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Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing
The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Taylor & Francis
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473159/ https://www.ncbi.nlm.nih.gov/pubmed/32552549 http://dx.doi.org/10.1080/22221751.2020.1775500 |
| _version_ | 1783579129588744192 |
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| author | Mancini, Fabiola Barbanti, Fabrizio Scaturro, Maria Errico, Giulia Iacobino, Angelo Bella, Antonino Riccardo, Flavia Marsili, Giulia Stefanelli, Paola Pezzotti, Patrizio Rezza, Giovanni Ciervo, Alessandra |
| author_facet | Mancini, Fabiola Barbanti, Fabrizio Scaturro, Maria Errico, Giulia Iacobino, Angelo Bella, Antonino Riccardo, Flavia Marsili, Giulia Stefanelli, Paola Pezzotti, Patrizio Rezza, Giovanni Ciervo, Alessandra |
| author_sort | Mancini, Fabiola |
| collection | PubMed |
| description | The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification. |
| format | Online Article Text |
| id | pubmed-7473159 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Taylor & Francis |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-74731592020-09-15 Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing Mancini, Fabiola Barbanti, Fabrizio Scaturro, Maria Errico, Giulia Iacobino, Angelo Bella, Antonino Riccardo, Flavia Marsili, Giulia Stefanelli, Paola Pezzotti, Patrizio Rezza, Giovanni Ciervo, Alessandra Emerg Microbes Infect Letter The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification. Taylor & Francis 2020-06-18 /pmc/articles/PMC7473159/ /pubmed/32552549 http://dx.doi.org/10.1080/22221751.2020.1775500 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Letter Mancini, Fabiola Barbanti, Fabrizio Scaturro, Maria Errico, Giulia Iacobino, Angelo Bella, Antonino Riccardo, Flavia Marsili, Giulia Stefanelli, Paola Pezzotti, Patrizio Rezza, Giovanni Ciervo, Alessandra Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title | Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title_full | Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title_fullStr | Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title_full_unstemmed | Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title_short | Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing |
| title_sort | laboratory management for sars-cov-2 detection: a user-friendly combination of the heat treatment approach and rt-real-time pcr testing |
| topic | Letter |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473159/ https://www.ncbi.nlm.nih.gov/pubmed/32552549 http://dx.doi.org/10.1080/22221751.2020.1775500 |
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