Cargando…
Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator
In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA–mediated posttranscriptional and also in transcriptional gene silencing. Here, we report a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480116/ https://www.ncbi.nlm.nih.gov/pubmed/32673398 http://dx.doi.org/10.1083/jcb.201908097 |
_version_ | 1783580369521475584 |
---|---|
author | Gómez Acuña, Luciana I. Nazer, Ezequiel Rodríguez-Seguí, Santiago A. Pozzi, Berta Buggiano, Valeria Marasco, Luciano E. Agirre, Eneritz He, Cody Alló, Mariano Kornblihtt, Alberto R. |
author_facet | Gómez Acuña, Luciana I. Nazer, Ezequiel Rodríguez-Seguí, Santiago A. Pozzi, Berta Buggiano, Valeria Marasco, Luciano E. Agirre, Eneritz He, Cody Alló, Mariano Kornblihtt, Alberto R. |
author_sort | Gómez Acuña, Luciana I. |
collection | PubMed |
description | In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA–mediated posttranscriptional and also in transcriptional gene silencing. Here, we report a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells, AGO1 associates with transcriptional enhancers of estrogen receptor α (ERα) and that this association is up-regulated by treating the cells with estrogen (E2), displaying a positive correlation with the activation of these enhancers. Moreover, we show that AGO1 interacts with ERα and that this interaction is also increased by E2 treatment, but occurs in the absence of RNA. We show that AGO1 acts positively as a coactivator in estradiol-triggered transcription regulation by promoting ERα binding to its enhancers. Consistently, AGO1 depletion decreases long-range contacts between ERα enhancers and their target promoters. Our results point to a role of AGO1 in transcriptional regulation in human cells that is independent from small RNA binding. |
format | Online Article Text |
id | pubmed-7480116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-74801162021-03-07 Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator Gómez Acuña, Luciana I. Nazer, Ezequiel Rodríguez-Seguí, Santiago A. Pozzi, Berta Buggiano, Valeria Marasco, Luciano E. Agirre, Eneritz He, Cody Alló, Mariano Kornblihtt, Alberto R. J Cell Biol Report In mammals, argonaute (AGO) proteins have been characterized for their roles in small RNA–mediated posttranscriptional and also in transcriptional gene silencing. Here, we report a different role for AGO1 in estradiol-triggered transcriptional activation in human cells. We show that in MCF-7 mammary gland cells, AGO1 associates with transcriptional enhancers of estrogen receptor α (ERα) and that this association is up-regulated by treating the cells with estrogen (E2), displaying a positive correlation with the activation of these enhancers. Moreover, we show that AGO1 interacts with ERα and that this interaction is also increased by E2 treatment, but occurs in the absence of RNA. We show that AGO1 acts positively as a coactivator in estradiol-triggered transcription regulation by promoting ERα binding to its enhancers. Consistently, AGO1 depletion decreases long-range contacts between ERα enhancers and their target promoters. Our results point to a role of AGO1 in transcriptional regulation in human cells that is independent from small RNA binding. Rockefeller University Press 2020-07-16 /pmc/articles/PMC7480116/ /pubmed/32673398 http://dx.doi.org/10.1083/jcb.201908097 Text en © 2020 Gomez Acuna et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Report Gómez Acuña, Luciana I. Nazer, Ezequiel Rodríguez-Seguí, Santiago A. Pozzi, Berta Buggiano, Valeria Marasco, Luciano E. Agirre, Eneritz He, Cody Alló, Mariano Kornblihtt, Alberto R. Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title | Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title_full | Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title_fullStr | Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title_full_unstemmed | Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title_short | Nuclear role for human Argonaute-1 as an estrogen-dependent transcription coactivator |
title_sort | nuclear role for human argonaute-1 as an estrogen-dependent transcription coactivator |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480116/ https://www.ncbi.nlm.nih.gov/pubmed/32673398 http://dx.doi.org/10.1083/jcb.201908097 |
work_keys_str_mv | AT gomezacunalucianai nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT nazerezequiel nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT rodriguezseguisantiagoa nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT pozziberta nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT buggianovaleria nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT marascolucianoe nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT agirreeneritz nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT hecody nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT allomariano nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator AT kornblihttalbertor nuclearroleforhumanargonaute1asanestrogendependenttranscriptioncoactivator |