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Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions

Low gibberellin (GA) activity in tomato (Solanum lycopersicum) inhibits leaf expansion and reduces stomatal conductance. This leads to lower transpiration and improved water status under transient drought conditions. Tomato has three GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors with overlappin...

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Autores principales: Illouz-Eliaz, Natanella, Nissan, Idan, Nir, Ido, Ramon, Uria, Shohat, Hagai, Weiss, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475260/
https://www.ncbi.nlm.nih.gov/pubmed/32173726
http://dx.doi.org/10.1093/jxb/eraa137
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author Illouz-Eliaz, Natanella
Nissan, Idan
Nir, Ido
Ramon, Uria
Shohat, Hagai
Weiss, David
author_facet Illouz-Eliaz, Natanella
Nissan, Idan
Nir, Ido
Ramon, Uria
Shohat, Hagai
Weiss, David
author_sort Illouz-Eliaz, Natanella
collection PubMed
description Low gibberellin (GA) activity in tomato (Solanum lycopersicum) inhibits leaf expansion and reduces stomatal conductance. This leads to lower transpiration and improved water status under transient drought conditions. Tomato has three GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors with overlapping activities and high redundancy. We tested whether mutation in a single GID1 reduces transpiration without affecting growth and productivity. CRISPR-Cas9 gid1 mutants were able to maintain higher leaf water content under water-deficit conditions. Moreover, while gid1a exhibited normal growth, it showed reduced whole-plant transpiration and better recovery from dehydration. Mutation in GID1a inhibited xylem vessel proliferation, which led to lower hydraulic conductance. In stronger GA mutants, we also found reduced xylem vessel expansion. These results suggest that low GA activity affects transpiration by multiple mechanisms: it reduces leaf area, promotes stomatal closure, and reduces xylem proliferation and expansion, and as a result, xylem hydraulic conductance. We further examined if gid1a performs better than the control M82 in the field. Under these conditions, the high redundancy of GID1s was lost and gid1a plants were semi-dwarf, but their productivity was not affected. Although gid1a did not perform better under drought conditions in the field, it exhibited a higher harvest index.
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spelling pubmed-74752602020-09-10 Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions Illouz-Eliaz, Natanella Nissan, Idan Nir, Ido Ramon, Uria Shohat, Hagai Weiss, David J Exp Bot Research Papers Low gibberellin (GA) activity in tomato (Solanum lycopersicum) inhibits leaf expansion and reduces stomatal conductance. This leads to lower transpiration and improved water status under transient drought conditions. Tomato has three GIBBERELLIN-INSENSITIVE DWARF1 (GID1) GA receptors with overlapping activities and high redundancy. We tested whether mutation in a single GID1 reduces transpiration without affecting growth and productivity. CRISPR-Cas9 gid1 mutants were able to maintain higher leaf water content under water-deficit conditions. Moreover, while gid1a exhibited normal growth, it showed reduced whole-plant transpiration and better recovery from dehydration. Mutation in GID1a inhibited xylem vessel proliferation, which led to lower hydraulic conductance. In stronger GA mutants, we also found reduced xylem vessel expansion. These results suggest that low GA activity affects transpiration by multiple mechanisms: it reduces leaf area, promotes stomatal closure, and reduces xylem proliferation and expansion, and as a result, xylem hydraulic conductance. We further examined if gid1a performs better than the control M82 in the field. Under these conditions, the high redundancy of GID1s was lost and gid1a plants were semi-dwarf, but their productivity was not affected. Although gid1a did not perform better under drought conditions in the field, it exhibited a higher harvest index. Oxford University Press 2020-06-22 2020-03-16 /pmc/articles/PMC7475260/ /pubmed/32173726 http://dx.doi.org/10.1093/jxb/eraa137 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Illouz-Eliaz, Natanella
Nissan, Idan
Nir, Ido
Ramon, Uria
Shohat, Hagai
Weiss, David
Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title_full Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title_fullStr Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title_full_unstemmed Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title_short Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
title_sort mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475260/
https://www.ncbi.nlm.nih.gov/pubmed/32173726
http://dx.doi.org/10.1093/jxb/eraa137
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