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Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells

OBJECTIVE(S): Adipose tissue is one of the most important endocrine organs that liberates many metabolic mediators such as hormones, cytokines, and chemokines. Different types of fatty acids have key roles in adipogenesis. The aim of this study was to evaluate the effects of two essential fatty acid...

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Autores principales: Mostoli, Rezvan, Goudarzi, Farjam, Mohammadalipour, Adel, Khodadadi, Iraj, Goodarzi, Mohammad Taghi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478257/
https://www.ncbi.nlm.nih.gov/pubmed/32952949
http://dx.doi.org/10.22038/ijbms.2020.41557.9819
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author Mostoli, Rezvan
Goudarzi, Farjam
Mohammadalipour, Adel
Khodadadi, Iraj
Goodarzi, Mohammad Taghi
author_facet Mostoli, Rezvan
Goudarzi, Farjam
Mohammadalipour, Adel
Khodadadi, Iraj
Goodarzi, Mohammad Taghi
author_sort Mostoli, Rezvan
collection PubMed
description OBJECTIVE(S): Adipose tissue is one of the most important endocrine organs that liberates many metabolic mediators such as hormones, cytokines, and chemokines. Different types of fatty acids have key roles in adipogenesis. The aim of this study was to evaluate the effects of two essential fatty acids, including Arachidonic acid (AA) and Eicosapentaenoic acid (EPA), on the process of adipogenicity in human Adipose-Derived Stem Cells (hADSCs). MATERIALS AND METHODS: After immunophenotyping of hADSCs by flowcytometry, they were differentiated into adipocytes and simultaneously exposed to 30 μM and 60 μM of AA and 25 μM and 50 μM of EPA. Further, along with the MTS assay, the activity of glycalaldehyde-3-phosphate dehydrogenase (GAPDH) was also measured. In addition, expression of lipid markers including peroxisome proliferator-activated receptor γ2 (PPARγ2) and glucose transporter 4 (GLUT4) was evaluated, and the neutral lipid contents were determined using Oil red O staining. RESULTS: MTS evaluation showed a significant decrease in proliferation in all treatment groups compared to the control group. Based on oil red O staining, fat droplets in the AA treatment groups were higher than in controls. The expression of PPARγ2 and GLUT4 genes and proteins increased in almost all AA and EPA groups compared to control. In addition, GAPDH activity was higher in AA groups than in the control group. In general, while different concentrations of EPA did not increase the adipogenic process compared to the control group, stimulation of differentiation to adipocytes was largely determined by the AA. CONCLUSION: The result indicates a positive effect of omega-6 versus omega-3 in stimulating the pathways of adipogenesis.
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spelling pubmed-74782572020-09-17 Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells Mostoli, Rezvan Goudarzi, Farjam Mohammadalipour, Adel Khodadadi, Iraj Goodarzi, Mohammad Taghi Iran J Basic Med Sci Original Article OBJECTIVE(S): Adipose tissue is one of the most important endocrine organs that liberates many metabolic mediators such as hormones, cytokines, and chemokines. Different types of fatty acids have key roles in adipogenesis. The aim of this study was to evaluate the effects of two essential fatty acids, including Arachidonic acid (AA) and Eicosapentaenoic acid (EPA), on the process of adipogenicity in human Adipose-Derived Stem Cells (hADSCs). MATERIALS AND METHODS: After immunophenotyping of hADSCs by flowcytometry, they were differentiated into adipocytes and simultaneously exposed to 30 μM and 60 μM of AA and 25 μM and 50 μM of EPA. Further, along with the MTS assay, the activity of glycalaldehyde-3-phosphate dehydrogenase (GAPDH) was also measured. In addition, expression of lipid markers including peroxisome proliferator-activated receptor γ2 (PPARγ2) and glucose transporter 4 (GLUT4) was evaluated, and the neutral lipid contents were determined using Oil red O staining. RESULTS: MTS evaluation showed a significant decrease in proliferation in all treatment groups compared to the control group. Based on oil red O staining, fat droplets in the AA treatment groups were higher than in controls. The expression of PPARγ2 and GLUT4 genes and proteins increased in almost all AA and EPA groups compared to control. In addition, GAPDH activity was higher in AA groups than in the control group. In general, while different concentrations of EPA did not increase the adipogenic process compared to the control group, stimulation of differentiation to adipocytes was largely determined by the AA. CONCLUSION: The result indicates a positive effect of omega-6 versus omega-3 in stimulating the pathways of adipogenesis. Mashhad University of Medical Sciences 2020-08 /pmc/articles/PMC7478257/ /pubmed/32952949 http://dx.doi.org/10.22038/ijbms.2020.41557.9819 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mostoli, Rezvan
Goudarzi, Farjam
Mohammadalipour, Adel
Khodadadi, Iraj
Goodarzi, Mohammad Taghi
Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title_full Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title_fullStr Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title_full_unstemmed Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title_short Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
title_sort evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7478257/
https://www.ncbi.nlm.nih.gov/pubmed/32952949
http://dx.doi.org/10.22038/ijbms.2020.41557.9819
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