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Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC

Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute systemic vasculitis syndrome that mainly occurs in infants under 5 years of age. In the current manuscript, we were aiming to analyze the role of neutrophil extracellular traps (NETs) in the pathogenesis of KD, espec...

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Autores principales: Jing, Yang, Ding, Meng, Fu, Jiuyuan, Xiao, Yanping, Chen, Xianghua, Zhang, Qingyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477316/
https://www.ncbi.nlm.nih.gov/pubmed/32808647
http://dx.doi.org/10.1042/BSR20200928
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author Jing, Yang
Ding, Meng
Fu, Jiuyuan
Xiao, Yanping
Chen, Xianghua
Zhang, Qingyun
author_facet Jing, Yang
Ding, Meng
Fu, Jiuyuan
Xiao, Yanping
Chen, Xianghua
Zhang, Qingyun
author_sort Jing, Yang
collection PubMed
description Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute systemic vasculitis syndrome that mainly occurs in infants under 5 years of age. In the current manuscript, we were aiming to analyze the role of neutrophil extracellular traps (NETs) in the pathogenesis of KD, especially their interplay with peripheral blood mononuclear cells (PBMCs). Neutrophils were exposed to 20 nM phorbol myristate acetate (PMA), we found that neutrophils of KD patients were more likely to form NETs compared with healthy controls (HCs). Furthermore, PBMCs were cultured with NETs for 24 h, and we observed that NETs significantly increased the cell viability, suppressed cell apoptosis, and enhanced the pro-inflammatory cytokines production and NF-κB activation in PBMCs from KD patients. In addition, with the stimulation of NETs, the expression of vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α) were increased, which were related with the pathological mechanism of KD. At last, we examined the activation of phosphoinositide 3 kinase (PI3K)/Akt signaling, and we found NETs treatment obviously enhanced the activation of PI3K and Akt. In conclusion, these findings suggested that the formation of NETs may alter the biologic responses of PBMC and affect the vascular injury in KD.
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spelling pubmed-74773162020-09-18 Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC Jing, Yang Ding, Meng Fu, Jiuyuan Xiao, Yanping Chen, Xianghua Zhang, Qingyun Biosci Rep Cardiovascular System & Vascular Biology Kawasaki disease (KD), also known as mucocutaneous lymph node syndrome, is an acute systemic vasculitis syndrome that mainly occurs in infants under 5 years of age. In the current manuscript, we were aiming to analyze the role of neutrophil extracellular traps (NETs) in the pathogenesis of KD, especially their interplay with peripheral blood mononuclear cells (PBMCs). Neutrophils were exposed to 20 nM phorbol myristate acetate (PMA), we found that neutrophils of KD patients were more likely to form NETs compared with healthy controls (HCs). Furthermore, PBMCs were cultured with NETs for 24 h, and we observed that NETs significantly increased the cell viability, suppressed cell apoptosis, and enhanced the pro-inflammatory cytokines production and NF-κB activation in PBMCs from KD patients. In addition, with the stimulation of NETs, the expression of vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (HIF-1α) were increased, which were related with the pathological mechanism of KD. At last, we examined the activation of phosphoinositide 3 kinase (PI3K)/Akt signaling, and we found NETs treatment obviously enhanced the activation of PI3K and Akt. In conclusion, these findings suggested that the formation of NETs may alter the biologic responses of PBMC and affect the vascular injury in KD. Portland Press Ltd. 2020-09-07 /pmc/articles/PMC7477316/ /pubmed/32808647 http://dx.doi.org/10.1042/BSR20200928 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Cardiovascular System & Vascular Biology
Jing, Yang
Ding, Meng
Fu, Jiuyuan
Xiao, Yanping
Chen, Xianghua
Zhang, Qingyun
Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title_full Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title_fullStr Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title_full_unstemmed Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title_short Neutrophil extracellular trap from Kawasaki disease alter the biologic responses of PBMC
title_sort neutrophil extracellular trap from kawasaki disease alter the biologic responses of pbmc
topic Cardiovascular System & Vascular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477316/
https://www.ncbi.nlm.nih.gov/pubmed/32808647
http://dx.doi.org/10.1042/BSR20200928
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