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Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
[Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472746/ https://www.ncbi.nlm.nih.gov/pubmed/32872786 http://dx.doi.org/10.1021/jasms.0c00070 |
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author | Cao, Jianhua Goossens, Pieter Martin-Lorenzo, Marta Dewez, Frédéric Claes, Britt S. R. Biessen, Erik A. L. Heeren, Ron M. A. Balluff, Benjamin |
author_facet | Cao, Jianhua Goossens, Pieter Martin-Lorenzo, Marta Dewez, Frédéric Claes, Britt S. R. Biessen, Erik A. L. Heeren, Ron M. A. Balluff, Benjamin |
author_sort | Cao, Jianhua |
collection | PubMed |
description | [Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution of the involved lipids have not been thoroughly mapped yet. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) enables analyzing and visualizing hundreds of lipid molecules within the plaque while preserving each lipid’s specific location. In this study, we aim to identify and verify aortic plaque-specific lipids with high-spatial-resolution 2D and 3D MALDI-MSI common to high-fat-diet-fed low-density lipoprotein receptor deficient (ldlr(–/–)) mice and chow-fed apolipoprotein E deficient (apoe(–/–)) mice, the two most widely used animal models for atherosclerosis. A total of 11 lipids were found to be significantly and specifically colocalized to the plaques in both mouse models. These were identified and belong to one sphingomyelin (SM), three lysophosphatidic acids (LPA), four lysophosphatidylcholines (LPC), two lysophosphatidylethanolamines (LPE), and one lysophosphatidylinositol (LPI). While these lysolipids and SM 34:0;2 were characteristic of the atherosclerotic aorta plaque itself, LPI 18:0 was mainly localized in the necrotic core of the plaque. |
format | Online Article Text |
id | pubmed-7472746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74727462020-09-08 Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Cao, Jianhua Goossens, Pieter Martin-Lorenzo, Marta Dewez, Frédéric Claes, Britt S. R. Biessen, Erik A. L. Heeren, Ron M. A. Balluff, Benjamin J Am Soc Mass Spectrom [Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution of the involved lipids have not been thoroughly mapped yet. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) enables analyzing and visualizing hundreds of lipid molecules within the plaque while preserving each lipid’s specific location. In this study, we aim to identify and verify aortic plaque-specific lipids with high-spatial-resolution 2D and 3D MALDI-MSI common to high-fat-diet-fed low-density lipoprotein receptor deficient (ldlr(–/–)) mice and chow-fed apolipoprotein E deficient (apoe(–/–)) mice, the two most widely used animal models for atherosclerosis. A total of 11 lipids were found to be significantly and specifically colocalized to the plaques in both mouse models. These were identified and belong to one sphingomyelin (SM), three lysophosphatidic acids (LPA), four lysophosphatidylcholines (LPC), two lysophosphatidylethanolamines (LPE), and one lysophosphatidylinositol (LPI). While these lysolipids and SM 34:0;2 were characteristic of the atherosclerotic aorta plaque itself, LPI 18:0 was mainly localized in the necrotic core of the plaque. American Chemical Society 2020-07-27 2020-09-02 /pmc/articles/PMC7472746/ /pubmed/32872786 http://dx.doi.org/10.1021/jasms.0c00070 Text en Published by the American Chemical Society. All rights reserved. This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Cao, Jianhua Goossens, Pieter Martin-Lorenzo, Marta Dewez, Frédéric Claes, Britt S. R. Biessen, Erik A. L. Heeren, Ron M. A. Balluff, Benjamin Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging |
title | Atheroma-Specific Lipids in ldlr(–/–)
and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry Imaging |
title_full | Atheroma-Specific Lipids in ldlr(–/–)
and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry Imaging |
title_fullStr | Atheroma-Specific Lipids in ldlr(–/–)
and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry Imaging |
title_full_unstemmed | Atheroma-Specific Lipids in ldlr(–/–)
and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry Imaging |
title_short | Atheroma-Specific Lipids in ldlr(–/–)
and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted
Laser Desorption/Ionization Mass Spectrometry Imaging |
title_sort | atheroma-specific lipids in ldlr(–/–)
and apoe(–/–) mice using 2d and 3d matrix-assisted
laser desorption/ionization mass spectrometry imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472746/ https://www.ncbi.nlm.nih.gov/pubmed/32872786 http://dx.doi.org/10.1021/jasms.0c00070 |
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