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Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet

The discovery of ancestral RAG transposons in early deuterostomia reveals the origin of vertebrate V(D)J recombination. Here, we analyze the functional regulation of a RAG transposon, ProtoRAG, in lancelet. We find that a specific interaction between the cis-acting element within the TIR sequences o...

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Autores principales: Liu, Song, Yuan, Shaochun, Gao, Xiaoman, Tao, Xin, Yu, Wenjuan, Li, Xu, Chen, Shangwu, Xu, Anlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481187/
https://www.ncbi.nlm.nih.gov/pubmed/32908127
http://dx.doi.org/10.1038/s41467-020-18261-7
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author Liu, Song
Yuan, Shaochun
Gao, Xiaoman
Tao, Xin
Yu, Wenjuan
Li, Xu
Chen, Shangwu
Xu, Anlong
author_facet Liu, Song
Yuan, Shaochun
Gao, Xiaoman
Tao, Xin
Yu, Wenjuan
Li, Xu
Chen, Shangwu
Xu, Anlong
author_sort Liu, Song
collection PubMed
description The discovery of ancestral RAG transposons in early deuterostomia reveals the origin of vertebrate V(D)J recombination. Here, we analyze the functional regulation of a RAG transposon, ProtoRAG, in lancelet. We find that a specific interaction between the cis-acting element within the TIR sequences of ProtoRAG and a trans-acting factor, lancelet YY1-like (bbYY1), is important for the transcriptional regulation of lancelet RAG-like genes (bbRAG1L and bbRAG2L). Mechanistically, bbYY1 suppresses the transposition of ProtoRAG; meanwhile, bbYY1 promotes host DNA rejoins (HDJ) and TIR-TIR joints (TTJ) after TIR-dependent excision by facilitating the binding of bbRAG1L/2 L to TIR-containing DNA, and by interacting with the bbRAG1L/2 L complex. Our data thus suggest that bbYY1 has dual functions in fine-tuning the activity of ProtoRAG and maintaining the genome stability of the host.
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spelling pubmed-74811872020-09-21 Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet Liu, Song Yuan, Shaochun Gao, Xiaoman Tao, Xin Yu, Wenjuan Li, Xu Chen, Shangwu Xu, Anlong Nat Commun Article The discovery of ancestral RAG transposons in early deuterostomia reveals the origin of vertebrate V(D)J recombination. Here, we analyze the functional regulation of a RAG transposon, ProtoRAG, in lancelet. We find that a specific interaction between the cis-acting element within the TIR sequences of ProtoRAG and a trans-acting factor, lancelet YY1-like (bbYY1), is important for the transcriptional regulation of lancelet RAG-like genes (bbRAG1L and bbRAG2L). Mechanistically, bbYY1 suppresses the transposition of ProtoRAG; meanwhile, bbYY1 promotes host DNA rejoins (HDJ) and TIR-TIR joints (TTJ) after TIR-dependent excision by facilitating the binding of bbRAG1L/2 L to TIR-containing DNA, and by interacting with the bbRAG1L/2 L complex. Our data thus suggest that bbYY1 has dual functions in fine-tuning the activity of ProtoRAG and maintaining the genome stability of the host. Nature Publishing Group UK 2020-09-09 /pmc/articles/PMC7481187/ /pubmed/32908127 http://dx.doi.org/10.1038/s41467-020-18261-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Song
Yuan, Shaochun
Gao, Xiaoman
Tao, Xin
Yu, Wenjuan
Li, Xu
Chen, Shangwu
Xu, Anlong
Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title_full Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title_fullStr Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title_full_unstemmed Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title_short Functional regulation of an ancestral RAG transposon ProtoRAG by a trans-acting factor YY1 in lancelet
title_sort functional regulation of an ancestral rag transposon protorag by a trans-acting factor yy1 in lancelet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481187/
https://www.ncbi.nlm.nih.gov/pubmed/32908127
http://dx.doi.org/10.1038/s41467-020-18261-7
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