Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells

Human NAD(P)H: quinone oxidoreductase (hNQO1) is an important biomarker for human malignant tumors. Detection of NQO1 accurately is of great significance to improve the early diagnosis of cancer and prognosis of cancer patients. In this study, based on the covalent assembly strategy, hNQO1-activated...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Jialing, Cheng, Longhao, Zhu, Ya, Xu, Xiaowei, Ge, Chaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479225/
https://www.ncbi.nlm.nih.gov/pubmed/33005608
http://dx.doi.org/10.3389/fchem.2020.00756
_version_ 1783580225437696000
author Han, Jialing
Cheng, Longhao
Zhu, Ya
Xu, Xiaowei
Ge, Chaoliang
author_facet Han, Jialing
Cheng, Longhao
Zhu, Ya
Xu, Xiaowei
Ge, Chaoliang
author_sort Han, Jialing
collection PubMed
description Human NAD(P)H: quinone oxidoreductase (hNQO1) is an important biomarker for human malignant tumors. Detection of NQO1 accurately is of great significance to improve the early diagnosis of cancer and prognosis of cancer patients. In this study, based on the covalent assembly strategy, hNQO1-activated fluorescent probes 1 and 2 are constructed by introducing coumarin precursor 2-cyano-3-(4-(diethylamino)-2-hydroxyphenyl) acrylic acid and self-immolative linkers. Under reaction with hNQO1 and NADH, turn-on fluorescence appears due to in-situ formation of the organic fluorescent compound 7-diethylamino-3-cyanocoumarin, and fluorescent intensity changes significantly. Probe 1 and 2 for detection of hNQO1 are not interfered by other substances and have low toxicity in cells. In addition to quantitative detection of hNQO1 in vitro, they have also been successfully applied to fluorescent imaging in living cells.
format Online
Article
Text
id pubmed-7479225
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-74792252020-09-30 Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells Han, Jialing Cheng, Longhao Zhu, Ya Xu, Xiaowei Ge, Chaoliang Front Chem Chemistry Human NAD(P)H: quinone oxidoreductase (hNQO1) is an important biomarker for human malignant tumors. Detection of NQO1 accurately is of great significance to improve the early diagnosis of cancer and prognosis of cancer patients. In this study, based on the covalent assembly strategy, hNQO1-activated fluorescent probes 1 and 2 are constructed by introducing coumarin precursor 2-cyano-3-(4-(diethylamino)-2-hydroxyphenyl) acrylic acid and self-immolative linkers. Under reaction with hNQO1 and NADH, turn-on fluorescence appears due to in-situ formation of the organic fluorescent compound 7-diethylamino-3-cyanocoumarin, and fluorescent intensity changes significantly. Probe 1 and 2 for detection of hNQO1 are not interfered by other substances and have low toxicity in cells. In addition to quantitative detection of hNQO1 in vitro, they have also been successfully applied to fluorescent imaging in living cells. Frontiers Media S.A. 2020-08-26 /pmc/articles/PMC7479225/ /pubmed/33005608 http://dx.doi.org/10.3389/fchem.2020.00756 Text en Copyright © 2020 Han, Cheng, Zhu, Xu and Ge. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Han, Jialing
Cheng, Longhao
Zhu, Ya
Xu, Xiaowei
Ge, Chaoliang
Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title_full Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title_fullStr Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title_full_unstemmed Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title_short Covalent-Assembly Based Fluorescent Probes for Detection of hNQO1 and Imaging in Living Cells
title_sort covalent-assembly based fluorescent probes for detection of hnqo1 and imaging in living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7479225/
https://www.ncbi.nlm.nih.gov/pubmed/33005608
http://dx.doi.org/10.3389/fchem.2020.00756
work_keys_str_mv AT hanjialing covalentassemblybasedfluorescentprobesfordetectionofhnqo1andimaginginlivingcells
AT chenglonghao covalentassemblybasedfluorescentprobesfordetectionofhnqo1andimaginginlivingcells
AT zhuya covalentassemblybasedfluorescentprobesfordetectionofhnqo1andimaginginlivingcells
AT xuxiaowei covalentassemblybasedfluorescentprobesfordetectionofhnqo1andimaginginlivingcells
AT gechaoliang covalentassemblybasedfluorescentprobesfordetectionofhnqo1andimaginginlivingcells

Ejemplares similares