Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods

Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRI...

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Detalles Bibliográficos
Autores principales: Wang, Xinjie, Shang, Xiaoyun, Huang, Xingxu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473117/
https://www.ncbi.nlm.nih.gov/pubmed/32643563
http://dx.doi.org/10.1080/22221751.2020.1793689
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author Wang, Xinjie
Shang, Xiaoyun
Huang, Xingxu
author_facet Wang, Xinjie
Shang, Xiaoyun
Huang, Xingxu
author_sort Wang, Xinjie
collection PubMed
description Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.
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spelling pubmed-74731172020-09-15 Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods Wang, Xinjie Shang, Xiaoyun Huang, Xingxu Emerg Microbes Infect Review Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises. Taylor & Francis 2020-07-20 /pmc/articles/PMC7473117/ /pubmed/32643563 http://dx.doi.org/10.1080/22221751.2020.1793689 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Wang, Xinjie
Shang, Xiaoyun
Huang, Xingxu
Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title_full Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title_fullStr Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title_full_unstemmed Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title_short Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
title_sort next-generation pathogen diagnosis with crispr/cas-based detection methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473117/
https://www.ncbi.nlm.nih.gov/pubmed/32643563
http://dx.doi.org/10.1080/22221751.2020.1793689
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