Cargando…
Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae
Mycoplasma hyopneumoniae is the respiratory pathogen of porcine enzootic pneumonia, a chronic respiratory infectious disease that causes substantial pecuniary losses to pig husbandry worldwide. Commercial bacterins only provide incomplete protection and do not prevent the colonization and transmissi...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476235/ https://www.ncbi.nlm.nih.gov/pubmed/32923730 http://dx.doi.org/10.1016/j.heliyon.2020.e04832 |
_version_ | 1783579665859870720 |
---|---|
author | Ning, Yaru Zhou, Yaoqin Wang, Zhaodi Wen, Yukang Xu, Zuobo Tian, Yaqin Yang, Mei Wang, Xudong Yang, Yujiao Ding, Honglei |
author_facet | Ning, Yaru Zhou, Yaoqin Wang, Zhaodi Wen, Yukang Xu, Zuobo Tian, Yaqin Yang, Mei Wang, Xudong Yang, Yujiao Ding, Honglei |
author_sort | Ning, Yaru |
collection | PubMed |
description | Mycoplasma hyopneumoniae is the respiratory pathogen of porcine enzootic pneumonia, a chronic respiratory infectious disease that causes substantial pecuniary losses to pig husbandry worldwide. Commercial bacterins only provide incomplete protection and do not prevent the colonization and transmission of M. hyopneumoniae. Identification of new protective antigens is a key imperative for the development of more effective novel vaccine. The objective of this study was to evaluate antibody responses of 27 recombinant proteins in convalescent sera obtained from pigs that were naturally infected with M. hyopneumoniae. Fifteen proteins were identified as serological immunodominant antigens, while 3 proteins were not recognized by any convalescent serum. Moreover, Mhp462, a leucine aminopeptidase, was found to be a discriminative serological immunodominant antigen which reacted with convalescent sera but not with hyperimmune sera. The serological immunodominant proteins were antigenic and were expressed during infection; this suggests that these proteins (especially the discriminative one) are potential candidate antigens for the development of next generation vaccines against M. hyopneumoniae. |
format | Online Article Text |
id | pubmed-7476235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-74762352020-09-11 Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae Ning, Yaru Zhou, Yaoqin Wang, Zhaodi Wen, Yukang Xu, Zuobo Tian, Yaqin Yang, Mei Wang, Xudong Yang, Yujiao Ding, Honglei Heliyon Article Mycoplasma hyopneumoniae is the respiratory pathogen of porcine enzootic pneumonia, a chronic respiratory infectious disease that causes substantial pecuniary losses to pig husbandry worldwide. Commercial bacterins only provide incomplete protection and do not prevent the colonization and transmission of M. hyopneumoniae. Identification of new protective antigens is a key imperative for the development of more effective novel vaccine. The objective of this study was to evaluate antibody responses of 27 recombinant proteins in convalescent sera obtained from pigs that were naturally infected with M. hyopneumoniae. Fifteen proteins were identified as serological immunodominant antigens, while 3 proteins were not recognized by any convalescent serum. Moreover, Mhp462, a leucine aminopeptidase, was found to be a discriminative serological immunodominant antigen which reacted with convalescent sera but not with hyperimmune sera. The serological immunodominant proteins were antigenic and were expressed during infection; this suggests that these proteins (especially the discriminative one) are potential candidate antigens for the development of next generation vaccines against M. hyopneumoniae. Elsevier 2020-08-31 /pmc/articles/PMC7476235/ /pubmed/32923730 http://dx.doi.org/10.1016/j.heliyon.2020.e04832 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Ning, Yaru Zhou, Yaoqin Wang, Zhaodi Wen, Yukang Xu, Zuobo Tian, Yaqin Yang, Mei Wang, Xudong Yang, Yujiao Ding, Honglei Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title | Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title_full | Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title_fullStr | Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title_full_unstemmed | Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title_short | Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae |
title_sort | elevated mhp462 antibody induced by natural infection but not in vitro culture of mycoplasma hyopneumoniae |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476235/ https://www.ncbi.nlm.nih.gov/pubmed/32923730 http://dx.doi.org/10.1016/j.heliyon.2020.e04832 |
work_keys_str_mv | AT ningyaru elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT zhouyaoqin elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT wangzhaodi elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT wenyukang elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT xuzuobo elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT tianyaqin elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT yangmei elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT wangxudong elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT yangyujiao elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae AT dinghonglei elevatedmhp462antibodyinducedbynaturalinfectionbutnotinvitrocultureofmycoplasmahyopneumoniae |