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Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods

INTRODUCTION: Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debil...

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Autores principales: Saki, Jasem, Eskandari, Elham, Feghhi, Mostafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481152/
https://www.ncbi.nlm.nih.gov/pubmed/32424529
http://dx.doi.org/10.1007/s10792-020-01393-6
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author Saki, Jasem
Eskandari, Elham
Feghhi, Mostafa
author_facet Saki, Jasem
Eskandari, Elham
Feghhi, Mostafa
author_sort Saki, Jasem
collection PubMed
description INTRODUCTION: Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debilitating vision loss.In Khuzestan province, southwest of Iran, T. gondii infection has been reported to be significant but toxocariasis was rare. However, the frequency of ocular toxoplasmosis and toxocariasis has not been studied in this area. The aim of this study was to evaluate the ocular toxoplasmosis and ocular toxocariasis using serological and molecular methods. METHOD: In this case control study, 310 patients were identified by ophthalmologist as ocular toxoplasmosis and then 5 cc of venous blood samples were taken from each of them. Serum samples and buffy coat were prepared and ELISA was used to detect IgG and IgM anti-Toxoplasma antibodies and the molecular PCR was used to detect Toxoplasma DNA parasite in buffy coats. ELISA test was used to detect of IgG anti-Toxocara antibodies. RESULTS: Totally, for ocular toxoplasmosis, 130 (41.93%) of 310 patients were positive by ELISA, of them 121 (39%) IgG positive and nine (2.9%) IgM positive were diagnosed. Of 121 cases with IgG(+), 119 (98.35%) were diagnosed with high IgG avidity indicating chronic phase of the infection. For ocular toxocariasis evaluation, antibodies against Toxocara were not detected in any of the samples. By PCR molecular method, 11 out of 310 patients (3.54%) had T. gondii DNA in the blood. In control, in total, 21 cases were detected positive by serology method, which showed a significant difference with the results of the case group(P < 0.05).By PCR method, only three cases showed positive which also indicated significant difference with result of case group (3 vs 9) (P < 0.05). In the control group, also no anti-toxocara antibodies were found. CONCLUSION: It can be concluded that T. gondii in Khuzestan province as the etiologic agent of ocular toxoplasmosis and physicians should consider diagnostic methods for identifying the infection when they visit the patients.
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spelling pubmed-74811522020-09-21 Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods Saki, Jasem Eskandari, Elham Feghhi, Mostafa Int Ophthalmol Original Paper INTRODUCTION: Toxoplasma gondii is an intracellular protozoan parasite that can cause ocular toxoplasmosis with most complications such as retinal detachment. Toxocara parasite, round worm, found in dogs and cats appears as larva migrans in humans can cause serious ocular complications such as debilitating vision loss.In Khuzestan province, southwest of Iran, T. gondii infection has been reported to be significant but toxocariasis was rare. However, the frequency of ocular toxoplasmosis and toxocariasis has not been studied in this area. The aim of this study was to evaluate the ocular toxoplasmosis and ocular toxocariasis using serological and molecular methods. METHOD: In this case control study, 310 patients were identified by ophthalmologist as ocular toxoplasmosis and then 5 cc of venous blood samples were taken from each of them. Serum samples and buffy coat were prepared and ELISA was used to detect IgG and IgM anti-Toxoplasma antibodies and the molecular PCR was used to detect Toxoplasma DNA parasite in buffy coats. ELISA test was used to detect of IgG anti-Toxocara antibodies. RESULTS: Totally, for ocular toxoplasmosis, 130 (41.93%) of 310 patients were positive by ELISA, of them 121 (39%) IgG positive and nine (2.9%) IgM positive were diagnosed. Of 121 cases with IgG(+), 119 (98.35%) were diagnosed with high IgG avidity indicating chronic phase of the infection. For ocular toxocariasis evaluation, antibodies against Toxocara were not detected in any of the samples. By PCR molecular method, 11 out of 310 patients (3.54%) had T. gondii DNA in the blood. In control, in total, 21 cases were detected positive by serology method, which showed a significant difference with the results of the case group(P < 0.05).By PCR method, only three cases showed positive which also indicated significant difference with result of case group (3 vs 9) (P < 0.05). In the control group, also no anti-toxocara antibodies were found. CONCLUSION: It can be concluded that T. gondii in Khuzestan province as the etiologic agent of ocular toxoplasmosis and physicians should consider diagnostic methods for identifying the infection when they visit the patients. Springer Netherlands 2020-05-18 2020 /pmc/articles/PMC7481152/ /pubmed/32424529 http://dx.doi.org/10.1007/s10792-020-01393-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Paper
Saki, Jasem
Eskandari, Elham
Feghhi, Mostafa
Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title_full Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title_fullStr Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title_full_unstemmed Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title_short Study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
title_sort study of toxoplasmosis and toxocariasis in patients suffering from ophthalmic disorders using serological and molecular methods
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481152/
https://www.ncbi.nlm.nih.gov/pubmed/32424529
http://dx.doi.org/10.1007/s10792-020-01393-6
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