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THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways

We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down,...

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Autores principales: Zhang, Jing, Zhang, Huahua, Shi, Haiyan, Wang, Fenghui, Du, Juan, Wang, Yu, Wei, Yameng, Xue, Wanjuan, Li, Dan, Feng, Yun, Yan, Jing, Gao, Yi, Li, Jia, Han, Jiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474744/
https://www.ncbi.nlm.nih.gov/pubmed/32908912
http://dx.doi.org/10.1155/2020/7838924
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author Zhang, Jing
Zhang, Huahua
Shi, Haiyan
Wang, Fenghui
Du, Juan
Wang, Yu
Wei, Yameng
Xue, Wanjuan
Li, Dan
Feng, Yun
Yan, Jing
Gao, Yi
Li, Jia
Han, Jiming
author_facet Zhang, Jing
Zhang, Huahua
Shi, Haiyan
Wang, Fenghui
Du, Juan
Wang, Yu
Wei, Yameng
Xue, Wanjuan
Li, Dan
Feng, Yun
Yan, Jing
Gao, Yi
Li, Jia
Han, Jiming
author_sort Zhang, Jing
collection PubMed
description We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down, cell proliferation, cell cycle, and apoptosis were detected in MKN-45 cells. We found that THAP11 was significantly downregulated in GC tissues and GC cell lines. Functionally, THAP11 overexpression markedly inhibited cell growth, induced G1/G0 cell-cycle arrest, and promoted cell apoptosis of MKN-45 cells, while silencing of THAP11 led to increased cell growth, increased DNA synthesis, and inhibited apoptosis. In addition, THAP11 negatively regulated the expression of c-Myc, decreased cyclinD1 protein, and increased p27 and p21 protein levels. We also found cell growth suppression induced by THAP11 was rescued by c-Myc overexpression, further confirming that THAP11 suppresses gastric cancer cell growth via the c-Myc pathway. THAP11 acts as a cell growth suppressor and exerts its role possibly through negatively regulating c-Myc pathway in gastric cancer.
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spelling pubmed-74747442020-09-08 THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways Zhang, Jing Zhang, Huahua Shi, Haiyan Wang, Fenghui Du, Juan Wang, Yu Wei, Yameng Xue, Wanjuan Li, Dan Feng, Yun Yan, Jing Gao, Yi Li, Jia Han, Jiming Biomed Res Int Research Article We aim to investigate the role of THAP11 (thanatos-associated protein11) in gastric cancer and its regulation mechanisms. THAP11 expression was analyzed in 51 pairs of GC tissues and the corresponding paracancerous tissues by qRT-PCR and Western blot. After THAP11 was overexpressed or knocked-down, cell proliferation, cell cycle, and apoptosis were detected in MKN-45 cells. We found that THAP11 was significantly downregulated in GC tissues and GC cell lines. Functionally, THAP11 overexpression markedly inhibited cell growth, induced G1/G0 cell-cycle arrest, and promoted cell apoptosis of MKN-45 cells, while silencing of THAP11 led to increased cell growth, increased DNA synthesis, and inhibited apoptosis. In addition, THAP11 negatively regulated the expression of c-Myc, decreased cyclinD1 protein, and increased p27 and p21 protein levels. We also found cell growth suppression induced by THAP11 was rescued by c-Myc overexpression, further confirming that THAP11 suppresses gastric cancer cell growth via the c-Myc pathway. THAP11 acts as a cell growth suppressor and exerts its role possibly through negatively regulating c-Myc pathway in gastric cancer. Hindawi 2020-08-27 /pmc/articles/PMC7474744/ /pubmed/32908912 http://dx.doi.org/10.1155/2020/7838924 Text en Copyright © 2020 Jing Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Jing
Zhang, Huahua
Shi, Haiyan
Wang, Fenghui
Du, Juan
Wang, Yu
Wei, Yameng
Xue, Wanjuan
Li, Dan
Feng, Yun
Yan, Jing
Gao, Yi
Li, Jia
Han, Jiming
THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title_full THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title_fullStr THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title_full_unstemmed THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title_short THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways
title_sort thap11 functions as a tumor suppressor in gastric cancer through regulating c-myc signaling pathways
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474744/
https://www.ncbi.nlm.nih.gov/pubmed/32908912
http://dx.doi.org/10.1155/2020/7838924
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