Cargando…

Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China

Salmonella Weltevreden is increasingly reported from aquatic environments, seafood, and patients in several Southeast Asian countries. Using genome-wide analysis, we characterized S. Weltevreden isolated from cultured shrimp and tilapia from Vietnam and China to study their genetic characteristics a...

Descripción completa

Detalles Bibliográficos
Autores principales: Hounmanou, Yaovi M. G., Dalsgaard, Anders, Sopacua, Tirzania Frannetta, Uddin, Gazi Md. Noor, Leekitcharoenphon, Pimlapas, Hendriksen, Rene S., Olsen, John E., Larsen, Marianne Halberg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477899/
https://www.ncbi.nlm.nih.gov/pubmed/32983012
http://dx.doi.org/10.3389/fmicb.2020.01985
_version_ 1783579973862293504
author Hounmanou, Yaovi M. G.
Dalsgaard, Anders
Sopacua, Tirzania Frannetta
Uddin, Gazi Md. Noor
Leekitcharoenphon, Pimlapas
Hendriksen, Rene S.
Olsen, John E.
Larsen, Marianne Halberg
author_facet Hounmanou, Yaovi M. G.
Dalsgaard, Anders
Sopacua, Tirzania Frannetta
Uddin, Gazi Md. Noor
Leekitcharoenphon, Pimlapas
Hendriksen, Rene S.
Olsen, John E.
Larsen, Marianne Halberg
author_sort Hounmanou, Yaovi M. G.
collection PubMed
description Salmonella Weltevreden is increasingly reported from aquatic environments, seafood, and patients in several Southeast Asian countries. Using genome-wide analysis, we characterized S. Weltevreden isolated from cultured shrimp and tilapia from Vietnam and China to study their genetic characteristics and relatedness to clinical isolates of S. Weltevreden ST-365. The phylogenetic analysis revealed up to 312 single-nucleotide polymorphism (SNP) difference between tilapia isolates, whereas isolates from shrimp were genetically more closely related. Epidemiologically unrelated isolates from Vietnam were closely related to isolates from China, e.g., 20 SNPs differences between strains 28V and 75C. In comparison with strains from other parts of the world, our environmental isolates predominantly clustered within the continental South Asia lineage, constituted mostly of strains from human stool with as low as seven SNPs difference, e.g., 30V versus Cont_ERR495254. All sequenced isolates were MLST type ST-365 and contained the major virulence-related genes encoded by the Salmonella Pathogenicity Islands 1–5. Ten of the isolates harbored the IncFII(S) plasmid similar to the virulence genes-mediated plasmid pSPCV of S. Paratyphi C, and one isolate had the IncQ1 plasmid on the same contig with strA/B, sul2, and tetA resistance genes similar to the IncQ1 type, pNUC of S. Typhimurium. A pangenomic analysis yielded 7891 genes including a core genome of 4892 genes, with a closely related accessory genome content between clinical and environmental isolates (Benjamini p > 0.05). In a search for differences that could explain the higher prevalence of S. Weltevreden in aquatic samples, genomes were compared with those of other Salmonella enterica serovars. S. Weltevreden revealed specific regions harboring glpX (Fructose-1;6-bisphosphatase; class II), rfbC (dTDP-4-dehydrorhamnose 3;5-epimerase), and cmtB (PTS Mannitol-specific cryptic phosphotransferase enzyme IIA component) involved in carbohydrate biosynthesis pathways. Our study builds grounds for future experiments to determine genes or pathways that are essential when S. Weltevreden are in aquatic environments and microbial interactions providing survival advantages to S. Weltevreden in such environments.
format Online
Article
Text
id pubmed-7477899
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-74778992020-09-26 Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China Hounmanou, Yaovi M. G. Dalsgaard, Anders Sopacua, Tirzania Frannetta Uddin, Gazi Md. Noor Leekitcharoenphon, Pimlapas Hendriksen, Rene S. Olsen, John E. Larsen, Marianne Halberg Front Microbiol Microbiology Salmonella Weltevreden is increasingly reported from aquatic environments, seafood, and patients in several Southeast Asian countries. Using genome-wide analysis, we characterized S. Weltevreden isolated from cultured shrimp and tilapia from Vietnam and China to study their genetic characteristics and relatedness to clinical isolates of S. Weltevreden ST-365. The phylogenetic analysis revealed up to 312 single-nucleotide polymorphism (SNP) difference between tilapia isolates, whereas isolates from shrimp were genetically more closely related. Epidemiologically unrelated isolates from Vietnam were closely related to isolates from China, e.g., 20 SNPs differences between strains 28V and 75C. In comparison with strains from other parts of the world, our environmental isolates predominantly clustered within the continental South Asia lineage, constituted mostly of strains from human stool with as low as seven SNPs difference, e.g., 30V versus Cont_ERR495254. All sequenced isolates were MLST type ST-365 and contained the major virulence-related genes encoded by the Salmonella Pathogenicity Islands 1–5. Ten of the isolates harbored the IncFII(S) plasmid similar to the virulence genes-mediated plasmid pSPCV of S. Paratyphi C, and one isolate had the IncQ1 plasmid on the same contig with strA/B, sul2, and tetA resistance genes similar to the IncQ1 type, pNUC of S. Typhimurium. A pangenomic analysis yielded 7891 genes including a core genome of 4892 genes, with a closely related accessory genome content between clinical and environmental isolates (Benjamini p > 0.05). In a search for differences that could explain the higher prevalence of S. Weltevreden in aquatic samples, genomes were compared with those of other Salmonella enterica serovars. S. Weltevreden revealed specific regions harboring glpX (Fructose-1;6-bisphosphatase; class II), rfbC (dTDP-4-dehydrorhamnose 3;5-epimerase), and cmtB (PTS Mannitol-specific cryptic phosphotransferase enzyme IIA component) involved in carbohydrate biosynthesis pathways. Our study builds grounds for future experiments to determine genes or pathways that are essential when S. Weltevreden are in aquatic environments and microbial interactions providing survival advantages to S. Weltevreden in such environments. Frontiers Media S.A. 2020-08-25 /pmc/articles/PMC7477899/ /pubmed/32983012 http://dx.doi.org/10.3389/fmicb.2020.01985 Text en Copyright © 2020 Hounmanou, Dalsgaard, Sopacua, Uddin, Leekitcharoenphon, Hendriksen, Olsen and Larsen. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hounmanou, Yaovi M. G.
Dalsgaard, Anders
Sopacua, Tirzania Frannetta
Uddin, Gazi Md. Noor
Leekitcharoenphon, Pimlapas
Hendriksen, Rene S.
Olsen, John E.
Larsen, Marianne Halberg
Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title_full Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title_fullStr Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title_full_unstemmed Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title_short Molecular Characteristics and Zoonotic Potential of Salmonella Weltevreden From Cultured Shrimp and Tilapia in Vietnam and China
title_sort molecular characteristics and zoonotic potential of salmonella weltevreden from cultured shrimp and tilapia in vietnam and china
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7477899/
https://www.ncbi.nlm.nih.gov/pubmed/32983012
http://dx.doi.org/10.3389/fmicb.2020.01985
work_keys_str_mv AT hounmanouyaovimg molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT dalsgaardanders molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT sopacuatirzaniafrannetta molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT uddingazimdnoor molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT leekitcharoenphonpimlapas molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT hendriksenrenes molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT olsenjohne molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina
AT larsenmariannehalberg molecularcharacteristicsandzoonoticpotentialofsalmonellaweltevredenfromculturedshrimpandtilapiainvietnamandchina