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Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells

Metal implants used in trauma surgeries are sometimes difficult to remove after the completion of the healing process due to the strong integration with the bone tissue. Periodic surface micro- and nanostructures can directly influence cell adhesion and differentiation on metallic implant materials....

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Autores principales: Böker, Kai Oliver, Kleinwort, Frederick, Klein-Wiele, Jan-Hendrick, Simon, Peter, Jäckle, Katharina, Taheri, Shahed, Lehmann, Wolfgang, Schilling, Arndt F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475978/
https://www.ncbi.nlm.nih.gov/pubmed/32785067
http://dx.doi.org/10.3390/ma13163526
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author Böker, Kai Oliver
Kleinwort, Frederick
Klein-Wiele, Jan-Hendrick
Simon, Peter
Jäckle, Katharina
Taheri, Shahed
Lehmann, Wolfgang
Schilling, Arndt F.
author_facet Böker, Kai Oliver
Kleinwort, Frederick
Klein-Wiele, Jan-Hendrick
Simon, Peter
Jäckle, Katharina
Taheri, Shahed
Lehmann, Wolfgang
Schilling, Arndt F.
author_sort Böker, Kai Oliver
collection PubMed
description Metal implants used in trauma surgeries are sometimes difficult to remove after the completion of the healing process due to the strong integration with the bone tissue. Periodic surface micro- and nanostructures can directly influence cell adhesion and differentiation on metallic implant materials. However, the fabrication of such structures with classical lithographic methods is too slow and cost-intensive to be of practical relevance. Therefore, we used laser beam interference ablation structuring to systematically generate periodic nanostructures on titanium and steel plates. The newly developed laser process uses a special grating interferometer in combination with an industrial laser scanner and ultrashort pulse laser source, allowing for fast, precise, and cost-effective modification of metal surfaces in a single step process. A total of 30 different periodic topologies reaching from linear over crossed to complex crossed nanostructures with varying depths were generated on steel and titanium plates and tested in bone cell culture. Reduced cell adhesion was found for four different structure types, while cell morphology was influenced by two different structures. Furthermore, we observed impaired osteogenic differentiation for three structures, indicating reduced bone formation around the implant. This efficient way of surface structuring in combination with new insights about its influence on bone cells could lead to newly designed implant surfaces for trauma surgeries with reduced adhesion, resulting in faster removal times, reduced operation times, and reduced complication rates.
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spelling pubmed-74759782020-09-09 Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells Böker, Kai Oliver Kleinwort, Frederick Klein-Wiele, Jan-Hendrick Simon, Peter Jäckle, Katharina Taheri, Shahed Lehmann, Wolfgang Schilling, Arndt F. Materials (Basel) Article Metal implants used in trauma surgeries are sometimes difficult to remove after the completion of the healing process due to the strong integration with the bone tissue. Periodic surface micro- and nanostructures can directly influence cell adhesion and differentiation on metallic implant materials. However, the fabrication of such structures with classical lithographic methods is too slow and cost-intensive to be of practical relevance. Therefore, we used laser beam interference ablation structuring to systematically generate periodic nanostructures on titanium and steel plates. The newly developed laser process uses a special grating interferometer in combination with an industrial laser scanner and ultrashort pulse laser source, allowing for fast, precise, and cost-effective modification of metal surfaces in a single step process. A total of 30 different periodic topologies reaching from linear over crossed to complex crossed nanostructures with varying depths were generated on steel and titanium plates and tested in bone cell culture. Reduced cell adhesion was found for four different structure types, while cell morphology was influenced by two different structures. Furthermore, we observed impaired osteogenic differentiation for three structures, indicating reduced bone formation around the implant. This efficient way of surface structuring in combination with new insights about its influence on bone cells could lead to newly designed implant surfaces for trauma surgeries with reduced adhesion, resulting in faster removal times, reduced operation times, and reduced complication rates. MDPI 2020-08-10 /pmc/articles/PMC7475978/ /pubmed/32785067 http://dx.doi.org/10.3390/ma13163526 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Böker, Kai Oliver
Kleinwort, Frederick
Klein-Wiele, Jan-Hendrick
Simon, Peter
Jäckle, Katharina
Taheri, Shahed
Lehmann, Wolfgang
Schilling, Arndt F.
Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title_full Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title_fullStr Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title_full_unstemmed Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title_short Laser Ablated Periodic Nanostructures on Titanium and Steel Implants Influence Adhesion and Osteogenic Differentiation of Mesenchymal Stem Cells
title_sort laser ablated periodic nanostructures on titanium and steel implants influence adhesion and osteogenic differentiation of mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475978/
https://www.ncbi.nlm.nih.gov/pubmed/32785067
http://dx.doi.org/10.3390/ma13163526
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