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Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study

BACKGROUND: To explore the potential of synchrotron radiation (SR) phase contrast imaging (PCI) for real-time microbubble formation monitoring during radiofrequency ablation (RFA). METHODS: RFA was performed on ex vivo porcine muscle tissue using unipolar and multi-tined expandable electrodes. Image...

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Autores principales: Huang, Wei, Lu, Jian, Tang, Rongbiao, Wu, Zhiyuan, Wang, Qingbing, Ding, Xiaoyi, Wang, Zhongmin, Chen, Kemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477093/
https://www.ncbi.nlm.nih.gov/pubmed/32984051
http://dx.doi.org/10.3389/fonc.2020.01709
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author Huang, Wei
Lu, Jian
Tang, Rongbiao
Wu, Zhiyuan
Wang, Qingbing
Ding, Xiaoyi
Wang, Zhongmin
Chen, Kemin
author_facet Huang, Wei
Lu, Jian
Tang, Rongbiao
Wu, Zhiyuan
Wang, Qingbing
Ding, Xiaoyi
Wang, Zhongmin
Chen, Kemin
author_sort Huang, Wei
collection PubMed
description BACKGROUND: To explore the potential of synchrotron radiation (SR) phase contrast imaging (PCI) for real-time microbubble formation monitoring during radiofrequency ablation (RFA). METHODS: RFA was performed on ex vivo porcine muscle tissue using unipolar and multi-tined expandable electrodes. Images of microbubble formation in the samples were captured by both SR PCI and absorption contrast imaging. The synchronous ablation temperature was recorded. Each RFA electrode type group contained 6 samples. Ablation size was assessed by histologic examination. RESULTS: Microbubble formation during RFA could be visualized by SR PCI. The diameter of the microbubbles revealed on the image ranged from tens of microns to several millimeters, and these microbubbles first appeared at the edge of the RFA electrode when the target region temperature reached approximately 60°C and rapidly extended outwards. The average microbubble range measured on PCI was 17.66 ± 0.74 mm. The average range of coagulation necrosis measured by histological examination was 17.22 ± 0.38 mm. There was no significant difference between them (P > 0.05). The range of microbubbles corresponded to the ablation zone. CONCLUSION: PCI enabled real-time high-resolution visualization of microbubble formation during RFA, indicating a potential for its use in ablation monitoring.
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spelling pubmed-74770932020-09-26 Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study Huang, Wei Lu, Jian Tang, Rongbiao Wu, Zhiyuan Wang, Qingbing Ding, Xiaoyi Wang, Zhongmin Chen, Kemin Front Oncol Oncology BACKGROUND: To explore the potential of synchrotron radiation (SR) phase contrast imaging (PCI) for real-time microbubble formation monitoring during radiofrequency ablation (RFA). METHODS: RFA was performed on ex vivo porcine muscle tissue using unipolar and multi-tined expandable electrodes. Images of microbubble formation in the samples were captured by both SR PCI and absorption contrast imaging. The synchronous ablation temperature was recorded. Each RFA electrode type group contained 6 samples. Ablation size was assessed by histologic examination. RESULTS: Microbubble formation during RFA could be visualized by SR PCI. The diameter of the microbubbles revealed on the image ranged from tens of microns to several millimeters, and these microbubbles first appeared at the edge of the RFA electrode when the target region temperature reached approximately 60°C and rapidly extended outwards. The average microbubble range measured on PCI was 17.66 ± 0.74 mm. The average range of coagulation necrosis measured by histological examination was 17.22 ± 0.38 mm. There was no significant difference between them (P > 0.05). The range of microbubbles corresponded to the ablation zone. CONCLUSION: PCI enabled real-time high-resolution visualization of microbubble formation during RFA, indicating a potential for its use in ablation monitoring. Frontiers Media S.A. 2020-08-25 /pmc/articles/PMC7477093/ /pubmed/32984051 http://dx.doi.org/10.3389/fonc.2020.01709 Text en Copyright © 2020 Huang, Lu, Tang, Wu, Wang, Ding, Wang and Chen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Huang, Wei
Lu, Jian
Tang, Rongbiao
Wu, Zhiyuan
Wang, Qingbing
Ding, Xiaoyi
Wang, Zhongmin
Chen, Kemin
Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title_full Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title_fullStr Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title_full_unstemmed Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title_short Phase Contrast Imaging Based Microbubble Monitoring of Radiofrequency Ablation: An ex vivo Study
title_sort phase contrast imaging based microbubble monitoring of radiofrequency ablation: an ex vivo study
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477093/
https://www.ncbi.nlm.nih.gov/pubmed/32984051
http://dx.doi.org/10.3389/fonc.2020.01709
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