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Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

[Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution...

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Autores principales: Cao, Jianhua, Goossens, Pieter, Martin-Lorenzo, Marta, Dewez, Frédéric, Claes, Britt S. R., Biessen, Erik A. L., Heeren, Ron M. A., Balluff, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472746/
https://www.ncbi.nlm.nih.gov/pubmed/32872786
http://dx.doi.org/10.1021/jasms.0c00070
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author Cao, Jianhua
Goossens, Pieter
Martin-Lorenzo, Marta
Dewez, Frédéric
Claes, Britt S. R.
Biessen, Erik A. L.
Heeren, Ron M. A.
Balluff, Benjamin
author_facet Cao, Jianhua
Goossens, Pieter
Martin-Lorenzo, Marta
Dewez, Frédéric
Claes, Britt S. R.
Biessen, Erik A. L.
Heeren, Ron M. A.
Balluff, Benjamin
author_sort Cao, Jianhua
collection PubMed
description [Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution of the involved lipids have not been thoroughly mapped yet. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) enables analyzing and visualizing hundreds of lipid molecules within the plaque while preserving each lipid’s specific location. In this study, we aim to identify and verify aortic plaque-specific lipids with high-spatial-resolution 2D and 3D MALDI-MSI common to high-fat-diet-fed low-density lipoprotein receptor deficient (ldlr(–/–)) mice and chow-fed apolipoprotein E deficient (apoe(–/–)) mice, the two most widely used animal models for atherosclerosis. A total of 11 lipids were found to be significantly and specifically colocalized to the plaques in both mouse models. These were identified and belong to one sphingomyelin (SM), three lysophosphatidic acids (LPA), four lysophosphatidylcholines (LPC), two lysophosphatidylethanolamines (LPE), and one lysophosphatidylinositol (LPI). While these lysolipids and SM 34:0;2 were characteristic of the atherosclerotic aorta plaque itself, LPI 18:0 was mainly localized in the necrotic core of the plaque.
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spelling pubmed-74727462020-09-08 Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Cao, Jianhua Goossens, Pieter Martin-Lorenzo, Marta Dewez, Frédéric Claes, Britt S. R. Biessen, Erik A. L. Heeren, Ron M. A. Balluff, Benjamin J Am Soc Mass Spectrom [Image: see text] Atherosclerosis is the major contributor to cardiovascular diseases. It is a spatially and temporally complex inflammatory disease, in which intravascular accumulation of a plethora of lipids is considered to play a crucial role. To date, both the composition and local distribution of the involved lipids have not been thoroughly mapped yet. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) enables analyzing and visualizing hundreds of lipid molecules within the plaque while preserving each lipid’s specific location. In this study, we aim to identify and verify aortic plaque-specific lipids with high-spatial-resolution 2D and 3D MALDI-MSI common to high-fat-diet-fed low-density lipoprotein receptor deficient (ldlr(–/–)) mice and chow-fed apolipoprotein E deficient (apoe(–/–)) mice, the two most widely used animal models for atherosclerosis. A total of 11 lipids were found to be significantly and specifically colocalized to the plaques in both mouse models. These were identified and belong to one sphingomyelin (SM), three lysophosphatidic acids (LPA), four lysophosphatidylcholines (LPC), two lysophosphatidylethanolamines (LPE), and one lysophosphatidylinositol (LPI). While these lysolipids and SM 34:0;2 were characteristic of the atherosclerotic aorta plaque itself, LPI 18:0 was mainly localized in the necrotic core of the plaque. American Chemical Society 2020-07-27 2020-09-02 /pmc/articles/PMC7472746/ /pubmed/32872786 http://dx.doi.org/10.1021/jasms.0c00070 Text en Published by the American Chemical Society. All rights reserved. This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Cao, Jianhua
Goossens, Pieter
Martin-Lorenzo, Marta
Dewez, Frédéric
Claes, Britt S. R.
Biessen, Erik A. L.
Heeren, Ron M. A.
Balluff, Benjamin
Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title_full Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title_fullStr Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title_full_unstemmed Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title_short Atheroma-Specific Lipids in ldlr(–/–) and apoe(–/–) Mice Using 2D and 3D Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
title_sort atheroma-specific lipids in ldlr(–/–) and apoe(–/–) mice using 2d and 3d matrix-assisted laser desorption/ionization mass spectrometry imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472746/
https://www.ncbi.nlm.nih.gov/pubmed/32872786
http://dx.doi.org/10.1021/jasms.0c00070
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