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Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)

Entomopathogenic nematodes (EPNs) can control pests due to mutualistic association with bacteria that reproduce and kill the host from septicemia, making the environment favourable for nematode development and reproduction. The objective of this study was to identify an EPN isolate collected in euca...

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Autores principales: Damascena, Alixelhe Pacheco, de Carvalho, Vanessa Rafaela, Ribeiro, Murilo Fonseca, Horta, André Ballerini, Monteiro de Castro e Castro, Bárbara, Zanuncio, Antônio José Vinha, Wilcken, Carlos Frederico, Zanuncio, José Cola, Wilcken, Silvia Renata Siciliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481718/
https://www.ncbi.nlm.nih.gov/pubmed/32968508
http://dx.doi.org/10.1098/rsos.200282
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author Damascena, Alixelhe Pacheco
de Carvalho, Vanessa Rafaela
Ribeiro, Murilo Fonseca
Horta, André Ballerini
Monteiro de Castro e Castro, Bárbara
Zanuncio, Antônio José Vinha
Wilcken, Carlos Frederico
Zanuncio, José Cola
Wilcken, Silvia Renata Siciliano
author_facet Damascena, Alixelhe Pacheco
de Carvalho, Vanessa Rafaela
Ribeiro, Murilo Fonseca
Horta, André Ballerini
Monteiro de Castro e Castro, Bárbara
Zanuncio, Antônio José Vinha
Wilcken, Carlos Frederico
Zanuncio, José Cola
Wilcken, Silvia Renata Siciliano
author_sort Damascena, Alixelhe Pacheco
collection PubMed
description Entomopathogenic nematodes (EPNs) can control pests due to mutualistic association with bacteria that reproduce and kill the host from septicemia, making the environment favourable for nematode development and reproduction. The objective of this study was to identify an EPN isolate collected in eucalyptus cultivation and to determine its pathogenicity with regard to Gonipterus platensis Marelli (Coleoptera: Curculionidae). Four steel-mesh traps with two seventh-instar Galleria mellonella larvae were buried 5 cm deep in the soil in a commercial Eucalyptus plantation. After 7 days, the traps were packed in plastic bags and transported to laboratory to isolate the EPNs using White traps. The obtained nematodes were multiplied in G. mellonella larvae and identified by sequencing their D2/D3 expansion of the 28S rDNA region by polymerase chain reaction (PCR) and specific primers for ITS regions. Steinernema diaprepesi was identified and inoculated into G. platensis pupae at doses of 500, 1000 and 5000 infective juveniles (IJs) to determine its pathogenicity to this pest. At 8 days after inoculation, the mortality rate of the G. platensis pupae was 80% with the lowest concentration and 100% with the others. The emergence of nematodes and the rapid degradation of G. platensis pupae were observed in those inoculated with IJs. The pathogenicity to the G. platensis pupae indicates potential for using this nematode in the integrated management of this insect.
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spelling pubmed-74817182020-09-22 Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae) Damascena, Alixelhe Pacheco de Carvalho, Vanessa Rafaela Ribeiro, Murilo Fonseca Horta, André Ballerini Monteiro de Castro e Castro, Bárbara Zanuncio, Antônio José Vinha Wilcken, Carlos Frederico Zanuncio, José Cola Wilcken, Silvia Renata Siciliano R Soc Open Sci Organismal and Evolutionary Biology Entomopathogenic nematodes (EPNs) can control pests due to mutualistic association with bacteria that reproduce and kill the host from septicemia, making the environment favourable for nematode development and reproduction. The objective of this study was to identify an EPN isolate collected in eucalyptus cultivation and to determine its pathogenicity with regard to Gonipterus platensis Marelli (Coleoptera: Curculionidae). Four steel-mesh traps with two seventh-instar Galleria mellonella larvae were buried 5 cm deep in the soil in a commercial Eucalyptus plantation. After 7 days, the traps were packed in plastic bags and transported to laboratory to isolate the EPNs using White traps. The obtained nematodes were multiplied in G. mellonella larvae and identified by sequencing their D2/D3 expansion of the 28S rDNA region by polymerase chain reaction (PCR) and specific primers for ITS regions. Steinernema diaprepesi was identified and inoculated into G. platensis pupae at doses of 500, 1000 and 5000 infective juveniles (IJs) to determine its pathogenicity to this pest. At 8 days after inoculation, the mortality rate of the G. platensis pupae was 80% with the lowest concentration and 100% with the others. The emergence of nematodes and the rapid degradation of G. platensis pupae were observed in those inoculated with IJs. The pathogenicity to the G. platensis pupae indicates potential for using this nematode in the integrated management of this insect. The Royal Society 2020-08-05 /pmc/articles/PMC7481718/ /pubmed/32968508 http://dx.doi.org/10.1098/rsos.200282 Text en © 2020 The Authors. http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Organismal and Evolutionary Biology
Damascena, Alixelhe Pacheco
de Carvalho, Vanessa Rafaela
Ribeiro, Murilo Fonseca
Horta, André Ballerini
Monteiro de Castro e Castro, Bárbara
Zanuncio, Antônio José Vinha
Wilcken, Carlos Frederico
Zanuncio, José Cola
Wilcken, Silvia Renata Siciliano
Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title_full Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title_fullStr Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title_full_unstemmed Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title_short Steinernema diaprepesi (Rhabditida: Steinernematidae) parasitizing Gonipterus platensis (Coleoptera: Curculionidae)
title_sort steinernema diaprepesi (rhabditida: steinernematidae) parasitizing gonipterus platensis (coleoptera: curculionidae)
topic Organismal and Evolutionary Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481718/
https://www.ncbi.nlm.nih.gov/pubmed/32968508
http://dx.doi.org/10.1098/rsos.200282
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