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Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR

BACKGROUND & OBJECTIVE: A simple approach to prevent close contact in healthcare settings during the COVID-19 outbreak is to train patients to collect their own nasopharyngeal and oropharyngeal swabs and deliver them to medical laboratories to have them processed. The aim of our study was to com...

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Autores principales: Abdollahi, Alireza, shakoori, Abbas, Khoshnevis, Hoda, Arabzadeh, Mohammad, Dehghan Manshadi, Seyed Ali, Mohammadnejad, Esmaeil, Ghasemi, Dorsa, Safari Aboksari, Maryam, Alizadeh, Shaban, Mehrtash, Vahid, Eftekhar-javadi, Arezoo, Safaei, Masoomeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Society of Pathology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477688/
https://www.ncbi.nlm.nih.gov/pubmed/32944044
http://dx.doi.org/10.30699/ijp.2020.127312.2387
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author Abdollahi, Alireza
shakoori, Abbas
Khoshnevis, Hoda
Arabzadeh, Mohammad
Dehghan Manshadi, Seyed Ali
Mohammadnejad, Esmaeil
Ghasemi, Dorsa
Safari Aboksari, Maryam
Alizadeh, Shaban
Mehrtash, Vahid
Eftekhar-javadi, Arezoo
Safaei, Masoomeh
author_facet Abdollahi, Alireza
shakoori, Abbas
Khoshnevis, Hoda
Arabzadeh, Mohammad
Dehghan Manshadi, Seyed Ali
Mohammadnejad, Esmaeil
Ghasemi, Dorsa
Safari Aboksari, Maryam
Alizadeh, Shaban
Mehrtash, Vahid
Eftekhar-javadi, Arezoo
Safaei, Masoomeh
author_sort Abdollahi, Alireza
collection PubMed
description BACKGROUND & OBJECTIVE: A simple approach to prevent close contact in healthcare settings during the COVID-19 outbreak is to train patients to collect their own nasopharyngeal and oropharyngeal swabs and deliver them to medical laboratories to have them processed. The aim of our study was to compare lab technician- with patient- collected oropharyngeal and nasopharyngeal samples for detection of the coronavirus disease 2019 (COVID 19) using rapid real-time polymerase chain reaction (rRT-PCR). METHODS: Fifty adult patients with flu-like symptoms and radiologic findings compatible with atypical pneumonia who were admitted to the infectious diseases ward of Imam Khomeini Hospital Complex, Tehran, Iran, with a clinical diagnosis of COVID-19 from February 28 to April 27 of 2020 were randomly selected and entered in our study. Two sets of naso- and oropharyngeal swabs were collected, one set by a lab technician and the other by the patients, and the COVID-19 rRT-PCR test was performed. RESULTS: Of 50 selected cases, in seven patients all collected naso- and oropharyngeal swabs tested positive, and in 22 patients all samples tested negative for COVID-19 in rRT-PCR. Discrepancies between rRT-PCR results of lab technician- and patient-collected swabs were observed in 12 nasopharyngeal and 13 oropharyngeal specimens. Positive lab technician-collected and negative patient-collected samples were observed in 10 and 5 nasopharyngeal and oropharyngeal specimens, respectively. Negative lab technician-collected and positive patient-collected samples were observed in two and seven nasopharyngeal and oropharyngeal specimens, respectively. The overall percentage of agreement among both nasopharyngeal and oropharyngeal swabs taken by a lab technician and patients was 76% with a kappa value of 0.49 (P=0.001). CONCLUSION: Based on our findings, lab technician-collected naso- and oropharyngeal swabs cannot be replaced by patient-collected ones with regard to COVID-19 rRT-PCR.
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spelling pubmed-74776882020-09-16 Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR Abdollahi, Alireza shakoori, Abbas Khoshnevis, Hoda Arabzadeh, Mohammad Dehghan Manshadi, Seyed Ali Mohammadnejad, Esmaeil Ghasemi, Dorsa Safari Aboksari, Maryam Alizadeh, Shaban Mehrtash, Vahid Eftekhar-javadi, Arezoo Safaei, Masoomeh Iran J Pathol Original Article BACKGROUND & OBJECTIVE: A simple approach to prevent close contact in healthcare settings during the COVID-19 outbreak is to train patients to collect their own nasopharyngeal and oropharyngeal swabs and deliver them to medical laboratories to have them processed. The aim of our study was to compare lab technician- with patient- collected oropharyngeal and nasopharyngeal samples for detection of the coronavirus disease 2019 (COVID 19) using rapid real-time polymerase chain reaction (rRT-PCR). METHODS: Fifty adult patients with flu-like symptoms and radiologic findings compatible with atypical pneumonia who were admitted to the infectious diseases ward of Imam Khomeini Hospital Complex, Tehran, Iran, with a clinical diagnosis of COVID-19 from February 28 to April 27 of 2020 were randomly selected and entered in our study. Two sets of naso- and oropharyngeal swabs were collected, one set by a lab technician and the other by the patients, and the COVID-19 rRT-PCR test was performed. RESULTS: Of 50 selected cases, in seven patients all collected naso- and oropharyngeal swabs tested positive, and in 22 patients all samples tested negative for COVID-19 in rRT-PCR. Discrepancies between rRT-PCR results of lab technician- and patient-collected swabs were observed in 12 nasopharyngeal and 13 oropharyngeal specimens. Positive lab technician-collected and negative patient-collected samples were observed in 10 and 5 nasopharyngeal and oropharyngeal specimens, respectively. Negative lab technician-collected and positive patient-collected samples were observed in two and seven nasopharyngeal and oropharyngeal specimens, respectively. The overall percentage of agreement among both nasopharyngeal and oropharyngeal swabs taken by a lab technician and patients was 76% with a kappa value of 0.49 (P=0.001). CONCLUSION: Based on our findings, lab technician-collected naso- and oropharyngeal swabs cannot be replaced by patient-collected ones with regard to COVID-19 rRT-PCR. Iranian Society of Pathology 2020 2020-07-16 /pmc/articles/PMC7477688/ /pubmed/32944044 http://dx.doi.org/10.30699/ijp.2020.127312.2387 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Abdollahi, Alireza
shakoori, Abbas
Khoshnevis, Hoda
Arabzadeh, Mohammad
Dehghan Manshadi, Seyed Ali
Mohammadnejad, Esmaeil
Ghasemi, Dorsa
Safari Aboksari, Maryam
Alizadeh, Shaban
Mehrtash, Vahid
Eftekhar-javadi, Arezoo
Safaei, Masoomeh
Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title_full Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title_fullStr Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title_full_unstemmed Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title_short Comparison of Patient-collected and Lab Technician-collected Nasopharyngeal and Oropharyngeal Swabs for Detection of COVID-19 by RT-PCR
title_sort comparison of patient-collected and lab technician-collected nasopharyngeal and oropharyngeal swabs for detection of covid-19 by rt-pcr
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477688/
https://www.ncbi.nlm.nih.gov/pubmed/32944044
http://dx.doi.org/10.30699/ijp.2020.127312.2387
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