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Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato

Trichomes are epidermal protuberances on aerial parts of plants known to play an important role in biotic and abiotic stresses. To date, our knowledge of the regulation of trichome formation in crop species is very limited. Through phenotyping of the Solanum pennellii×S. lycopersicum (cv. M82) intro...

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Autores principales: Chen, Yao, Su, Dan, Li, Jie, Ying, Shiyu, Deng, Heng, He, Xiaoqing, Zhu, Yunqi, Li, Ying, Chen, Ya, Pirrello, Julien, Bouzayen, Mondher, Liu, Yongsheng, Liu, Mingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475245/
https://www.ncbi.nlm.nih.gov/pubmed/32133496
http://dx.doi.org/10.1093/jxb/eraa114
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author Chen, Yao
Su, Dan
Li, Jie
Ying, Shiyu
Deng, Heng
He, Xiaoqing
Zhu, Yunqi
Li, Ying
Chen, Ya
Pirrello, Julien
Bouzayen, Mondher
Liu, Yongsheng
Liu, Mingchun
author_facet Chen, Yao
Su, Dan
Li, Jie
Ying, Shiyu
Deng, Heng
He, Xiaoqing
Zhu, Yunqi
Li, Ying
Chen, Ya
Pirrello, Julien
Bouzayen, Mondher
Liu, Yongsheng
Liu, Mingchun
author_sort Chen, Yao
collection PubMed
description Trichomes are epidermal protuberances on aerial parts of plants known to play an important role in biotic and abiotic stresses. To date, our knowledge of the regulation of trichome formation in crop species is very limited. Through phenotyping of the Solanum pennellii×S. lycopersicum (cv. M82) introgression population, we identified the SlbHLH95 transcription factor as a negative regulator of trichome formation in tomato. In line with this negative role, SlbHLH95 displayed a very low expression in stems where trichomes are present at high density. Overexpression of SlbHLH95 resulted in a dramatically reduced trichome density in stems and a significant down-regulation of a set of trichome-related genes. In addition to the lower trichome density, overexpressing lines also showed pleiotropic alterations affecting both vegetative and reproductive development. While most of these phenotypes were reminiscent of gibberellin (GA)-deficient phenotypes, expression studies showed that two GA biosynthesis genes, SlGA20ox2 and SlKS5, are significantly down-regulated in SlbHLH95-OE plants. Moreover, in line with a decrease in active GA content, the glabrous and dwarf phenotypes were rescued by exogenous GA treatment. In addition, yeast one-hybrid and transactivation assays revealed that SlbHLH95 represses the expression of SlGA20ox2 and SlKS5 via direct binding to their promoters. Taken together, our study established a link between SlbHLH95, GA, and trichome formation, and uncovered the role of this gene in modulating GA biosynthesis in tomato.
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spelling pubmed-74752452020-09-10 Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato Chen, Yao Su, Dan Li, Jie Ying, Shiyu Deng, Heng He, Xiaoqing Zhu, Yunqi Li, Ying Chen, Ya Pirrello, Julien Bouzayen, Mondher Liu, Yongsheng Liu, Mingchun J Exp Bot Research Papers Trichomes are epidermal protuberances on aerial parts of plants known to play an important role in biotic and abiotic stresses. To date, our knowledge of the regulation of trichome formation in crop species is very limited. Through phenotyping of the Solanum pennellii×S. lycopersicum (cv. M82) introgression population, we identified the SlbHLH95 transcription factor as a negative regulator of trichome formation in tomato. In line with this negative role, SlbHLH95 displayed a very low expression in stems where trichomes are present at high density. Overexpression of SlbHLH95 resulted in a dramatically reduced trichome density in stems and a significant down-regulation of a set of trichome-related genes. In addition to the lower trichome density, overexpressing lines also showed pleiotropic alterations affecting both vegetative and reproductive development. While most of these phenotypes were reminiscent of gibberellin (GA)-deficient phenotypes, expression studies showed that two GA biosynthesis genes, SlGA20ox2 and SlKS5, are significantly down-regulated in SlbHLH95-OE plants. Moreover, in line with a decrease in active GA content, the glabrous and dwarf phenotypes were rescued by exogenous GA treatment. In addition, yeast one-hybrid and transactivation assays revealed that SlbHLH95 represses the expression of SlGA20ox2 and SlKS5 via direct binding to their promoters. Taken together, our study established a link between SlbHLH95, GA, and trichome formation, and uncovered the role of this gene in modulating GA biosynthesis in tomato. Oxford University Press 2020-06-22 2020-03-05 /pmc/articles/PMC7475245/ /pubmed/32133496 http://dx.doi.org/10.1093/jxb/eraa114 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Chen, Yao
Su, Dan
Li, Jie
Ying, Shiyu
Deng, Heng
He, Xiaoqing
Zhu, Yunqi
Li, Ying
Chen, Ya
Pirrello, Julien
Bouzayen, Mondher
Liu, Yongsheng
Liu, Mingchun
Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title_full Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title_fullStr Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title_full_unstemmed Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title_short Overexpression of bHLH95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
title_sort overexpression of bhlh95, a basic helix–loop–helix transcription factor family member, impacts trichome formation via regulating gibberellin biosynthesis in tomato
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475245/
https://www.ncbi.nlm.nih.gov/pubmed/32133496
http://dx.doi.org/10.1093/jxb/eraa114
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