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Metabolic response of blood vessels to TNFα

TNFα signaling in the vascular endothelium elicits multiple inflammatory responses that drive vascular destabilization and leakage. Bioactive lipids are main drivers of these processes. In vitro mechanistic studies of bioactive lipids have been largely based on two-dimensional endothelial cell cultu...

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Autores principales: Junaid, Abidemi, Schoeman, Johannes, Yang, Wei, Stam, Wendy, Mashaghi, Alireza, van Zonneveld, Anton Jan, Hankemeier, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476757/
https://www.ncbi.nlm.nih.gov/pubmed/32749215
http://dx.doi.org/10.7554/eLife.54754
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author Junaid, Abidemi
Schoeman, Johannes
Yang, Wei
Stam, Wendy
Mashaghi, Alireza
van Zonneveld, Anton Jan
Hankemeier, Thomas
author_facet Junaid, Abidemi
Schoeman, Johannes
Yang, Wei
Stam, Wendy
Mashaghi, Alireza
van Zonneveld, Anton Jan
Hankemeier, Thomas
author_sort Junaid, Abidemi
collection PubMed
description TNFα signaling in the vascular endothelium elicits multiple inflammatory responses that drive vascular destabilization and leakage. Bioactive lipids are main drivers of these processes. In vitro mechanistic studies of bioactive lipids have been largely based on two-dimensional endothelial cell cultures that, due to lack of laminar flow and the growth of the cells on non-compliant stiff substrates, often display a pro-inflammatory phenotype. This complicates the assessment of inflammatory processes. Three-dimensional microvessels-on-a-chip models provide a unique opportunity to generate endothelial microvessels in a more physiological environment. Using an optimized targeted liquid chromatography–tandem mass spectrometry measurements of a panel of pro- and anti-inflammatory bioactive lipids, we measure the profile changes upon administration of TNFα. We demonstrate that bioactive lipid profiles can be readily detected from three-dimensional microvessels-on-a-chip and display a more dynamic, less inflammatory response to TNFα, that resembles more the human situation, compared to classical two-dimensional endothelial cell cultures.
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spelling pubmed-74767572020-09-09 Metabolic response of blood vessels to TNFα Junaid, Abidemi Schoeman, Johannes Yang, Wei Stam, Wendy Mashaghi, Alireza van Zonneveld, Anton Jan Hankemeier, Thomas eLife Medicine TNFα signaling in the vascular endothelium elicits multiple inflammatory responses that drive vascular destabilization and leakage. Bioactive lipids are main drivers of these processes. In vitro mechanistic studies of bioactive lipids have been largely based on two-dimensional endothelial cell cultures that, due to lack of laminar flow and the growth of the cells on non-compliant stiff substrates, often display a pro-inflammatory phenotype. This complicates the assessment of inflammatory processes. Three-dimensional microvessels-on-a-chip models provide a unique opportunity to generate endothelial microvessels in a more physiological environment. Using an optimized targeted liquid chromatography–tandem mass spectrometry measurements of a panel of pro- and anti-inflammatory bioactive lipids, we measure the profile changes upon administration of TNFα. We demonstrate that bioactive lipid profiles can be readily detected from three-dimensional microvessels-on-a-chip and display a more dynamic, less inflammatory response to TNFα, that resembles more the human situation, compared to classical two-dimensional endothelial cell cultures. eLife Sciences Publications, Ltd 2020-08-04 /pmc/articles/PMC7476757/ /pubmed/32749215 http://dx.doi.org/10.7554/eLife.54754 Text en © 2020, Junaid et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Medicine
Junaid, Abidemi
Schoeman, Johannes
Yang, Wei
Stam, Wendy
Mashaghi, Alireza
van Zonneveld, Anton Jan
Hankemeier, Thomas
Metabolic response of blood vessels to TNFα
title Metabolic response of blood vessels to TNFα
title_full Metabolic response of blood vessels to TNFα
title_fullStr Metabolic response of blood vessels to TNFα
title_full_unstemmed Metabolic response of blood vessels to TNFα
title_short Metabolic response of blood vessels to TNFα
title_sort metabolic response of blood vessels to tnfα
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7476757/
https://www.ncbi.nlm.nih.gov/pubmed/32749215
http://dx.doi.org/10.7554/eLife.54754
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