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Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga
Lipid astaxanthin, a potent antioxidant known as a natural sunscreen, accumulates in eukaryotic microalgae and confers photoprotection. We previously identified a photooxidative stress-inducible water-soluble astaxanthin-binding carotenoprotein (AstaP) in a eukaryotic microalga (Coelastrella astaxan...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477208/ https://www.ncbi.nlm.nih.gov/pubmed/32895456 http://dx.doi.org/10.1038/s42003-020-01206-7 |
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author | Kawasaki, Shinji Yamazaki, Keita Nishikata, Tohya Ishige, Taichiro Toyoshima, Hiroki Miyata, Ami |
author_facet | Kawasaki, Shinji Yamazaki, Keita Nishikata, Tohya Ishige, Taichiro Toyoshima, Hiroki Miyata, Ami |
author_sort | Kawasaki, Shinji |
collection | PubMed |
description | Lipid astaxanthin, a potent antioxidant known as a natural sunscreen, accumulates in eukaryotic microalgae and confers photoprotection. We previously identified a photooxidative stress-inducible water-soluble astaxanthin-binding carotenoprotein (AstaP) in a eukaryotic microalga (Coelastrella astaxanthina Ki-4) isolated from an extreme environment. The distribution in eukaryotic microalgae remains unknown. Here we identified three novel AstaP orthologs in a eukaryotic microalga, Scenedesmus sp. Oki-4N. The purified proteins, named AstaP-orange2, AstaP-pink1, and AstaP-pink2, were identified as secreted fasciclin proteins with potent (1)O(2) quenching activity in aqueous solution, which are characteristics shared with Ki-4 AstaP. Nonetheless, the absence of glycosylation in the AstaP-pinks, the presence of a glycosylphosphatidylinositol (GPI) anchor motif in AstaP-orange2, and highly acidic isoelectric points (pI = 3.6–4.7), differed significantly from that of AstaP-orange1 (pI = 10.5). These results provide unique examples on the use of water-soluble forms of astaxanthin in photosynthetic organisms as novel strategies for protecting single cells against severe photooxidative stresses. |
format | Online Article Text |
id | pubmed-7477208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74772082020-09-21 Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga Kawasaki, Shinji Yamazaki, Keita Nishikata, Tohya Ishige, Taichiro Toyoshima, Hiroki Miyata, Ami Commun Biol Article Lipid astaxanthin, a potent antioxidant known as a natural sunscreen, accumulates in eukaryotic microalgae and confers photoprotection. We previously identified a photooxidative stress-inducible water-soluble astaxanthin-binding carotenoprotein (AstaP) in a eukaryotic microalga (Coelastrella astaxanthina Ki-4) isolated from an extreme environment. The distribution in eukaryotic microalgae remains unknown. Here we identified three novel AstaP orthologs in a eukaryotic microalga, Scenedesmus sp. Oki-4N. The purified proteins, named AstaP-orange2, AstaP-pink1, and AstaP-pink2, were identified as secreted fasciclin proteins with potent (1)O(2) quenching activity in aqueous solution, which are characteristics shared with Ki-4 AstaP. Nonetheless, the absence of glycosylation in the AstaP-pinks, the presence of a glycosylphosphatidylinositol (GPI) anchor motif in AstaP-orange2, and highly acidic isoelectric points (pI = 3.6–4.7), differed significantly from that of AstaP-orange1 (pI = 10.5). These results provide unique examples on the use of water-soluble forms of astaxanthin in photosynthetic organisms as novel strategies for protecting single cells against severe photooxidative stresses. Nature Publishing Group UK 2020-09-07 /pmc/articles/PMC7477208/ /pubmed/32895456 http://dx.doi.org/10.1038/s42003-020-01206-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kawasaki, Shinji Yamazaki, Keita Nishikata, Tohya Ishige, Taichiro Toyoshima, Hiroki Miyata, Ami Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title | Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title_full | Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title_fullStr | Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title_full_unstemmed | Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title_short | Photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
title_sort | photooxidative stress-inducible orange and pink water-soluble astaxanthin-binding proteins in eukaryotic microalga |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477208/ https://www.ncbi.nlm.nih.gov/pubmed/32895456 http://dx.doi.org/10.1038/s42003-020-01206-7 |
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