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Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species

In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Moz...

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Autores principales: Kuna, Ewelina, Bocian, Aleksandra, Hus, Konrad K., Petrilla, Vladimir, Petrillova, Monika, Legath, Jaroslav, Lewinska, Anna, Wnuk, Maciej
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472363/
https://www.ncbi.nlm.nih.gov/pubmed/32759763
http://dx.doi.org/10.3390/toxins12080500
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author Kuna, Ewelina
Bocian, Aleksandra
Hus, Konrad K.
Petrilla, Vladimir
Petrillova, Monika
Legath, Jaroslav
Lewinska, Anna
Wnuk, Maciej
author_facet Kuna, Ewelina
Bocian, Aleksandra
Hus, Konrad K.
Petrilla, Vladimir
Petrillova, Monika
Legath, Jaroslav
Lewinska, Anna
Wnuk, Maciej
author_sort Kuna, Ewelina
collection PubMed
description In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three Candida species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of C. albicans and C. tropicalis, affected cell cycle of C. albicans, inhibited C. tropicalis-based biofilm formation and promoted oxidative stress in C. albicans, C. glabrata and C. tropicalis cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three Candida species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of Candida species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate Candida pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments.
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spelling pubmed-74723632020-09-04 Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species Kuna, Ewelina Bocian, Aleksandra Hus, Konrad K. Petrilla, Vladimir Petrillova, Monika Legath, Jaroslav Lewinska, Anna Wnuk, Maciej Toxins (Basel) Communication In contrast to comprehensively investigated antibacterial activity of snake venoms, namely crude venoms and their selected components, little is known about antifungal properties of elapid snake venoms. In the present study, the proteome of two venoms of red spitting cobra Naja pallida (NPV) and Mozambique spitting cobra Naja mossambica (NMV) was characterized using LC-MS/MS approach, and the antifungal activity of crude venoms against three Candida species was established. A complex response to venom treatment was revealed. NPV and NMV, when used at relatively high concentrations, decreased cell viability of C. albicans and C. tropicalis, affected cell cycle of C. albicans, inhibited C. tropicalis-based biofilm formation and promoted oxidative stress in C. albicans, C. glabrata and C. tropicalis cells. NPV and NMV also modulated ammonia pulses during colony development and aging in three Candida species. All these observations provide evidence that NPV and NMV may diminish selected pathogenic features of Candida species. However, NPV and NMV also promoted the secretion of extracellular phospholipases that may facilitate Candida pathogenicity and limit their usefulness as anti-candidal agents. In conclusion, antifungal activity of snake venoms should be studied with great caution and a plethora of pathogenic biomarkers should be considered in the future experiments. MDPI 2020-08-04 /pmc/articles/PMC7472363/ /pubmed/32759763 http://dx.doi.org/10.3390/toxins12080500 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Kuna, Ewelina
Bocian, Aleksandra
Hus, Konrad K.
Petrilla, Vladimir
Petrillova, Monika
Legath, Jaroslav
Lewinska, Anna
Wnuk, Maciej
Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title_full Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title_fullStr Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title_full_unstemmed Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title_short Evaluation of Antifungal Activity of Naja pallida and Naja mossambica Venoms against Three Candida Species
title_sort evaluation of antifungal activity of naja pallida and naja mossambica venoms against three candida species
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472363/
https://www.ncbi.nlm.nih.gov/pubmed/32759763
http://dx.doi.org/10.3390/toxins12080500
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