Cargando…

Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells

Reactivation of BK polyomavirus (BKPyV) infection is frequently increasing in transplant recipients treated with potent immunosuppressants and highlights the importance of immune system components in controlling viral reactivation. However, the immune response to BKPyV in general and the role of ant...

Descripción completa

Detalles Bibliográficos
Autores principales: Fiore, Tony, Martin, Elodie, Descamps, Véronique, Brochot, Etienne, Morel, Virginie, Handala, Lynda, Dakroub, Fatima, Castelain, Sandrine, Duverlie, Gilles, Helle, François, François, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472348/
https://www.ncbi.nlm.nih.gov/pubmed/32784805
http://dx.doi.org/10.3390/v12080865
_version_ 1783578968101748736
author Fiore, Tony
Martin, Elodie
Descamps, Véronique
Brochot, Etienne
Morel, Virginie
Handala, Lynda
Dakroub, Fatima
Castelain, Sandrine
Duverlie, Gilles
Helle, François
François, Catherine
author_facet Fiore, Tony
Martin, Elodie
Descamps, Véronique
Brochot, Etienne
Morel, Virginie
Handala, Lynda
Dakroub, Fatima
Castelain, Sandrine
Duverlie, Gilles
Helle, François
François, Catherine
author_sort Fiore, Tony
collection PubMed
description Reactivation of BK polyomavirus (BKPyV) infection is frequently increasing in transplant recipients treated with potent immunosuppressants and highlights the importance of immune system components in controlling viral reactivation. However, the immune response to BKPyV in general and the role of antiviral cytokines in infection control in particular are poorly understood. Here, we investigated the efficacy of interferons (IFN) alpha, lambda and gamma with regard to the BKPyV multiplication in Vero cells. Treatment with IFN-gamma inhibited the expression of the viral protein VP1 in a dose-dependent manner and decreased the expression of early and late viral transcripts. Viral inhibition by IFN-gamma was confirmed in human cells (Caki-1 cells and renal proximal tubular epithelial cells). One of the IFN-stimulated genes most strongly induced by IFN-gamma was the coding for the enzyme indoleamine 2,3 dioxygenase (IDO), which is known to limit viral replication and regulates the host immune system. The antiviral activity induced by IFN-gamma could be reversed by the addition of an IDO inhibitor, indicating that IDO has a specific role in anti-BKPyV activity. A better understanding of the action mechanism of these IFN-gamma-induced antiviral proteins might facilitate the development of novel therapeutic strategies.
format Online
Article
Text
id pubmed-7472348
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74723482020-09-04 Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells Fiore, Tony Martin, Elodie Descamps, Véronique Brochot, Etienne Morel, Virginie Handala, Lynda Dakroub, Fatima Castelain, Sandrine Duverlie, Gilles Helle, François François, Catherine Viruses Article Reactivation of BK polyomavirus (BKPyV) infection is frequently increasing in transplant recipients treated with potent immunosuppressants and highlights the importance of immune system components in controlling viral reactivation. However, the immune response to BKPyV in general and the role of antiviral cytokines in infection control in particular are poorly understood. Here, we investigated the efficacy of interferons (IFN) alpha, lambda and gamma with regard to the BKPyV multiplication in Vero cells. Treatment with IFN-gamma inhibited the expression of the viral protein VP1 in a dose-dependent manner and decreased the expression of early and late viral transcripts. Viral inhibition by IFN-gamma was confirmed in human cells (Caki-1 cells and renal proximal tubular epithelial cells). One of the IFN-stimulated genes most strongly induced by IFN-gamma was the coding for the enzyme indoleamine 2,3 dioxygenase (IDO), which is known to limit viral replication and regulates the host immune system. The antiviral activity induced by IFN-gamma could be reversed by the addition of an IDO inhibitor, indicating that IDO has a specific role in anti-BKPyV activity. A better understanding of the action mechanism of these IFN-gamma-induced antiviral proteins might facilitate the development of novel therapeutic strategies. MDPI 2020-08-07 /pmc/articles/PMC7472348/ /pubmed/32784805 http://dx.doi.org/10.3390/v12080865 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fiore, Tony
Martin, Elodie
Descamps, Véronique
Brochot, Etienne
Morel, Virginie
Handala, Lynda
Dakroub, Fatima
Castelain, Sandrine
Duverlie, Gilles
Helle, François
François, Catherine
Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title_full Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title_fullStr Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title_full_unstemmed Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title_short Indoleamine 2,3-Dioxygenase Is Involved in Interferon Gamma’s Anti-BKPyV Activity in Renal Cells
title_sort indoleamine 2,3-dioxygenase is involved in interferon gamma’s anti-bkpyv activity in renal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472348/
https://www.ncbi.nlm.nih.gov/pubmed/32784805
http://dx.doi.org/10.3390/v12080865
work_keys_str_mv AT fioretony indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT martinelodie indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT descampsveronique indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT brochotetienne indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT morelvirginie indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT handalalynda indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT dakroubfatima indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT castelainsandrine indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT duverliegilles indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT hellefrancois indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells
AT francoiscatherine indoleamine23dioxygenaseisinvolvedininterferongammasantibkpyvactivityinrenalcells