Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins

We present a simple ratio method to infer protein composition within cellular structures using proximity labeling approaches but compensating for the diffusion of free radicals. We used tyramide signal amplification (TSA) and label-free mass spectrometry (MS) to compare proteins in nuclear speckles...

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Detalles Bibliográficos
Autores principales: Dopie, Joseph, Sweredoski, Michael J., Moradian, Annie, Belmont, Andrew S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480118/
https://www.ncbi.nlm.nih.gov/pubmed/32609799
http://dx.doi.org/10.1083/jcb.201910207

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