Cargando…

Chikungunya virus infections in Finnish travellers 2009-2019

The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened...

Descripción completa

Detalles Bibliográficos
Autores principales: Jääskeläinen, A. J., Kareinen, L., Smura, T., Kallio-Kokko, H., Vapalahti, O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480415/
https://www.ncbi.nlm.nih.gov/pubmed/32944165
http://dx.doi.org/10.1080/20008686.2020.1798096
_version_ 1783580414129995776
author Jääskeläinen, A. J.
Kareinen, L.
Smura, T.
Kallio-Kokko, H.
Vapalahti, O.
author_facet Jääskeläinen, A. J.
Kareinen, L.
Smura, T.
Kallio-Kokko, H.
Vapalahti, O.
author_sort Jääskeläinen, A. J.
collection PubMed
description The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10(-8).During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15.  Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen.
format Online
Article
Text
id pubmed-7480415
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-74804152020-09-16 Chikungunya virus infections in Finnish travellers 2009-2019 Jääskeläinen, A. J. Kareinen, L. Smura, T. Kallio-Kokko, H. Vapalahti, O. Infect Ecol Epidemiol Research Article The mosquito-borne chikungunya virus (CHIKV) causes an acute febrile illness with rash, joint and muscle pain.A realtime RT-PCR assay for CHIKV detecting non-structural protein (nsP2; CHIKV nsP2-RT-qPCR) was set up. All the serodiagnosed CHIKV cases detected during 2009-2019 in Finland were screened with the assay, followed by isolations attempts and sequencing using Sanger and next generation sequencing (NGS). To validate the assay external and in-house quality control samples were used and all were correctly identified. Specificity of the assay was 100%. Assay was sensitive to detect CHIKV RNA in dilution of 10(-8).During years 2009-2019 34 patients were diagnosed for acute CHIKV infection. Twelve out of 34 cases were positive by CHIKV nsP2-RT-qPCR.Two CHIKV isolations succeeded from two individuals infected originally in Thailand, 2019. From 12 CHIKV nsP2-RT-qPCR positive samples, five (42%) CHIKVs were successfully sequenced. In this study, CHIKVs from year 2019 clustered with CHIKV ECSA-lineage forming sub-cluster with strains from ones detected in Bangladesh 2017, and the ones from Jamaica (2014) within Asian lineage showing highest similarity to strains detected in Caribbean outbreak 2013-15.  Majority of the CHIKV infections detected in Finland originates from Asia and virus lineages reflect the global circulation of the pathogen. Taylor & Francis 2020-08-26 /pmc/articles/PMC7480415/ /pubmed/32944165 http://dx.doi.org/10.1080/20008686.2020.1798096 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jääskeläinen, A. J.
Kareinen, L.
Smura, T.
Kallio-Kokko, H.
Vapalahti, O.
Chikungunya virus infections in Finnish travellers 2009-2019
title Chikungunya virus infections in Finnish travellers 2009-2019
title_full Chikungunya virus infections in Finnish travellers 2009-2019
title_fullStr Chikungunya virus infections in Finnish travellers 2009-2019
title_full_unstemmed Chikungunya virus infections in Finnish travellers 2009-2019
title_short Chikungunya virus infections in Finnish travellers 2009-2019
title_sort chikungunya virus infections in finnish travellers 2009-2019
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480415/
https://www.ncbi.nlm.nih.gov/pubmed/32944165
http://dx.doi.org/10.1080/20008686.2020.1798096
work_keys_str_mv AT jaaskelainenaj chikungunyavirusinfectionsinfinnishtravellers20092019
AT kareinenl chikungunyavirusinfectionsinfinnishtravellers20092019
AT smurat chikungunyavirusinfectionsinfinnishtravellers20092019
AT kalliokokkoh chikungunyavirusinfectionsinfinnishtravellers20092019
AT vapalahtio chikungunyavirusinfectionsinfinnishtravellers20092019