational readthrough, and may also promote viral RNA synthesis; (ii) a conserved RNA hairpin, SLX, is required for efficient genome accumulation; and (iii) an adenine-rich region upstream of the 3′CITE is dispensable, but can modulate genome accumulation. These findings identified novel regulatory RNA elements in the 3′UTR of the TNV-D genome that are important for virus survival. MDPI 2020-08-06 /pmc/articles/PMC7472153/ /pubmed/32781505 http://dx.doi.org/10.3390/v12080856 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Newburn, Laura R.
Wu, Baodong
White, K. Andrew
Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title_full Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title_fullStr Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title_full_unstemmed Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title_short Investigation of Novel RNA Elements in the 3′UTR of Tobacco Necrosis Virus-D
title_sort investigation of novel rna elements in the 3′utr of tobacco necrosis virus-d
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472153/
https://www.ncbi.nlm.nih.gov/pubmed/32781505
http://dx.doi.org/10.3390/v12080856
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