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A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice
Influenza B virus (IBV) is one of the most important human respiratory viruses: it causes approximately one-third of the global influenza-related disease burden each year. However, compared with the several pathogenicity-related molecular markers that have been identified for influenza A virus (IAV)...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473139/ https://www.ncbi.nlm.nih.gov/pubmed/32746754 http://dx.doi.org/10.1080/22221751.2020.1806005 |
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author | Zhou, Lijuan Feng, Zhaomin Liu, Jia Chen, Yongkun Yang, Lei Liu, Suli Li, Xiyan Gao, Rongbao Zhu, Wenfei Wang, Dayan Shu, Yuelong |
author_facet | Zhou, Lijuan Feng, Zhaomin Liu, Jia Chen, Yongkun Yang, Lei Liu, Suli Li, Xiyan Gao, Rongbao Zhu, Wenfei Wang, Dayan Shu, Yuelong |
author_sort | Zhou, Lijuan |
collection | PubMed |
description | Influenza B virus (IBV) is one of the most important human respiratory viruses: it causes approximately one-third of the global influenza-related disease burden each year. However, compared with the several pathogenicity-related molecular markers that have been identified for influenza A virus (IAV), little is known about potential IBV pathogenicity-related markers. Here, although the IBV strain B/Anhui-Tunxi/1528/2014 (AH1528/14) exhibited a more efficient replication ability in vitro and higher pathogenicity in vivo compared with IBV strain B/Anhui-Baohe/127/2015 (AH127/15), only three amino acids differences (HA(A390E), NA(N342D) and PB1(V212I)) were observed among their full genomes. The contributions of each amino acid difference to the virus pathogenicity were further investigated. Compared with the wild type IBV virus rAH127, the recombinant virus harbouring a single substitution of HA(A390E) had a similar phenotype, whereas the recombinant virus harbouring PB1(V212I) replicated to a moderately higher titre in both MDCK cells and in mice. Notably, the virus harbouring NA(N342D) showed significantly better growth properties in MDCK cells and higher fatality rates in mice. In addition, the presence of NA(N342D) dramatically enhanced the viral neuraminidase activity. In conclusion, our study identified a novel IBV molecular marker, NA(N342D), that could significantly increase the virulence of IBV in mice. |
format | Online Article Text |
id | pubmed-7473139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-74731392020-09-15 A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice Zhou, Lijuan Feng, Zhaomin Liu, Jia Chen, Yongkun Yang, Lei Liu, Suli Li, Xiyan Gao, Rongbao Zhu, Wenfei Wang, Dayan Shu, Yuelong Emerg Microbes Infect Articles Influenza B virus (IBV) is one of the most important human respiratory viruses: it causes approximately one-third of the global influenza-related disease burden each year. However, compared with the several pathogenicity-related molecular markers that have been identified for influenza A virus (IAV), little is known about potential IBV pathogenicity-related markers. Here, although the IBV strain B/Anhui-Tunxi/1528/2014 (AH1528/14) exhibited a more efficient replication ability in vitro and higher pathogenicity in vivo compared with IBV strain B/Anhui-Baohe/127/2015 (AH127/15), only three amino acids differences (HA(A390E), NA(N342D) and PB1(V212I)) were observed among their full genomes. The contributions of each amino acid difference to the virus pathogenicity were further investigated. Compared with the wild type IBV virus rAH127, the recombinant virus harbouring a single substitution of HA(A390E) had a similar phenotype, whereas the recombinant virus harbouring PB1(V212I) replicated to a moderately higher titre in both MDCK cells and in mice. Notably, the virus harbouring NA(N342D) showed significantly better growth properties in MDCK cells and higher fatality rates in mice. In addition, the presence of NA(N342D) dramatically enhanced the viral neuraminidase activity. In conclusion, our study identified a novel IBV molecular marker, NA(N342D), that could significantly increase the virulence of IBV in mice. Taylor & Francis 2020-08-21 /pmc/articles/PMC7473139/ /pubmed/32746754 http://dx.doi.org/10.1080/22221751.2020.1806005 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Zhou, Lijuan Feng, Zhaomin Liu, Jia Chen, Yongkun Yang, Lei Liu, Suli Li, Xiyan Gao, Rongbao Zhu, Wenfei Wang, Dayan Shu, Yuelong A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title | A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title_full | A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title_fullStr | A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title_full_unstemmed | A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title_short | A single N342D substitution in Influenza B Virus NA protein determines viral pathogenicity in mice |
title_sort | single n342d substitution in influenza b virus na protein determines viral pathogenicity in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473139/ https://www.ncbi.nlm.nih.gov/pubmed/32746754 http://dx.doi.org/10.1080/22221751.2020.1806005 |
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