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Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages

The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correl...

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Autores principales: Gómez-Molero, Emilia, Willis, Jesse R., Dudakova, Anna, Carreté, Laia, Weig, Michael, Groß, Uwe, Gácser, Attila, Gabaldón, Toni, Bader, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481391/
https://www.ncbi.nlm.nih.gov/pubmed/32983018
http://dx.doi.org/10.3389/fmicb.2020.01994
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author Gómez-Molero, Emilia
Willis, Jesse R.
Dudakova, Anna
Carreté, Laia
Weig, Michael
Groß, Uwe
Gácser, Attila
Gabaldón, Toni
Bader, Oliver
author_facet Gómez-Molero, Emilia
Willis, Jesse R.
Dudakova, Anna
Carreté, Laia
Weig, Michael
Groß, Uwe
Gácser, Attila
Gabaldón, Toni
Bader, Oliver
author_sort Gómez-Molero, Emilia
collection PubMed
description The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population of C. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient’s nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly in HWP1 of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination.
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spelling pubmed-74813912020-09-24 Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages Gómez-Molero, Emilia Willis, Jesse R. Dudakova, Anna Carreté, Laia Weig, Michael Groß, Uwe Gácser, Attila Gabaldón, Toni Bader, Oliver Front Microbiol Microbiology The human pathogenic yeast Candida parapsilosis has gained significant importance over the past decades as one of the principal causes of fungal bloodstream infections. Isolates of C. parapsilosis are known to be able to switch between several different colony morphologies in vitro, which are correlated with different cell shapes, altered cell surface properties, and thus different capacities to form biofilms on indwelling medical devices. In a set of six clinical specimens from a single surgery patient yielding stable smooth- as well as crepe-morphology isolates, we investigated the differences between five of them on a phenotypic and genomic level. In contrast to the initial assumption that they were switched forms of a clonal strain, karyotyping and genome sequencing showed that the patient was colonized by at least three distinct linages. Statistical analysis placed these groups distantly across the population of C. parapsilosis. Interestingly the single blood culture isolate was of smooth morphology and matched with an isolate from the patient’s nose of similar morphology. Strong variation between the isolates was seen in adhesin-encoding genes, where repeat regions showed significant variation in length and repeat-numbers, most strikingly in HWP1 of the smooth isolates. Although no differences in drug susceptibility were evident, the high phylogenetic distance separating the individual strains highlights the need for testing of multiple colonies in routine practice. The absence of biofilm formation in the blood stream isolate indicates a lack of respective adhesins in the cell wall, in turn pointing toward lack of adhesion as a positively contributing factor for dissemination. Frontiers Media S.A. 2020-08-27 /pmc/articles/PMC7481391/ /pubmed/32983018 http://dx.doi.org/10.3389/fmicb.2020.01994 Text en Copyright © 2020 Gómez-Molero, Willis, Dudakova, Carreté, Weig, Groß, Gácser, Gabaldón and Bader. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gómez-Molero, Emilia
Willis, Jesse R.
Dudakova, Anna
Carreté, Laia
Weig, Michael
Groß, Uwe
Gácser, Attila
Gabaldón, Toni
Bader, Oliver
Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title_full Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title_fullStr Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title_full_unstemmed Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title_short Phenotypic Variability in a Coinfection With Three Independent Candida parapsilosis Lineages
title_sort phenotypic variability in a coinfection with three independent candida parapsilosis lineages
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481391/
https://www.ncbi.nlm.nih.gov/pubmed/32983018
http://dx.doi.org/10.3389/fmicb.2020.01994
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