Cargando…

A novel three step protocol to isolate extracellular vesicles from plasma or cell culture medium with both high yield and purity

Extracellular vesicles (EV) are membrane encapsulated nanoparticles that can function in intercellular communication, and their presence in biofluids can be indicative for (patho)physiological conditions. Studies aiming to resolve functionalities of EV or to discover EV-associated biomarkers for dis...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zhang, Xiaogang, Borg, Ellen G. F., Liaci, A. Manuel, Vos, Harmjan R., Stoorvogel, Willem
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Taylor & Francis 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480457/ https://www.ncbi.nlm.nih.gov/pubmed/32944179 http://dx.doi.org/10.1080/20013078.2020.1791450

Ejemplares similares

Proteomic Profiling of Two Distinct Populations of Extracellular Vesicles Isolated from Human Seminal Plasma
por: Zhang, Xiaogang, et al.
Publicado: (2020)

Extracellular vesicles: Exosomes, microvesicles, and friends
por: Raposo, Graça, et al.
Publicado: (2013)

The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles
por: Cvjetkovic, Aleksander, et al.
Publicado: (2014)

Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential
por: Takov, Kaloyan, et al.
Publicado: (2018)

Optimized Protocol for the Isolation of Extracellular Vesicles from the Parasitic Worm Schistosoma mansoni with Improved Purity, Concentration, and Yield
por: Kuipers, Marije E., et al.
Publicado: (2022)

Reversible zwitterionic coordination enables rapid, high-yield, and high-purity isolation of extracellular vesicles from biofluids
por: Li, Qiang, et al.
Publicado: (2023)

Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
por: Simon, Thomas, et al.
Publicado: (2020)

Analysis of the Escherichia coli extracellular vesicle proteome identifies markers of purity and culture conditions
por: Hong, Jiwon, et al.
Publicado: (2019)

Bystander T-Cells Support Clonal T-Cell Activation by Controlling the Release of Dendritic Cell-Derived Immune-Stimulatory Extracellular Vesicles
por: Lindenbergh, Marthe F. S., et al.
Publicado: (2019)

Rapid production method with increased yield of high‐purity extracellular vesicles obtained using extended mitochondrial targeting domain peptide
por: Lim, Kyung Min, et al.
Publicado: (2022)

Purity Determines the Effect of Extracellular Vesicles Derived from Mesenchymal Stromal Cells
por: Forteza-Genestra, Maria Antònia, et al.
Publicado: (2020)

An Isolation System to Collect High Quality and Purity Extracellular Vesicles from Serum
por: Yang, Jian, et al.
Publicado: (2021)

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma
por: Onódi, Zsófia, et al.
Publicado: (2018)

Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System
por: Kim, Jongmin, et al.
Publicado: (2015)

Raman spectroscopy as a quick tool to assess purity of extracellular vesicle preparations and predict their functionality
por: Gualerzi, Alice, et al.
Publicado: (2019)

Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium
por: Dong, Liang, et al.
Publicado: (2021)

Plasma-derived extracellular vesicles yield predictive markers of cranial irradiation exposure in mice
por: Hinzman, Charles P., et al.
Publicado: (2019)

Purity and yield of melanoma exosomes are dependent on isolation method
por: Shu, Shin La, et al.
Publicado: (2019)

Extracellular Vesicle Isolation Yields Increased by Low-Temperature Gaseous Plasma Treatment of Polypropylene Tubes
por: Resnik, Matic, et al.
Publicado: (2020)

Staphylococcus aureus delta toxin modulates both extracellular membrane vesicle biogenesis and amyloid formation
por: Wang, Xiaogang, et al.
Publicado: (2023)

Quantitative Proteomics of Medium-Sized Extracellular Vesicle-Enriched Plasma of Lacunar Infarction for the Discovery of Prognostic Biomarkers
por: Datta, Arnab, et al.
Publicado: (2022)

Determinants of DNA yield and purity collected with buccal cell samples
por: van Wieren-de Wijer, D. B. M. A., et al.
Publicado: (2009)

Extracellular vesicles yield predictive pre-eclampsia biomarkers
por: Tan, Kok Hian, et al.
Publicado: (2017)

High performance anion exchange chromatography purification of probiotic bacterial extracellular vesicles enhances purity and anti-inflammatory efficacy
por: Piroli, Nicholas H., et al.
Publicado: (2023)

Defined MSC exosome with high yield and purity to improve regenerative activity
por: Kim, Jun Yong, et al.
Publicado: (2021)

Medulloblastoma Exosome Proteomics Yield Functional Roles for Extracellular Vesicles
por: Epple, Laura M., et al.
Publicado: (2012)

The Separation and Characterization of Extracellular Vesicles from Medium Conditioned by Bovine Embryos
por: Pavani, Krishna Chaitanya, et al.
Publicado: (2020)

Medium Extracellular Vesicles—A Qualitative and Quantitative Biomarker of Prostate Cancer
por: Świtońska, Milena, et al.
Publicado: (2022)

Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications
por: Patel, Girijesh Kumar, et al.
Publicado: (2019)

Mouse spleen lymphoblasts generated in vitro. Recovery in high yield and purity after floatation in dense bovine plasma albumin solutions
Publicado: (1978)

Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples
por: Karimi, Nasibeh, et al.
Publicado: (2022)

Isolation and Characterization of Functionally Active Extracellular Vesicles from Culture Medium Conditioned by Bovine Embryos In Vitro
por: Pavani, Krishna Chaitanya, et al.
Publicado: (2018)

Single-step isolation of extracellular vesicles by size-exclusion chromatography
por: Böing, Anita N., et al.
Publicado: (2014)

Increased Yield of Extracellular Vesicles after Cytochalasin B Treatment and Vortexing
por: Kurbangaleeva, Sirina V., et al.
Publicado: (2023)

Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins
por: Ter-Ovanesyan, Dmitry, et al.
Publicado: (2023)

In-Cell Labeling Coupled to Direct Analysis of Extracellular Vesicles in the Conditioned Medium to Study Extracellular Vesicles Secretion with Minimum Sample Processing and Particle Loss
por: Viveiros, Anissa, et al.
Publicado: (2022)

Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery
por: Gheinani, Ali Hashemi, et al.
Publicado: (2018)

One-Pot Production of RNA in High Yield and Purity Through Cleaving Tandem Transcripts
por: Feyrer, Hannes, et al.
Publicado: (2020)

Liposomes or Extracellular Vesicles: A Comprehensive Comparison of Both Lipid Bilayer Vesicles for Pulmonary Drug Delivery
por: Al-Jipouri, Ali, et al.
Publicado: (2023)

A Four-Step Purification Process for Gag VLPs: From Culture Supernatant to High-Purity Lyophilized Particles
por: González-Domínguez, Irene, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_lb176h4c5847768m9u8cbm5o1a