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Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit

Phytoene synthase 1 (PSY1) and capsanthin-capsorubin synthase (CCS) are two major genes responsible for fruit color variation in pepper (Capsicum spp.). However, the role of PSY2 remains unknown. We used a systemic approach to examine the genetic factors responsible for the yellow fruit color of C....

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Autores principales: Jang, So-Jeong, Jeong, Hyo-Bong, Jung, Ayoung, Kang, Min-Young, Kim, Suna, Ha, Sun-Hwa, Kwon, Jin-Kyung, Kang, Byoung-Cheorl
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475241/
https://www.ncbi.nlm.nih.gov/pubmed/32219321
http://dx.doi.org/10.1093/jxb/eraa155
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author Jang, So-Jeong
Jeong, Hyo-Bong
Jung, Ayoung
Kang, Min-Young
Kim, Suna
Ha, Sun-Hwa
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
author_facet Jang, So-Jeong
Jeong, Hyo-Bong
Jung, Ayoung
Kang, Min-Young
Kim, Suna
Ha, Sun-Hwa
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
author_sort Jang, So-Jeong
collection PubMed
description Phytoene synthase 1 (PSY1) and capsanthin-capsorubin synthase (CCS) are two major genes responsible for fruit color variation in pepper (Capsicum spp.). However, the role of PSY2 remains unknown. We used a systemic approach to examine the genetic factors responsible for the yellow fruit color of C. annuum ‘MicroPep Yellow’ (MY) and to determine the role of PSY2 in fruit color. We detected complete deletion of PSY1 and a retrotransposon insertion in CCS. Despite the loss of PSY1 and CCS function, both MY and mutant F(2) plants from a cross between MY and the ‘MicroPep Red’ (MR) accumulated basal levels of carotenoids, indicating that other PSY genes may complement the loss of PSY1. qRT-PCR analysis indicated that PSY2 was constitutively expressed in both MR and MY fruits, and a color complementation assay using Escherichia coli revealed that PSY2 was capable of biosynthesizing a carotenoid. Virus-induced gene silencing of PSY2 in MY resulted in white fruits. These findings indicate that PSY2 can compensate for the absence of PSY1 in pepper fruit, resulting in the yellow color of MY fruits.
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spelling pubmed-74752412020-09-10 Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit Jang, So-Jeong Jeong, Hyo-Bong Jung, Ayoung Kang, Min-Young Kim, Suna Ha, Sun-Hwa Kwon, Jin-Kyung Kang, Byoung-Cheorl J Exp Bot Research Papers Phytoene synthase 1 (PSY1) and capsanthin-capsorubin synthase (CCS) are two major genes responsible for fruit color variation in pepper (Capsicum spp.). However, the role of PSY2 remains unknown. We used a systemic approach to examine the genetic factors responsible for the yellow fruit color of C. annuum ‘MicroPep Yellow’ (MY) and to determine the role of PSY2 in fruit color. We detected complete deletion of PSY1 and a retrotransposon insertion in CCS. Despite the loss of PSY1 and CCS function, both MY and mutant F(2) plants from a cross between MY and the ‘MicroPep Red’ (MR) accumulated basal levels of carotenoids, indicating that other PSY genes may complement the loss of PSY1. qRT-PCR analysis indicated that PSY2 was constitutively expressed in both MR and MY fruits, and a color complementation assay using Escherichia coli revealed that PSY2 was capable of biosynthesizing a carotenoid. Virus-induced gene silencing of PSY2 in MY resulted in white fruits. These findings indicate that PSY2 can compensate for the absence of PSY1 in pepper fruit, resulting in the yellow color of MY fruits. Oxford University Press 2020-06-22 2020-03-27 /pmc/articles/PMC7475241/ /pubmed/32219321 http://dx.doi.org/10.1093/jxb/eraa155 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Jang, So-Jeong
Jeong, Hyo-Bong
Jung, Ayoung
Kang, Min-Young
Kim, Suna
Ha, Sun-Hwa
Kwon, Jin-Kyung
Kang, Byoung-Cheorl
Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title_full Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title_fullStr Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title_full_unstemmed Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title_short Phytoene synthase 2 can compensate for the absence of PSY1 in the control of color in Capsicum fruit
title_sort phytoene synthase 2 can compensate for the absence of psy1 in the control of color in capsicum fruit
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475241/
https://www.ncbi.nlm.nih.gov/pubmed/32219321
http://dx.doi.org/10.1093/jxb/eraa155
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