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Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †

Background: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria suppor...

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Autores principales: Mikulás, Krisztina, Komlódi, Timea, Földes, Anna, Sváb, Gergely, Horváth, Gergő, Nagy, Ádám Miklós, Ambrus, Attila, Gyulai-Gaál, Szabolcs, Gera, István, Hermann, Péter, Varga, Gábor, Tretter, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475988/
https://www.ncbi.nlm.nih.gov/pubmed/32781723
http://dx.doi.org/10.3390/ma13163472
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author Mikulás, Krisztina
Komlódi, Timea
Földes, Anna
Sváb, Gergely
Horváth, Gergő
Nagy, Ádám Miklós
Ambrus, Attila
Gyulai-Gaál, Szabolcs
Gera, István
Hermann, Péter
Varga, Gábor
Tretter, László
author_facet Mikulás, Krisztina
Komlódi, Timea
Földes, Anna
Sváb, Gergely
Horváth, Gergő
Nagy, Ádám Miklós
Ambrus, Attila
Gyulai-Gaál, Szabolcs
Gera, István
Hermann, Péter
Varga, Gábor
Tretter, László
author_sort Mikulás, Krisztina
collection PubMed
description Background: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria supported by CI, substrates oxidation and ATP synthesis were inhibited, reactive oxygen species production was stimulated. Contrary to that, respiratory Complex II was not impaired by TEGDMA. The beneficial effects of electron carrier compound methylene blue (MB) are proven in many disease models where mitochondrial involvement has been detected. In the present study, the bioenergetic effects of MB on TEGDMA-treated isolated mitochondria and on human dental pulp stem cells (DPSC) were analyzed. Methods: Isolated mitochondria and DPSC were acutely exposed to low millimolar concentrations of TEGDMA and 2 μM concentration of MB. Mitochondrial and cellular respiration and glycolytic flux were measured by high resolution respirometry and by Seahorse XF extracellular analyzer. Mitochondrial membrane potential was measured fluorimetrically. Results: MB partially restored the mitochondrial oxidation, rescued membrane potential in isolated mitochondria and significantly increased the impaired cellular O(2) consumption in the presence of TEGDMA. Conclusion: MB is able to protect against TEGDMA-induced CI damage, and might provide protective effects in resin monomer exposed cells.
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spelling pubmed-74759882020-09-09 Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue † Mikulás, Krisztina Komlódi, Timea Földes, Anna Sváb, Gergely Horváth, Gergő Nagy, Ádám Miklós Ambrus, Attila Gyulai-Gaál, Szabolcs Gera, István Hermann, Péter Varga, Gábor Tretter, László Materials (Basel) Article Background: Triethylene glycol dimethacrylate (TEGDMA) monomers released from resin matrix are toxic to dental pulp cells, induce apoptosis, oxidative stress and decrease viability. Recently, mitochondrial complex I (CI) was identified as a potential target of TEGDMA. In isolated mitochondria supported by CI, substrates oxidation and ATP synthesis were inhibited, reactive oxygen species production was stimulated. Contrary to that, respiratory Complex II was not impaired by TEGDMA. The beneficial effects of electron carrier compound methylene blue (MB) are proven in many disease models where mitochondrial involvement has been detected. In the present study, the bioenergetic effects of MB on TEGDMA-treated isolated mitochondria and on human dental pulp stem cells (DPSC) were analyzed. Methods: Isolated mitochondria and DPSC were acutely exposed to low millimolar concentrations of TEGDMA and 2 μM concentration of MB. Mitochondrial and cellular respiration and glycolytic flux were measured by high resolution respirometry and by Seahorse XF extracellular analyzer. Mitochondrial membrane potential was measured fluorimetrically. Results: MB partially restored the mitochondrial oxidation, rescued membrane potential in isolated mitochondria and significantly increased the impaired cellular O(2) consumption in the presence of TEGDMA. Conclusion: MB is able to protect against TEGDMA-induced CI damage, and might provide protective effects in resin monomer exposed cells. MDPI 2020-08-06 /pmc/articles/PMC7475988/ /pubmed/32781723 http://dx.doi.org/10.3390/ma13163472 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mikulás, Krisztina
Komlódi, Timea
Földes, Anna
Sváb, Gergely
Horváth, Gergő
Nagy, Ádám Miklós
Ambrus, Attila
Gyulai-Gaál, Szabolcs
Gera, István
Hermann, Péter
Varga, Gábor
Tretter, László
Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title_full Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title_fullStr Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title_full_unstemmed Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title_short Bioenergetic Impairment of Triethylene Glycol Dimethacrylate- (TEGDMA-) Treated Dental Pulp Stem Cells (DPSCs) and Isolated Brain Mitochondria are Amended by Redox Compound Methylene Blue †
title_sort bioenergetic impairment of triethylene glycol dimethacrylate- (tegdma-) treated dental pulp stem cells (dpscs) and isolated brain mitochondria are amended by redox compound methylene blue †
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7475988/
https://www.ncbi.nlm.nih.gov/pubmed/32781723
http://dx.doi.org/10.3390/ma13163472
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