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Revealing the in vivo growth and division patterns of mouse gut bacteria
Current techniques for studying gut microbiota are unable to answer some important microbiology questions, like how different bacteria grow and divide in the gut. We propose a method that integrates the use of sequential d-amino acid–based in vivo metabolic labeling with fluorescence in situ hybridi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473744/ https://www.ncbi.nlm.nih.gov/pubmed/32917613 http://dx.doi.org/10.1126/sciadv.abb2531 |
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author | Lin, Liyuan Wu, Qiuyue Song, Jia Du, Yahui Gao, Juan Song, Yanling Wang, Wei Yang, Chaoyong |
author_facet | Lin, Liyuan Wu, Qiuyue Song, Jia Du, Yahui Gao, Juan Song, Yanling Wang, Wei Yang, Chaoyong |
author_sort | Lin, Liyuan |
collection | PubMed |
description | Current techniques for studying gut microbiota are unable to answer some important microbiology questions, like how different bacteria grow and divide in the gut. We propose a method that integrates the use of sequential d-amino acid–based in vivo metabolic labeling with fluorescence in situ hybridization (FISH), for characterizing the growth and division patterns of gut bacteria. After sequentially administering two d-amino acid–based probes containing different fluorophores to mice by gavage, the resulting dual-labeled peptidoglycans provide temporal information on cell wall synthesis of gut bacteria. Following taxonomic identification with FISH probes, the growth and division patterns of the corresponding bacterial taxa, including species that cannot be cultured separately in vitro, are revealed. Our method offers a facile yet powerful tool for investigating the in vivo growth dynamics of the bacterial gut microbiota, which will advance our understanding of bacterial cytology and facilitate elucidation of the basic microbiology of this gut “dark matter.” |
format | Online Article Text |
id | pubmed-7473744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-74737442020-09-17 Revealing the in vivo growth and division patterns of mouse gut bacteria Lin, Liyuan Wu, Qiuyue Song, Jia Du, Yahui Gao, Juan Song, Yanling Wang, Wei Yang, Chaoyong Sci Adv Research Articles Current techniques for studying gut microbiota are unable to answer some important microbiology questions, like how different bacteria grow and divide in the gut. We propose a method that integrates the use of sequential d-amino acid–based in vivo metabolic labeling with fluorescence in situ hybridization (FISH), for characterizing the growth and division patterns of gut bacteria. After sequentially administering two d-amino acid–based probes containing different fluorophores to mice by gavage, the resulting dual-labeled peptidoglycans provide temporal information on cell wall synthesis of gut bacteria. Following taxonomic identification with FISH probes, the growth and division patterns of the corresponding bacterial taxa, including species that cannot be cultured separately in vitro, are revealed. Our method offers a facile yet powerful tool for investigating the in vivo growth dynamics of the bacterial gut microbiota, which will advance our understanding of bacterial cytology and facilitate elucidation of the basic microbiology of this gut “dark matter.” American Association for the Advancement of Science 2020-09-04 /pmc/articles/PMC7473744/ /pubmed/32917613 http://dx.doi.org/10.1126/sciadv.abb2531 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Lin, Liyuan Wu, Qiuyue Song, Jia Du, Yahui Gao, Juan Song, Yanling Wang, Wei Yang, Chaoyong Revealing the in vivo growth and division patterns of mouse gut bacteria |
title | Revealing the in vivo growth and division patterns of mouse gut bacteria |
title_full | Revealing the in vivo growth and division patterns of mouse gut bacteria |
title_fullStr | Revealing the in vivo growth and division patterns of mouse gut bacteria |
title_full_unstemmed | Revealing the in vivo growth and division patterns of mouse gut bacteria |
title_short | Revealing the in vivo growth and division patterns of mouse gut bacteria |
title_sort | revealing the in vivo growth and division patterns of mouse gut bacteria |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473744/ https://www.ncbi.nlm.nih.gov/pubmed/32917613 http://dx.doi.org/10.1126/sciadv.abb2531 |
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