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Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs
In the fight against the outbreak of COVID-19 in China, we treated some asymptomatic infected individuals. This study aimed to detect pathogens in biological and environmental samples of these asymptomatic infected individuals and analyse their association. Using a cross-sectional study design, we c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480639/ https://www.ncbi.nlm.nih.gov/pubmed/33207437 http://dx.doi.org/10.1016/j.scitotenv.2020.142289 |
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author | Huang, Ying Ding, Zhen Chen, Qi Wu, Ling Guo, Lili Zhao, Chunhua Sha, Li Sun, Hong |
author_facet | Huang, Ying Ding, Zhen Chen, Qi Wu, Ling Guo, Lili Zhao, Chunhua Sha, Li Sun, Hong |
author_sort | Huang, Ying |
collection | PubMed |
description | In the fight against the outbreak of COVID-19 in China, we treated some asymptomatic infected individuals. This study aimed to detect pathogens in biological and environmental samples of these asymptomatic infected individuals and analyse their association. Using a cross-sectional study design, we collected biological and environmental samples from 19 patients treated in the isolation ward of Nanjing No.2 Hospital. Biological samples included saliva, pharyngeal swabs, blood, anal swabs, and exhaled breath condensate. Swab samples from the ward environment included inside masks, outside masks, palm swabs, bedside handrails, bedside tables, cell phone screens, toilet cell phone shelves, toilet pads and toilet lids. We also obtained some samples from public areas. We used RT-PCR to detect pathogens and colloidal gold to detect antibodies. As results, 19 asymptomatic infected individuals participated in the survey, with 8 positives for pathogens and 11 positives only for antibodies. Three positive samples were detected from among 96 environmental samples, respectively, from a cell phone surface, a cell phone shelf and a bedside handrail. No positive samples were detected in the exhaled breath condensate in this work. All patients identified pathogens in the environment had positive anal swabs. There was a statistical association between positive anal swabs and positive environmental samples. The association of positive samples from the surrounding of asymptomatically infected patients with positive anal swabs suggested that patients might secrete the virus for a more extended period. |
format | Online Article Text |
id | pubmed-7480639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74806392020-09-09 Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs Huang, Ying Ding, Zhen Chen, Qi Wu, Ling Guo, Lili Zhao, Chunhua Sha, Li Sun, Hong Sci Total Environ Short Communication In the fight against the outbreak of COVID-19 in China, we treated some asymptomatic infected individuals. This study aimed to detect pathogens in biological and environmental samples of these asymptomatic infected individuals and analyse their association. Using a cross-sectional study design, we collected biological and environmental samples from 19 patients treated in the isolation ward of Nanjing No.2 Hospital. Biological samples included saliva, pharyngeal swabs, blood, anal swabs, and exhaled breath condensate. Swab samples from the ward environment included inside masks, outside masks, palm swabs, bedside handrails, bedside tables, cell phone screens, toilet cell phone shelves, toilet pads and toilet lids. We also obtained some samples from public areas. We used RT-PCR to detect pathogens and colloidal gold to detect antibodies. As results, 19 asymptomatic infected individuals participated in the survey, with 8 positives for pathogens and 11 positives only for antibodies. Three positive samples were detected from among 96 environmental samples, respectively, from a cell phone surface, a cell phone shelf and a bedside handrail. No positive samples were detected in the exhaled breath condensate in this work. All patients identified pathogens in the environment had positive anal swabs. There was a statistical association between positive anal swabs and positive environmental samples. The association of positive samples from the surrounding of asymptomatically infected patients with positive anal swabs suggested that patients might secrete the virus for a more extended period. Elsevier B.V. 2021-01-20 2020-09-09 /pmc/articles/PMC7480639/ /pubmed/33207437 http://dx.doi.org/10.1016/j.scitotenv.2020.142289 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Short Communication Huang, Ying Ding, Zhen Chen, Qi Wu, Ling Guo, Lili Zhao, Chunhua Sha, Li Sun, Hong Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title | Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title_full | Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title_fullStr | Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title_full_unstemmed | Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title_short | Environmental virus detection associated with asymptomatic SARS-CoV-2-infected individuals with positive anal swabs |
title_sort | environmental virus detection associated with asymptomatic sars-cov-2-infected individuals with positive anal swabs |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480639/ https://www.ncbi.nlm.nih.gov/pubmed/33207437 http://dx.doi.org/10.1016/j.scitotenv.2020.142289 |
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