Cargando…
THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down,...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474744/ https://www.ncbi.nlm.nih.gov/pubmed/32908912 http://dx.doi.org/10.1155/2020/7838924 |
_version_ | 1783579377133420544 |
---|---|
author | Zhang, Jing Zhang, Huahua Shi, Haiyan Wang, Fenghui Du, Juan Wang, Yu Wei, Yameng Xue, Wanjuan Li, Dan Feng, Yun Yan, Jing Gao, Yi Li, Jia Han, Jiming |
author_facet | Zhang, Jing Zhang, Huahua Shi, Haiyan Wang, Fenghui Du, Juan Wang, Yu Wei, Yameng Xue, Wanjuan Li, Dan Feng, Yun Yan, Jing Gao, Yi Li, Jia Han, Jiming |
author_sort | Zhang, Jing |
collection | PubMed |
description | We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down, cell proliferation, cell cycle, and apoptosis were detected in MKN-45 cells. We found that THAP11 was significantly downregulated in GC tissues and GC cell lines. Functionally, THAP11 overexpression markedly inhibited cell growth, induced G1/G0 cell-cycle arrest, and promoted cell apoptosis of MKN-45 cells, while silencing of THAP11 led to increased cell growth, increased DNA synthesis, and inhibited apoptosis. In addition, THAP11 negatively regulated the expression of c-Myc, decreased cyclinD1 protein, and increased p27 and p21 protein levels. We also found cell growth suppression induced by THAP11 was rescued by c-Myc overexpression, further confirming that THAP11 suppresses gastric cancer cell growth via the c-Myc pathway. THAP11 acts as a cell growth suppressor and exerts its role possibly through negatively regulating c-Myc pathway in gastric cancer. |
format | Online Article Text |
id | pubmed-7474744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-74747442020-09-08 THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways Zhang, Jing Zhang, Huahua Shi, Haiyan Wang, Fenghui Du, Juan Wang, Yu Wei, Yameng Xue, Wanjuan Li, Dan Feng, Yun Yan, Jing Gao, Yi Li, Jia Han, Jiming Biomed Res Int Research Article We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down, cell proliferation, cell cycle, and apoptosis were detected in MKN-45 cells. We found that THAP11 was significantly downregulated in GC tissues and GC cell lines. Functionally, THAP11 overexpression markedly inhibited cell growth, induced G1/G0 cell-cycle arrest, and promoted cell apoptosis of MKN-45 cells, while silencing of THAP11 led to increased cell growth, increased DNA synthesis, and inhibited apoptosis. In addition, THAP11 negatively regulated the expression of c-Myc, decreased cyclinD1 protein, and increased p27 and p21 protein levels. We also found cell growth suppression induced by THAP11 was rescued by c-Myc overexpression, further confirming that THAP11 suppresses gastric cancer cell growth via the c-Myc pathway. THAP11 acts as a cell growth suppressor and exerts its role possibly through negatively regulating c-Myc pathway in gastric cancer. Hindawi 2020-08-27 /pmc/articles/PMC7474744/ /pubmed/32908912 http://dx.doi.org/10.1155/2020/7838924 Text en Copyright © 2020 Jing Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Jing Zhang, Huahua Shi, Haiyan Wang, Fenghui Du, Juan Wang, Yu Wei, Yameng Xue, Wanjuan Li, Dan Feng, Yun Yan, Jing Gao, Yi Li, Jia Han, Jiming THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title | THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title_full | THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title_fullStr | THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title_full_unstemmed | THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title_short | THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways |
title_sort | thap11 functions as a tumor suppressor in gastric cancer through regulating c-myc signaling pathways |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474744/ https://www.ncbi.nlm.nih.gov/pubmed/32908912 http://dx.doi.org/10.1155/2020/7838924 |
work_keys_str_mv | AT zhangjing thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT zhanghuahua thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT shihaiyan thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT wangfenghui thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT dujuan thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT wangyu thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT weiyameng thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT xuewanjuan thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT lidan thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT fengyun thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT yanjing thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT gaoyi thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT lijia thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways AT hanjiming thap11functionsasatumorsuppressoringastriccancerthroughregulatingcmycsignalingpathways |