Cargando…

Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

Plant growth promoting rhizobacteria (PGPR) are able to provide cross-protection against multiple stress factors and facilitate growth of their plant symbionts in many ways. The aim of this study was to isolate and characterize rhizobacterial strains under natural conditions, associated with natural...

Descripción completa

Detalles Bibliográficos
Autores principales: Leontidou, Kleopatra, Genitsaris, Savvas, Papadopoulou, Anastasia, Kamou, Nathalie, Bosmali, Irene, Matsi, Theodora, Madesis, Panagiotis, Vokou, Despoina, Karamanoli, Katerina, Mellidou, Ifigeneia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481233/
https://www.ncbi.nlm.nih.gov/pubmed/32908201
http://dx.doi.org/10.1038/s41598-020-71652-0
_version_ 1783580555734941696
author Leontidou, Kleopatra
Genitsaris, Savvas
Papadopoulou, Anastasia
Kamou, Nathalie
Bosmali, Irene
Matsi, Theodora
Madesis, Panagiotis
Vokou, Despoina
Karamanoli, Katerina
Mellidou, Ifigeneia
author_facet Leontidou, Kleopatra
Genitsaris, Savvas
Papadopoulou, Anastasia
Kamou, Nathalie
Bosmali, Irene
Matsi, Theodora
Madesis, Panagiotis
Vokou, Despoina
Karamanoli, Katerina
Mellidou, Ifigeneia
author_sort Leontidou, Kleopatra
collection PubMed
description Plant growth promoting rhizobacteria (PGPR) are able to provide cross-protection against multiple stress factors and facilitate growth of their plant symbionts in many ways. The aim of this study was to isolate and characterize rhizobacterial strains under natural conditions, associated with naturally occurring representatives of wild plant species and a local tomato cultivar, growing in differently stressed Mediterranean ecosystems. A total of 85 morphologically different rhizospheric strains were isolated; twenty-five exhibited multiple in vitro PGP-associated traits, including phosphate solubilization, indole-3-acetic acid production, and 1-aminocyclopropane-1-carboxylate deaminase activity. Whole genome analysis was applied to eight selected strains for their PGP potential and assigned seven strains to Gammaproteobacteria, and one to Bacteroidetes. The genomes harboured numerous genes involved in plant growth promotion and stress regulation. They also support the notion that the presence of gene clusters with potential PGP functions is affirmative but not necessary for a strain to promote plant growth under abiotic stress conditions. The selected strains were further tested for their ability to stimulate growth under stress. This initial screening led to the identification of some strains as potential PGPR for increasing crop production in a sustainable manner.
format Online
Article
Text
id pubmed-7481233
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-74812332020-09-11 Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits Leontidou, Kleopatra Genitsaris, Savvas Papadopoulou, Anastasia Kamou, Nathalie Bosmali, Irene Matsi, Theodora Madesis, Panagiotis Vokou, Despoina Karamanoli, Katerina Mellidou, Ifigeneia Sci Rep Article Plant growth promoting rhizobacteria (PGPR) are able to provide cross-protection against multiple stress factors and facilitate growth of their plant symbionts in many ways. The aim of this study was to isolate and characterize rhizobacterial strains under natural conditions, associated with naturally occurring representatives of wild plant species and a local tomato cultivar, growing in differently stressed Mediterranean ecosystems. A total of 85 morphologically different rhizospheric strains were isolated; twenty-five exhibited multiple in vitro PGP-associated traits, including phosphate solubilization, indole-3-acetic acid production, and 1-aminocyclopropane-1-carboxylate deaminase activity. Whole genome analysis was applied to eight selected strains for their PGP potential and assigned seven strains to Gammaproteobacteria, and one to Bacteroidetes. The genomes harboured numerous genes involved in plant growth promotion and stress regulation. They also support the notion that the presence of gene clusters with potential PGP functions is affirmative but not necessary for a strain to promote plant growth under abiotic stress conditions. The selected strains were further tested for their ability to stimulate growth under stress. This initial screening led to the identification of some strains as potential PGPR for increasing crop production in a sustainable manner. Nature Publishing Group UK 2020-09-09 /pmc/articles/PMC7481233/ /pubmed/32908201 http://dx.doi.org/10.1038/s41598-020-71652-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Leontidou, Kleopatra
Genitsaris, Savvas
Papadopoulou, Anastasia
Kamou, Nathalie
Bosmali, Irene
Matsi, Theodora
Madesis, Panagiotis
Vokou, Despoina
Karamanoli, Katerina
Mellidou, Ifigeneia
Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title_full Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title_fullStr Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title_full_unstemmed Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title_short Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
title_sort plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481233/
https://www.ncbi.nlm.nih.gov/pubmed/32908201
http://dx.doi.org/10.1038/s41598-020-71652-0
work_keys_str_mv AT leontidoukleopatra plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT genitsarissavvas plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT papadopoulouanastasia plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT kamounathalie plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT bosmaliirene plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT matsitheodora plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT madesispanagiotis plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT vokoudespoina plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT karamanolikaterina plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits
AT mellidouifigeneia plantgrowthpromotingrhizobacteriaisolatedfromhalophytesanddroughttolerantplantsgenomiccharacterisationandexplorationofphytobeneficialtraits