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Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage
Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477311/ https://www.ncbi.nlm.nih.gov/pubmed/32984068 http://dx.doi.org/10.3389/fcimb.2020.00435 |
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author | Bundgaard-Nielsen, Caspar Ammitzbøll, Nadia Isse, Yusuf Abdi Muqtar, Abdisalam Jensen, Ann-Maria Leutscher, Peter D. C. Arenholt, Louise Thomsen Schmidt Hagstrøm, Søren Sørensen, Suzette |
author_facet | Bundgaard-Nielsen, Caspar Ammitzbøll, Nadia Isse, Yusuf Abdi Muqtar, Abdisalam Jensen, Ann-Maria Leutscher, Peter D. C. Arenholt, Louise Thomsen Schmidt Hagstrøm, Søren Sørensen, Suzette |
author_sort | Bundgaard-Nielsen, Caspar |
collection | PubMed |
description | Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition. Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals. Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota. |
format | Online Article Text |
id | pubmed-7477311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74773112020-09-26 Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage Bundgaard-Nielsen, Caspar Ammitzbøll, Nadia Isse, Yusuf Abdi Muqtar, Abdisalam Jensen, Ann-Maria Leutscher, Peter D. C. Arenholt, Louise Thomsen Schmidt Hagstrøm, Søren Sørensen, Suzette Front Cell Infect Microbiol Cellular and Infection Microbiology Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition. Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals. Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota. Frontiers Media S.A. 2020-08-25 /pmc/articles/PMC7477311/ /pubmed/32984068 http://dx.doi.org/10.3389/fcimb.2020.00435 Text en Copyright © 2020 Bundgaard-Nielsen, Ammitzbøll, Isse, Muqtar, Jensen, Leutscher, Arenholt, Hagstrøm and Sørensen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Bundgaard-Nielsen, Caspar Ammitzbøll, Nadia Isse, Yusuf Abdi Muqtar, Abdisalam Jensen, Ann-Maria Leutscher, Peter D. C. Arenholt, Louise Thomsen Schmidt Hagstrøm, Søren Sørensen, Suzette Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title_full | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title_fullStr | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title_full_unstemmed | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title_short | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
title_sort | voided urinary microbiota is stable over time but impacted by post void storage |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477311/ https://www.ncbi.nlm.nih.gov/pubmed/32984068 http://dx.doi.org/10.3389/fcimb.2020.00435 |
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