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Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)

The in vitro sperm quality parameters (motility, M; viability, V; normal morphology, NM; plasma membrane integrity, PMI; mitochondrial function, MF) in Muscovy drakes (Cairina moschata) were evaluated by using microscopy and flow cytometry, the correlation among sperm quality parameters and results...

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Autores principales: Chen, Yi-Chen, Liu, Hsiu-Chou, Wei, Liang-Yuan, Huang, Jeng-Fang, Lin, Chai-Ching, Blesbois, Elisabeth, Chen, Ming-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477139/
https://www.ncbi.nlm.nih.gov/pubmed/32908388
http://dx.doi.org/10.2141/jpsa.0150162
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author Chen, Yi-Chen
Liu, Hsiu-Chou
Wei, Liang-Yuan
Huang, Jeng-Fang
Lin, Chai-Ching
Blesbois, Elisabeth
Chen, Ming-Cheng
author_facet Chen, Yi-Chen
Liu, Hsiu-Chou
Wei, Liang-Yuan
Huang, Jeng-Fang
Lin, Chai-Ching
Blesbois, Elisabeth
Chen, Ming-Cheng
author_sort Chen, Yi-Chen
collection PubMed
description The in vitro sperm quality parameters (motility, M; viability, V; normal morphology, NM; plasma membrane integrity, PMI; mitochondrial function, MF) in Muscovy drakes (Cairina moschata) were evaluated by using microscopy and flow cytometry, the correlation among sperm quality parameters and results of artificial insemination was also assessed in present study. M, V and NM were detected by phase contrast microscopy assisted with eosinnigrosin staining, and PMI and MF were detected by using flow cytometry within appropriate fluorescence staining (SYBR-14/PI and R123/PI, respectively). Fertility (F), early embryonic mortality (EEM) and the survival embryo rate (SER) were assessed after the artificial insemination of Muscovy or Kaiya duck (Anas platyrhynchos) females. Sperm PMI and MF, the parameters detected by flow cytometry were positively correlated with sperm M, V, and NM, those were detected by phase contrast microscopy (P<0.05). Sperm V and PMI were negatively correlated with the percentage of early embryo mortality of Muscovy duck fertile eggs (P<0.05). The results of the present study showed the relationships among the AI results and the sperm quality parameters detected by microscopy as well as flow cytometry. In conclusion, flow cytometry assisted with microscopy can be an effective tool to evaluate in vitro sperm quality and may contribute to predict the reproductive performances of individual Muscovy drakes, which helps to improve duck production efficiency.
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spelling pubmed-74771392020-09-08 Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata) Chen, Yi-Chen Liu, Hsiu-Chou Wei, Liang-Yuan Huang, Jeng-Fang Lin, Chai-Ching Blesbois, Elisabeth Chen, Ming-Cheng J Poult Sci Full Papers The in vitro sperm quality parameters (motility, M; viability, V; normal morphology, NM; plasma membrane integrity, PMI; mitochondrial function, MF) in Muscovy drakes (Cairina moschata) were evaluated by using microscopy and flow cytometry, the correlation among sperm quality parameters and results of artificial insemination was also assessed in present study. M, V and NM were detected by phase contrast microscopy assisted with eosinnigrosin staining, and PMI and MF were detected by using flow cytometry within appropriate fluorescence staining (SYBR-14/PI and R123/PI, respectively). Fertility (F), early embryonic mortality (EEM) and the survival embryo rate (SER) were assessed after the artificial insemination of Muscovy or Kaiya duck (Anas platyrhynchos) females. Sperm PMI and MF, the parameters detected by flow cytometry were positively correlated with sperm M, V, and NM, those were detected by phase contrast microscopy (P<0.05). Sperm V and PMI were negatively correlated with the percentage of early embryo mortality of Muscovy duck fertile eggs (P<0.05). The results of the present study showed the relationships among the AI results and the sperm quality parameters detected by microscopy as well as flow cytometry. In conclusion, flow cytometry assisted with microscopy can be an effective tool to evaluate in vitro sperm quality and may contribute to predict the reproductive performances of individual Muscovy drakes, which helps to improve duck production efficiency. Japan Poultry Science Association 2016-07-25 /pmc/articles/PMC7477139/ /pubmed/32908388 http://dx.doi.org/10.2141/jpsa.0150162 Text en 2016, Japan Poultry Science Association. The Journal of Poultry Science is an Open Access journal distributed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Full Papers
Chen, Yi-Chen
Liu, Hsiu-Chou
Wei, Liang-Yuan
Huang, Jeng-Fang
Lin, Chai-Ching
Blesbois, Elisabeth
Chen, Ming-Cheng
Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title_full Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title_fullStr Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title_full_unstemmed Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title_short Sperm Quality Parameters and Reproductive Efficiency in Muscovy Duck (Cairina moschata)
title_sort sperm quality parameters and reproductive efficiency in muscovy duck (cairina moschata)
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477139/
https://www.ncbi.nlm.nih.gov/pubmed/32908388
http://dx.doi.org/10.2141/jpsa.0150162
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