Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing

The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

Detalles Bibliográficos
Autores principales: Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Errico, Giulia, Iacobino, Angelo, Bella, Antonino, Riccardo, Flavia, Marsili, Giulia, Stefanelli, Paola, Pezzotti, Patrizio, Rezza, Giovanni, Ciervo, Alessandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473159/
https://www.ncbi.nlm.nih.gov/pubmed/32552549
http://dx.doi.org/10.1080/22221751.2020.1775500
Descripción
Sumario:The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.

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