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Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

Newcastle disease (ND) is a viral disease that causes labored breathing, periorbital oedema, and ataxia in the majority of avian species. The available vaccines against Newcastle disease virus (NDV) are limited, owing to their low reactivity and multiple dosage requirements. Plant-based machinery pr...

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Autores principales: Shahid, Naila, Samiullah, Tahir Rehman, Shakoor, Sana, Latif, Ayesha, Yasmeen, Aneela, Azam, Saira, Shahid, Ahmad Ali, Husnain, Tayyab, Rao, Abdul Qayyum
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476220/
https://www.ncbi.nlm.nih.gov/pubmed/33062645
http://dx.doi.org/10.3389/fvets.2020.00499
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author Shahid, Naila
Samiullah, Tahir Rehman
Shakoor, Sana
Latif, Ayesha
Yasmeen, Aneela
Azam, Saira
Shahid, Ahmad Ali
Husnain, Tayyab
Rao, Abdul Qayyum
author_facet Shahid, Naila
Samiullah, Tahir Rehman
Shakoor, Sana
Latif, Ayesha
Yasmeen, Aneela
Azam, Saira
Shahid, Ahmad Ali
Husnain, Tayyab
Rao, Abdul Qayyum
author_sort Shahid, Naila
collection PubMed
description Newcastle disease (ND) is a viral disease that causes labored breathing, periorbital oedema, and ataxia in the majority of avian species. The available vaccines against Newcastle disease virus (NDV) are limited, owing to their low reactivity and multiple dosage requirements. Plant-based machinery provides an attractive and safe system for vaccine production. In the current study, we attempted to express fusion (F) and hemagglutinin-neuraminidase (HN) proteins (the protective antigens against NDV) under constitutive 35S and seed-specific Zein promoters, respectively. Almost 2–7.1-fold higher expression of F gene mRNA in transgenic corn leaves and 8–28-fold higher expression of HN gene mRNA in transgenic corn seeds were observed, when the expression was analyzed by real-time PCR on a relative basis as compared to non-transgenic control plant material (Leaves and seeds). Similarly, 1.66 μg/ml of F protein in corn leaves, i.e., 0.5% of total soluble protein, and 2.4 μg/ml of HN protein in corn seed, i.e., 0.8% of total seed protein, were found when calculated through ELISA. Similar levels of immunological response were generated in chicks immunized through injection of E. coli-produced pET F and pET HN protein as in chickens orally fed leaves and seeds of maize with expressed immunogenic protein. Moreover, the detection of anti-NDV antibodies in the sera of chickens that were fed maize with immunogenic protein, and the absence of these antibodies in chickens fed a normal diet, confirmed the specificity of the antibodies generated through feeding, and demonstrated the potential of utilizing plants for producing more vaccine doses, vaccine generation at higher levels and against other infectious diseases.
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spelling pubmed-74762202020-10-13 Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn Shahid, Naila Samiullah, Tahir Rehman Shakoor, Sana Latif, Ayesha Yasmeen, Aneela Azam, Saira Shahid, Ahmad Ali Husnain, Tayyab Rao, Abdul Qayyum Front Vet Sci Veterinary Science Newcastle disease (ND) is a viral disease that causes labored breathing, periorbital oedema, and ataxia in the majority of avian species. The available vaccines against Newcastle disease virus (NDV) are limited, owing to their low reactivity and multiple dosage requirements. Plant-based machinery provides an attractive and safe system for vaccine production. In the current study, we attempted to express fusion (F) and hemagglutinin-neuraminidase (HN) proteins (the protective antigens against NDV) under constitutive 35S and seed-specific Zein promoters, respectively. Almost 2–7.1-fold higher expression of F gene mRNA in transgenic corn leaves and 8–28-fold higher expression of HN gene mRNA in transgenic corn seeds were observed, when the expression was analyzed by real-time PCR on a relative basis as compared to non-transgenic control plant material (Leaves and seeds). Similarly, 1.66 μg/ml of F protein in corn leaves, i.e., 0.5% of total soluble protein, and 2.4 μg/ml of HN protein in corn seed, i.e., 0.8% of total seed protein, were found when calculated through ELISA. Similar levels of immunological response were generated in chicks immunized through injection of E. coli-produced pET F and pET HN protein as in chickens orally fed leaves and seeds of maize with expressed immunogenic protein. Moreover, the detection of anti-NDV antibodies in the sera of chickens that were fed maize with immunogenic protein, and the absence of these antibodies in chickens fed a normal diet, confirmed the specificity of the antibodies generated through feeding, and demonstrated the potential of utilizing plants for producing more vaccine doses, vaccine generation at higher levels and against other infectious diseases. Frontiers Media S.A. 2020-08-24 /pmc/articles/PMC7476220/ /pubmed/33062645 http://dx.doi.org/10.3389/fvets.2020.00499 Text en Copyright © 2020 Shahid, Samiullah, Shakoor, Latif, Yasmeen, Azam, Shahid, Husnain and Rao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Shahid, Naila
Samiullah, Tahir Rehman
Shakoor, Sana
Latif, Ayesha
Yasmeen, Aneela
Azam, Saira
Shahid, Ahmad Ali
Husnain, Tayyab
Rao, Abdul Qayyum
Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title_full Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title_fullStr Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title_full_unstemmed Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title_short Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn
title_sort early stage development of a newcastle disease vaccine candidate in corn
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476220/
https://www.ncbi.nlm.nih.gov/pubmed/33062645
http://dx.doi.org/10.3389/fvets.2020.00499
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