The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes

The conspicuous bright golden to orange-reddish coloration of species of the basidiomycete genus Laetiporus is a hallmark feature of their fruiting bodies, known among mushroom hunters as the “chicken of the woods”. This report describes the identification of an eight-domain mono-modular highly redu...

Descripción completa

Detalles Bibliográficos
Autores principales: Seibold, Paula Sophie, Lenz, Claudius, Gressler, Markus, Hoffmeister, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Special Feature: Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473843/
https://www.ncbi.nlm.nih.gov/pubmed/32820242
http://dx.doi.org/10.1038/s41429-020-00362-6
_version_ 1783579246400110592
author Seibold, Paula Sophie
Lenz, Claudius
Gressler, Markus
Hoffmeister, Dirk
author_facet Seibold, Paula Sophie
Lenz, Claudius
Gressler, Markus
Hoffmeister, Dirk
author_sort Seibold, Paula Sophie
collection PubMed
description The conspicuous bright golden to orange-reddish coloration of species of the basidiomycete genus Laetiporus is a hallmark feature of their fruiting bodies, known among mushroom hunters as the “chicken of the woods”. This report describes the identification of an eight-domain mono-modular highly reducing polyketide synthase as sole enzyme necessary for laetiporic acid biosynthesis. Heterologous pathway reconstitution in both Aspergillus nidulans and Aspergillus niger verified that LpaA functions as a multi-chain length polyene synthase, which produces a cocktail of laetiporic acids with a methyl-branched C(26)–C(32) main chain. Laetiporic acids show a marked antifungal activity on Aspergillus protoplasts. Given the multiple products of a single biosynthesis enzyme, our work underscores the diversity-oriented character of basidiomycete natural product biosynthesis.
format Online
Article
Text
id pubmed-7473843
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-74738432020-09-16 The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes Seibold, Paula Sophie Lenz, Claudius Gressler, Markus Hoffmeister, Dirk J Antibiot (Tokyo) Special Feature: Article The conspicuous bright golden to orange-reddish coloration of species of the basidiomycete genus Laetiporus is a hallmark feature of their fruiting bodies, known among mushroom hunters as the “chicken of the woods”. This report describes the identification of an eight-domain mono-modular highly reducing polyketide synthase as sole enzyme necessary for laetiporic acid biosynthesis. Heterologous pathway reconstitution in both Aspergillus nidulans and Aspergillus niger verified that LpaA functions as a multi-chain length polyene synthase, which produces a cocktail of laetiporic acids with a methyl-branched C(26)–C(32) main chain. Laetiporic acids show a marked antifungal activity on Aspergillus protoplasts. Given the multiple products of a single biosynthesis enzyme, our work underscores the diversity-oriented character of basidiomycete natural product biosynthesis. Nature Publishing Group UK 2020-08-21 2020 /pmc/articles/PMC7473843/ /pubmed/32820242 http://dx.doi.org/10.1038/s41429-020-00362-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Special Feature: Article
Seibold, Paula Sophie
Lenz, Claudius
Gressler, Markus
Hoffmeister, Dirk
The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title_full The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title_fullStr The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title_full_unstemmed The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title_short The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes
title_sort laetiporus polyketide synthase lpaa produces a series of antifungal polyenes
topic Special Feature: Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473843/
https://www.ncbi.nlm.nih.gov/pubmed/32820242
http://dx.doi.org/10.1038/s41429-020-00362-6
work_keys_str_mv AT seiboldpaulasophie thelaetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT lenzclaudius thelaetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT gresslermarkus thelaetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT hoffmeisterdirk thelaetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT seiboldpaulasophie laetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT lenzclaudius laetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT gresslermarkus laetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes
AT hoffmeisterdirk laetiporuspolyketidesynthaselpaaproducesaseriesofantifungalpolyenes

Ejemplares similares