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Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple

Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple...

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Autores principales: An, Jian-Ping, Liu, Ya-Jing, Zhang, Xiao-Wei, Bi, Si-Qi, Wang, Xiao-Fei, You, Chun-Xiang, Hao, Yu-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475178/
https://www.ncbi.nlm.nih.gov/pubmed/31996900
http://dx.doi.org/10.1093/jxb/eraa056
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author An, Jian-Ping
Liu, Ya-Jing
Zhang, Xiao-Wei
Bi, Si-Qi
Wang, Xiao-Fei
You, Chun-Xiang
Hao, Yu-Jin
author_facet An, Jian-Ping
Liu, Ya-Jing
Zhang, Xiao-Wei
Bi, Si-Qi
Wang, Xiao-Fei
You, Chun-Xiang
Hao, Yu-Jin
author_sort An, Jian-Ping
collection PubMed
description Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple (Malus domestica) was found to be largely dependent on the functions of the MdMYB1 and MdTCP46 transcription factors. The expression of MdTCP46 was responsive to high light intensity, and under these conditions it promoted anthocyanin biosynthesis by direct interactions with MdMYB1 that enhanced the binding of the latter to its target genes. MdTCP46 also interacted with a bric-a-brac/tramtrack/broad (BTB) protein, MdBT2, that is responsive to high light intensity, which ubiquitinated MdTCP46 and mediated its degradation via the 26S proteasome pathway. Our results demonstrate that the dynamic regulatory module MdBT2-MdTCP46-MdMYB1 plays a key role in modulating anthocyanin biosynthesis at different light intensities in apple, and provides new insights into the post-transcriptional regulation of TCP proteins.
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spelling pubmed-74751782020-09-10 Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple An, Jian-Ping Liu, Ya-Jing Zhang, Xiao-Wei Bi, Si-Qi Wang, Xiao-Fei You, Chun-Xiang Hao, Yu-Jin J Exp Bot Research Papers Teosinte branched1/cycloidea/proliferating (TCP) transcription factors play a broad role in plant growth and development, but their involvement in the regulation of anthocyanin biosynthesis is currently unclear. In this study, anthocyanin biosynthesis induced by different light intensities in apple (Malus domestica) was found to be largely dependent on the functions of the MdMYB1 and MdTCP46 transcription factors. The expression of MdTCP46 was responsive to high light intensity, and under these conditions it promoted anthocyanin biosynthesis by direct interactions with MdMYB1 that enhanced the binding of the latter to its target genes. MdTCP46 also interacted with a bric-a-brac/tramtrack/broad (BTB) protein, MdBT2, that is responsive to high light intensity, which ubiquitinated MdTCP46 and mediated its degradation via the 26S proteasome pathway. Our results demonstrate that the dynamic regulatory module MdBT2-MdTCP46-MdMYB1 plays a key role in modulating anthocyanin biosynthesis at different light intensities in apple, and provides new insights into the post-transcriptional regulation of TCP proteins. Oxford University Press 2020-05-30 2020-01-30 /pmc/articles/PMC7475178/ /pubmed/31996900 http://dx.doi.org/10.1093/jxb/eraa056 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
An, Jian-Ping
Liu, Ya-Jing
Zhang, Xiao-Wei
Bi, Si-Qi
Wang, Xiao-Fei
You, Chun-Xiang
Hao, Yu-Jin
Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title_full Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title_fullStr Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title_full_unstemmed Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title_short Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple
title_sort dynamic regulation of anthocyanin biosynthesis at different light intensities by the bt2-tcp46-myb1 module in apple
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475178/
https://www.ncbi.nlm.nih.gov/pubmed/31996900
http://dx.doi.org/10.1093/jxb/eraa056
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