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Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran

BACKGROUND & OBJECTIVE: Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDL-C in clinical laboratories. In order to decrease the cost, instead of its measuring, it is recommended to calculate it using m...

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Autores principales: Atabi, Fereshteh, Mohammadi, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477686/
https://www.ncbi.nlm.nih.gov/pubmed/32944037
http://dx.doi.org/10.30699/ijp.2020.110379.2174
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author Atabi, Fereshteh
Mohammadi, Reza
author_facet Atabi, Fereshteh
Mohammadi, Reza
author_sort Atabi, Fereshteh
collection PubMed
description BACKGROUND & OBJECTIVE: Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDL-C in clinical laboratories. In order to decrease the cost, instead of its measuring, it is recommended to calculate it using multiple formulas that have been introduced up to now. The aim of this study was to assess the results of various formulas and comparison of these results with those of measuring method and to clarify the best formula for the Iranian population. METHODS: Concentrations of total cholesterol (TC), triglyceride (TG), cholesterol of high-density lipoprotein (HDL-C) and LDL-C in serums of 471 overnight fasting individuals were measured and also LDL-Cs of these samples were calculated by eleven different formulas according to their TC, TG, and HDL-C concentrations. Subsequently, results of measured and calculated LDL-C were analyzed statistically by paired t-test, correlation coefficient, and Passing-Bablok regression. In addition, for clinical evaluation, the differences between calculated and measured mean results were calculated and compared with an allowable total error. RESULTS: Paired t-test unraveled a significant difference between the results of measured and calculated LDL-C by various formulas. But for some formulas, these differences were not clinically significant. The best clinical and statistical agreement (correlation coefficient) was obtained by the Friedewald equation. CONCLUSION: By using validated methods which have correct calibration and control system for measuring TC, TG, and HDL-C, we can use the Friedewald formula for calculating LDL-C in serum samples with TG up to 400 mg/dL.
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spelling pubmed-74776862020-09-16 Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran Atabi, Fereshteh Mohammadi, Reza Iran J Pathol Original Article BACKGROUND & OBJECTIVE: Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDL-C in clinical laboratories. In order to decrease the cost, instead of its measuring, it is recommended to calculate it using multiple formulas that have been introduced up to now. The aim of this study was to assess the results of various formulas and comparison of these results with those of measuring method and to clarify the best formula for the Iranian population. METHODS: Concentrations of total cholesterol (TC), triglyceride (TG), cholesterol of high-density lipoprotein (HDL-C) and LDL-C in serums of 471 overnight fasting individuals were measured and also LDL-Cs of these samples were calculated by eleven different formulas according to their TC, TG, and HDL-C concentrations. Subsequently, results of measured and calculated LDL-C were analyzed statistically by paired t-test, correlation coefficient, and Passing-Bablok regression. In addition, for clinical evaluation, the differences between calculated and measured mean results were calculated and compared with an allowable total error. RESULTS: Paired t-test unraveled a significant difference between the results of measured and calculated LDL-C by various formulas. But for some formulas, these differences were not clinically significant. The best clinical and statistical agreement (correlation coefficient) was obtained by the Friedewald equation. CONCLUSION: By using validated methods which have correct calibration and control system for measuring TC, TG, and HDL-C, we can use the Friedewald formula for calculating LDL-C in serum samples with TG up to 400 mg/dL. Iranian Society of Pathology 2020 2020-07-18 /pmc/articles/PMC7477686/ /pubmed/32944037 http://dx.doi.org/10.30699/ijp.2020.110379.2174 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Atabi, Fereshteh
Mohammadi, Reza
Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title_full Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title_fullStr Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title_full_unstemmed Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title_short Clinical Validation of Eleven Formulas for Calculating LDL-C in Iran
title_sort clinical validation of eleven formulas for calculating ldl-c in iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477686/
https://www.ncbi.nlm.nih.gov/pubmed/32944037
http://dx.doi.org/10.30699/ijp.2020.110379.2174
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