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Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor

In this article we present the development of a biosensor system that integrates nanotechnology, optomechanics and a spectral detection algorithm for sensitive quantification of antibiotic residues in raw milk of cow. Firstly, nanobiosensors were designed and synthesized by chemically bonding gold n...

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Autores principales: Gutiérrez, Pablo, Godoy, Sebastián E., Torres, Sergio, Oyarzún, Patricio, Sanhueza, Ignacio, Díaz-García, Victor, Contreras-Trigo, Braulio, Coelho, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472326/
https://www.ncbi.nlm.nih.gov/pubmed/32823811
http://dx.doi.org/10.3390/s20164552
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author Gutiérrez, Pablo
Godoy, Sebastián E.
Torres, Sergio
Oyarzún, Patricio
Sanhueza, Ignacio
Díaz-García, Victor
Contreras-Trigo, Braulio
Coelho, Pablo
author_facet Gutiérrez, Pablo
Godoy, Sebastián E.
Torres, Sergio
Oyarzún, Patricio
Sanhueza, Ignacio
Díaz-García, Victor
Contreras-Trigo, Braulio
Coelho, Pablo
author_sort Gutiérrez, Pablo
collection PubMed
description In this article we present the development of a biosensor system that integrates nanotechnology, optomechanics and a spectral detection algorithm for sensitive quantification of antibiotic residues in raw milk of cow. Firstly, nanobiosensors were designed and synthesized by chemically bonding gold nanoparticles (AuNPs) with aptamer bioreceptors highly selective for four widely used antibiotics in the field of veterinary medicine, namely, Kanamycin, Ampicillin, Oxytetracycline and Sulfadimethoxine. When molecules of the antibiotics are present in the milk sample, the interaction with the aptamers induces random AuNP aggregation. This phenomenon modifies the initial absorption spectrum of the milk sample without antibiotics, producing spectral features that indicate both the presence of antibiotics and, to some extent, its concentration. Secondly, we designed and constructed an electro-opto-mechanic device that performs automatic high-resolution spectral data acquisition in a wavelength range of 400 to 800 nm. Thirdly, the acquired spectra were processed by a machine-learning algorithm that is embedded into the acquisition hardware to determine the presence and concentration ranges of the antibiotics. Our approach outperformed state-of-the-art standardized techniques (based on the 520/620 nm ratio) for antibiotic detection, both in speed and in sensitivity.
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spelling pubmed-74723262020-09-04 Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor Gutiérrez, Pablo Godoy, Sebastián E. Torres, Sergio Oyarzún, Patricio Sanhueza, Ignacio Díaz-García, Victor Contreras-Trigo, Braulio Coelho, Pablo Sensors (Basel) Letter In this article we present the development of a biosensor system that integrates nanotechnology, optomechanics and a spectral detection algorithm for sensitive quantification of antibiotic residues in raw milk of cow. Firstly, nanobiosensors were designed and synthesized by chemically bonding gold nanoparticles (AuNPs) with aptamer bioreceptors highly selective for four widely used antibiotics in the field of veterinary medicine, namely, Kanamycin, Ampicillin, Oxytetracycline and Sulfadimethoxine. When molecules of the antibiotics are present in the milk sample, the interaction with the aptamers induces random AuNP aggregation. This phenomenon modifies the initial absorption spectrum of the milk sample without antibiotics, producing spectral features that indicate both the presence of antibiotics and, to some extent, its concentration. Secondly, we designed and constructed an electro-opto-mechanic device that performs automatic high-resolution spectral data acquisition in a wavelength range of 400 to 800 nm. Thirdly, the acquired spectra were processed by a machine-learning algorithm that is embedded into the acquisition hardware to determine the presence and concentration ranges of the antibiotics. Our approach outperformed state-of-the-art standardized techniques (based on the 520/620 nm ratio) for antibiotic detection, both in speed and in sensitivity. MDPI 2020-08-14 /pmc/articles/PMC7472326/ /pubmed/32823811 http://dx.doi.org/10.3390/s20164552 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Letter
Gutiérrez, Pablo
Godoy, Sebastián E.
Torres, Sergio
Oyarzún, Patricio
Sanhueza, Ignacio
Díaz-García, Victor
Contreras-Trigo, Braulio
Coelho, Pablo
Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title_full Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title_fullStr Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title_full_unstemmed Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title_short Improved Antibiotic Detection in Raw Milk Using Machine Learning Tools over the Absorption Spectra of a Problem-Specific Nanobiosensor
title_sort improved antibiotic detection in raw milk using machine learning tools over the absorption spectra of a problem-specific nanobiosensor
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472326/
https://www.ncbi.nlm.nih.gov/pubmed/32823811
http://dx.doi.org/10.3390/s20164552
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