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Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin

The long-term effects of palmitate (PA) on osteogenic differentiation capacity of human mesenchymal stromal cells (hMSCs) were investigated by cultivating the cells in osteogenic differentiation medium (O-w/o) and osteogenic medium containing PA (O-BSA-PA) for 21 days. Osteogenic medium containing B...

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Autores principales: Glenske, Kristina, Schäpe, Kaija, Wieck, Anneke, Failing, Klaus, Werner, Janina, Rohnke, Marcus, Wenisch, Sabine, Mazurek, Sybille
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472858/
https://www.ncbi.nlm.nih.gov/pubmed/32913884
http://dx.doi.org/10.1016/j.bonr.2020.100707
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author Glenske, Kristina
Schäpe, Kaija
Wieck, Anneke
Failing, Klaus
Werner, Janina
Rohnke, Marcus
Wenisch, Sabine
Mazurek, Sybille
author_facet Glenske, Kristina
Schäpe, Kaija
Wieck, Anneke
Failing, Klaus
Werner, Janina
Rohnke, Marcus
Wenisch, Sabine
Mazurek, Sybille
author_sort Glenske, Kristina
collection PubMed
description The long-term effects of palmitate (PA) on osteogenic differentiation capacity of human mesenchymal stromal cells (hMSCs) were investigated by cultivating the cells in osteogenic differentiation medium (O-w/o) and osteogenic medium containing PA (O-BSA-PA) for 21 days. Osteogenic medium containing BSA (O-BSA) was used as a control. By means of rt-qPCR, successful osteogenic differentiation was observed in the O-w/o regarding the levels of osteogenic and cell-communication related genes (OCN, Col1, BMP2, ITGA1, ITGB1, Cx43, sp1) in contrast to expression levels observed in cells incubated within basal medium. However, in the O-BSA, these genes were found to have decreased significantly. In cases of Cx43 and sp1, PA significantly reinforced the reductive effect of BSA alone. O-BSA notably decreased glucose and pyruvate consumption, whereas glutamine consumption significantly increased. In comparison to O-BSA addition of PA significantly reduced glycolysis and glutaminolysis. ToF-SIMS analysis confirmed increased incorporation of supplemented deuterated PA into the cell membranes, while the overall PA-concentration remained unchanged compared to cells incubated in the O-BSA and O-w/o. Therefore, the effects on gene expression and the metabolism did not result from the membrane alterations, but may have risen from inter- and intracellular effects brought on by BSA and PA.
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spelling pubmed-74728582020-09-09 Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin Glenske, Kristina Schäpe, Kaija Wieck, Anneke Failing, Klaus Werner, Janina Rohnke, Marcus Wenisch, Sabine Mazurek, Sybille Bone Rep Article The long-term effects of palmitate (PA) on osteogenic differentiation capacity of human mesenchymal stromal cells (hMSCs) were investigated by cultivating the cells in osteogenic differentiation medium (O-w/o) and osteogenic medium containing PA (O-BSA-PA) for 21 days. Osteogenic medium containing BSA (O-BSA) was used as a control. By means of rt-qPCR, successful osteogenic differentiation was observed in the O-w/o regarding the levels of osteogenic and cell-communication related genes (OCN, Col1, BMP2, ITGA1, ITGB1, Cx43, sp1) in contrast to expression levels observed in cells incubated within basal medium. However, in the O-BSA, these genes were found to have decreased significantly. In cases of Cx43 and sp1, PA significantly reinforced the reductive effect of BSA alone. O-BSA notably decreased glucose and pyruvate consumption, whereas glutamine consumption significantly increased. In comparison to O-BSA addition of PA significantly reduced glycolysis and glutaminolysis. ToF-SIMS analysis confirmed increased incorporation of supplemented deuterated PA into the cell membranes, while the overall PA-concentration remained unchanged compared to cells incubated in the O-BSA and O-w/o. Therefore, the effects on gene expression and the metabolism did not result from the membrane alterations, but may have risen from inter- and intracellular effects brought on by BSA and PA. Elsevier 2020-08-13 /pmc/articles/PMC7472858/ /pubmed/32913884 http://dx.doi.org/10.1016/j.bonr.2020.100707 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Glenske, Kristina
Schäpe, Kaija
Wieck, Anneke
Failing, Klaus
Werner, Janina
Rohnke, Marcus
Wenisch, Sabine
Mazurek, Sybille
Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title_full Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title_fullStr Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title_full_unstemmed Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title_short Effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - Impact of albumin
title_sort effect of long term palmitate treatment on osteogenic differentiation of human mesenchymal stromal cells - impact of albumin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7472858/
https://www.ncbi.nlm.nih.gov/pubmed/32913884
http://dx.doi.org/10.1016/j.bonr.2020.100707
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